Ukr.Biochem.J. 2012; Volume 84, Issue 4, Jul-Aug, pp. 32-40

Influence of chemically different antiviral substances on the expression of IFNα, PKR, OAS1a and RNAse L genes

О. O. Dragushchenko1,2, I. I. Minia1, T. A. Poliezhaeva1,2, D. B. Starosyla3,
I. S. Karpova1, M. Yu. Obolenska1, S. L. Rybalko3

1Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv;
2Taras Shevchenko Kyiv National University, Institute of Biology, Ukraine;
3State Institution L. V. Gromashevskiy Institute of Epidemiology and Infectious Diseases,
National Academy of Medical Sciences of Ukraine, Kyiv;

The aim of the study was to determine the ability of several chemically different antiviral substances to induce the expression of interferon α (IFNα), PKR, OAS1a and RNAse L genes in the rat liver. The investigated substances included Amizon, Altabor and Proteflasid, which are already used in practical medicine, and 3′,7-dimethylquercetin extracted from Proteflasid, the mixture of synthesized trimethyl- and tetramethylquercetin and Sialospecific lectin from persimmon, which are at the stage of preclinical trial and experimental research respectively.
The content of corresponding mRNAs in total RNA was detected with the help of reverse transcription and polymerase chain reaction in real time. The results have shown that all investigated substances induce the expression of genes IFNα, PKR, OAS and RNAse L in specific manner. The combination of 3′,7-dimethylquercetin + lectin from persimmon had the highest stimulating effect exceeding the effect of each component of the mixture and the influence of Heberon (recombinant IFNα2b) and PolyI-polyC as the standard inducers of IFNα and its target genes.
The ability of all substances to specifically induce the expression of IFNα and its target genes, the absence of correlation between the levels of IFNα and its target genes expression as well as between target genes themselves indicate that the mechanism of antiviral activity of the investigated substances is connected not only with up-regulation of IFNα and potential IFNα mediated effects.

Keywords: , , , , , , ,

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