Ukr.Biochem.J. 2013; Volume 85, Issue 5, Sep-Oct, pp. 124-136


Oxidative stress in blood leukocytes, pro/antioxidanт status and fatty acids composition of pancreas lipids at experimental acute pancreatitis in rats

I. B. Pryvrotska1, T. M. Kuchmerovska2

1I. Нorbachevsky Ternopyl State Medical University, Ukraine;
2Palladin Institute of Biochemistry, National Academy of Science of Ukraine, Kyiv;
e-mail: irina.privrotska@yandex.uа

In an experimental model of acute pancreatitis (АP) in rats no alteration in leukocyte’s viability was found by flow cytometry as compared to control. After 1 day of AP production of reactive oxygen forms in granulocytes was increased more than 5 times, but after 3 days their level was decreased. Alterations of pro/antioxidant status and specific changes in the fatty acid composition in the pancreas were established. With the development of AP, the processes of lipids peroxidation were intensified while antioxidant system was altered, that was evidenced by inflammation in the pancreas. In these conditions, the increase of phospholipase A2 activity was accompanied by significant changes of fatty acid composition of the total lipids in the pancreas. This increased relative total content of saturated fatty acids, in particular myristic, palmitic and stearic acid increased, while the total content of polyunsaturated essential fatty acids ω-3 (linolenic, eicosapentaenoic, dokozapentayenoic, docosahexaenoic) decreased. The preparation containing ω-3 polyunsaturated fatty acids partially normalized the lipid and fatty acids composition as well as prooxidant-antioxidant system.

Keywords: , , , , ,


  1. Iaremchuk OZ, Posokhova KA. The liver and kidneys biochemical indices at the experimental pancreatitis in case of the administration of nitric oxide synthesis modulators and recombinant superoxide dismutase. Ukr Biokhim Zhurn. 2011 Jul-Sep;83(4):57-66. Ukrainian. PubMed
  2. Makarchuk VA, Ushakova HO, Krylova OO. The glutathione system in the blood of rats and morphological changes of the pancreas under experimental acute and chronic pancreatitis. Ukr Biokhim Zhurn. 2013 Jan-Feb;85(1):71-8. Ukrainian. PubMed, CrossRef
  3. Molteni R, Fabbri M, Bender JR, Pardi R. Pathophysiology of leukocyte-tissue interactions. Curr Opin Cell Biol. 2006 Oct;18(5):491-8. Review. PubMedCrossRef
  4. Voytenko GM, Gorchakova NO, Oleynik SA, Shevchenko LYu, Voytsitskiy VM, Marushko YuV, Kucherenko MYe. Experimental evaluation of pancrea protektive activity of “Polyphytol-1”. Dopovidi NAS Ukraine. 2002;(7):174-177.
  5. Abu-Hilal M, McPhail MJ, Marchand L, Johnson CD. Malondialdehyde and superoxide dismutase as potential markers of severity in acute pancreatitis.  J Pancreas (Online). 2006 Mar 9;7(2):185-92. PubMed
  6. Biradar S, Veeresh B. Protective effect of lawsone on L-Arginine induced acute pancreatitis in rats. Indian J Exp Biol. 2013 Mar;51(3):256-61. PubMed
  7. Lee S, Gura KM, Puder M. Omega-3 fatty acids and liver disease. Hepatology. 2007 Apr;45(4):841-5. PubMed, CrossRef
  8.  Kogteva GS, Bezuglov VV. Unsaturated fatty acids as endogenous bioregulators. Biochemistry (Mosc). 1998 Jan;63(1):4-12. Review. PubMed
  9. Gula N. M., Marhitych V. M. Fatty acids and their derivatives in pathological states. Kiev: Naukova dumka, 2009. 336 p. (In Ukrainian).
  10. Weylandt KH, Nadolny A, Kahlke L, Köhnke T, Schmöcker C, Wang J, Lauwers GY, Glickman JN, Kang JX. Reduction of inflammation and chronic tissue damage by omega-3 fatty acids in fat-1 transgenic mice with pancreatitis. Biochim Biophys Acta. 2008 Nov;1782(11):634-41. PubMed, PubMedCentralCrossRef
  11. Belenichev IF, Hubsky YuI, Levitsky EL. Antioxidant system of organism protection. Mod Probl Toxicol. 2002;3:24-31.
  12. Kubisch CH, Sans MD, Arumugam T, Ernst SA, Williams JA, Logsdon CD. Early activation of endoplasmic reticulum stress is associated with arginine-induced acute pancreatitis. Am J Physiol Gastrointest Liver Physiol. 2006 Aug;291(2):G238-45. PubMed, CrossRef
  13.  Pat. of Ukraine no.14794. Biologically active food supplement “Alfa+Omega”/Pokotylo O.S., no.20061181; publ. 10.06.2007, Official bulletin “Promyslova vlasnist” (Industrial property), No.8.
  14. Bass DA, Parce JW, Dechatelet LR, Szejda P, Seeds MC, Thomas M. Flow cytometric studies of oxidative product formation by neutrophils: a graded response to membrane stimulation. J Immunol. 1983 Apr;130(4):1910-7. PubMed
  15. Chevari S, Chaba I, Sekej I. Role of superoxide dismutase in cellular oxidative processes and method of its determination in biological materials. Lab Delo. 1985;(11):678-81. Russian. PubMed
  16.  Koroliuk MA, Ivanova LI, Mayorova IG, Tokarev VE. A method of determining catalase activity. Lab Delo. 1988;(1):16-9. Russian. PubMed
  17. Stalnaya ID. Modern methods in biochemistry. M.: Medicine, 1977. 391p.
  18. Andreeva LI, Kozhemiakin LA, Kishkun AA. Modification of the method of determining lipid peroxidation in a test using thiobarbituric acid. Lab Delo 1988;(11):41-3. Russian. PubMed
  19. Folch J, Lees M, Sloane Stanley GH. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497-509. PubMed
  20. Cert A, Moreda W, Pérez-Camino MC. Methods of preparation of fatty acid methyl esters (FAME). Statistical assessment of the precision characteristics from a collaborative trial. Grasas y Aceites. 2000;51;(6):447-456. CrossRef
  21. Tuzhilin SA, Saluen’ia AI. Method of determination of phospholipase A in blood serum. Lab Delo. 1975;(6):334-5. Russian. PubMed
  22. Kamyshnikov VS. Handbook of clinical and biochemical studies and laboratory diagnosis. Мoscow: MEDpress-inform, 2004. 920 p. (in Russian).
  23. Hardman J, Shields C, Schofield D, McMahon R, Redmond HP, Siriwardena AK. Intravenous antioxidant modulation of end-organ damage in L-arginine-induced experimental acute pancreatitis. Pancreatology. 2005;5(4-5):380-6. PubMed
  24. Awla D, Abdulla A, Zhang S, Roller J, Menger MD, Regnér S, Thorlacius H. Lymphocyte function antigen-1 regulates neutrophil recruitment and tissue damage in acute pancreatitis. Br J Pharmacol. 2011 May;163(2):413-23. PubMed, PubMedCentral, CrossRef
  25. Guzyk MM, Dyakun KO, Yanitska LV, Kuchmerovska ТМ.  Ukr Biokhim Zhurn. 2013 Jan-Feb;85(1):62-70. PubMedCrossRef
  26. Ruthenbürger M, Mayerle J, Brandt-Nedelev B, Jaroscakova I, Wartmann T, Hui DY, Halangk W, Lerch MM.  The role of pancreatic phospholipase A2 in experimental pancreatitis. Pancreas. 2006;33(4):493. CrossRef
  27.  Jaroscakova I, Brandt-Nedelev B, Wartmann T, Lippert H, Mayerle J, Hui DY, Halangk W, Lerch MM. Acute Pancreatitis. HPB (Oxford). 2005;7(1):59. PubMedCentralCrossRef
  28.  Taketo MM, Sonoshita M. Phospolipase A2 and apoptosis. Biochim Biophys Acta. 2002 Dec 30;1585(2-3):72-6. Review. PubMed
  29. Silonov SB, Donchenko HV, Morozova RP. Peculiarities of leukotrienes metabolism in vitamin E deficiency in rats. Ukr Biokhim Zhurn. 2008 Jan-Feb;80(1):26-32. Ukrainian. PubMed
  30. Kilian M, Gregor JI, Heukamp I, Wagner C, Walz MK, Schimke I, Kristiansen G, Wenger FA. Early inhibition of prostaglandin synthesis by n-3 fatty acids determinates histologic severity of necrotizing pancreatitis. Pancreas. 2009 May;38(4):436-41. PubMed, CrossRef
  31. Panek J, Sztefko K, Drozdz W. Composition of free fatty acid and triglyceride fractions in human necrotic pancreatic tissue. Med Sci Monit. 2001 Sep-Oct;7(5):894-8. PubMed
  32. Simopoulos AP. The importance of the omega-6/omega-3 fatty acid ratio in cardiovascular disease and other chronic diseases. Exp Biol Med (Maywood). 2008 Jun;233(6):674-88. Review. PubMedCrossRef
  33. Foitzik T, Eibl G, Schneider P, Wenger FA, Jacobi CA, Buhr HJ. Omega-3 fatty acid supplementation increases anti-inflammatory cytokines and attenuates systemic disease sequelae in experimental pancreatitis. JPEN J Parenter Enteral Nutr. 2002 Nov-Dec;26(6):351-6.PubMed
  34. Shysh АM, Kaplinskiy SP, Nagibin VS, Dosenko VE, Moibenko АА. Investigation of anti-inflammatory mechanisms of omega-3 polyunsaturated fatty acids. Pathologia. 2011;8(3):74-77.

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