Ukr.Biochem.J. 2016; Volume 88, Issue 4, Jul-Aug, pp. 48-56

doi: https://doi.org/10.15407/ubj88.04.048

Monooxygenase system in Guerin’s carcinoma of rats under conditions of ω-3 polyunsaturated fatty acids administration

01.09.2016   Uncategorized   No comments

M. M. Marchenko, O. V. Ketsa, I. O. Shmarakov, K. H. Abutnaritsa

Fedkovich Chernovtsy National University, Ukraine;
e-mail: ketsa80@mail.ru

The aim of the study was to determine the variations of function in components of monooxygenase system (MOS) of rat Guerin’s carcinoma  under ω-3 polyunsaturated fatty acids (PUFAs) administration. The activity of Guerin’s carcinoma microsomal NADH-cytochrome b5 reductase, the content and the rate of cytochrome b5 oxidation-reduction, the content and the rate of cytochrome Р450 oxidation-reduction have been investigated in rats with tumor under conditions of ω-3 PUFAs administration. ω-3 PUFAs supplementation before and after transplantation of Guerin’s carcinoma resulted in the increase of NADH-cytochrome b5 reductase activity and decrease of cytochrome b5 level in the Guerin’s carcinoma microsomal fraction in the logarithmic phases of carcinogenesis as compared to the tumor-bearing rats. Increased activity of NADH-cytochrome b5 reductase facilitates higher electron flow in redox-chain of MOS. Under decreased cytochrome b5 levels the electrons are transferred to oxygen, which leads to heightened generation of superoxide (O2•-) in comparison to control. It was shown, that the decrease of cytochrome P450 level in the Guerin’s carcinoma microsomal fraction in the logarithmic phases of oncogenesis under ω-3 PUFAs administration may be associated with its transition into an inactive form – cytochrome P420. This decrease in cytochrome P450 coincides with increased generation of superoxide by MOS oxygenase chain.

Keywords: , , , , , ,

References:

  1. Serini S, Fasano E, Celleno L, Cittadini A, Calviello G. Potential of long-chain n-3 polyunsaturated fatty acids in melanoma prevention. Nutr Rev. 2014 Apr;72(4):255-66.  Review. PubMed, CrossRef
  2. Wang S, Wu J, Suburu J, Gu Z, Cai J, Axanova LS, Cramer SD, Thomas MJ, Perry DL, Edwards IJ, Mucci LA, Sinnott JA, Loda MF, Sui G, Berquin IM, Chen YQ. Effect of dietary polyunsaturated fatty acids on castration-resistant Pten-null prostate cancer. Carcinogenesis. 2012 Feb;33(2):404-12. PubMed, PubMedCentral, CrossRef
  3. Berquin IM, Edwards IJ, Chen YQ. Multi-targeted therapy of cancer by omega-3 fatty acids. Cancer Lett. 2008 Oct 8;269(2):363-77. Review. PubMed, PubMedCentral, CrossRef
  4. Davidson J, Rotondo D, Rizzo MT, Leaver HA. Therapeutic implications of disorders of cell death signalling: membranes, micro-environment, and eicosanoid and docosanoid metabolism. Br J Pharmacol. 2012 Jun;166(4):1193-210. PubMed, PubMedCentral, CrossRef
  5. Greene ER, Huang S, Serhan CN, Panigrahy D. Regulation of inflammation in cancer by eicosanoids. Prostaglandins Other Lipid Mediat. 2011 Nov;96(1-4):27-36. Review. PubMed, PubMedCentral, CrossRef
  6. Gladine C, Mazur A. Nutrigenomic effects of omega-3 fatty acids. Lipid Technology. 2014;26(10): 227-229. CrossRef
  7. Recchiuti A, Serhan CN. Pro-Resolving Lipid Mediators (SPMs) and Their Actions in Regulating miRNA in Novel Resolution Circuits in Inflammation. Front Immunol. 2012 Oct 22;3:298. PubMed, PubMedCentral, CrossRef
  8. Shmarakov IA, Katan NV. The Induction of Guerin’s carcinoma cytochrome P450 hydroxylase activity by retinoids. Biochemistry (Mosc) Suppl Ser B Biomed Chem. 2011 Nov; 5(4)369-375. PubMed, CrossRef
  9. Schenkman JB, Cinti DL. Preparation of microsomes with calcium. Methods Enzymol. 1978;52:83-9. PubMed, CrossRef
  10. Orekhovich VN. Ed., Moden method in biochemistry, Moscow: Meditsina. 386 p. (In Russian).
  11. Mokhosoev IM, Kuznetsova GP, Al’terman MA, Bachmanova GI, Archakov AI. Inactivation of sodium dithionite reduced cytochromes P-450 of different origins. Biokhimiia. 1987 Oct;52(10):1649-58. (In Russian). PubMed
  12. Kostenko VO, Tsebrzhins’kii OI. Production of superoxide anion radical and nitric oxide in renal tissues sutured with different surgical suture material. Fiziol Zh. 2000;46(5):56-62. (In Ukrainian). PubMed
  13. Lowry OH, Rosenbrough MJ, Farr AL, Rendal RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265-75. PubMed
  14. Marchenko MM, Ketsa O., Veliky NN. Biochemical transformation of xenobiotics in the body: monograph. Chernivtsi: Chernivtsi University, 2011. 280 p. (In Ukrainian).
  15. Marchenko MM, Kopylchuk HP, Ketsa OV, Shmarakov IO. Effect of liposomal antitumor preparation 5-(5′,6′-benzocoumarin-3′)-methylaminouracil hydrobromide on cytochrome P-450 in the microsomal liver fraction of tumor bearing rats. Ukr Biokhim Zhurn. 2006 Nov-Dec;78(6):86-92. (In Ukrainian). PubMed
  16. Kaur R, Arora S. Interactions of betulinic acid with xenobiotic metabolizing and antioxidative enzymes in DMBA-treated Sprague Dawley female rats. Free Radic Biol Med. 2013 Dec;65:131-42. PubMed, CrossRef
  17. Guengerich FP. Cytochrome p450 and chemical toxicology. Chem Res Toxicol. 2008 Jan;21(1):70-83. Review. PubMed, CrossRef
Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License.