Ukr.Biochem.J. 2019; Volume 91, Issue 3, May-Jun, pp. 56-64

doi: https://doi.org/10.15407/ubj91.03.056

Kinetic properties of Na(+),K(+)-АТРase of spermatozoa from fertile and infertile men under effect of calix[4]arene C-107

R. V. Fafula, O. I. Meskalo, A. S. Besedina,
Io. A. Nakonechnyi, D. Z. Vorobets, Z. D. Vorobets

Danylo Halytsky Lviv National Medical University, Ukraine;
e-mail: kaf_medicalbiology@meduniv.lviv.ua; roman_fafula@ukr.net

Received: 12 November 2018; Accepted: 14 March 2019

The calix[4]arene C-107 (5,17-diamino(2-pyridyl)methylphosphono-11,23-di-tret-butyl-26,28-dihydro­xy-25,27-dipropoxy-calix[4]arene) effects on the kinetic properties of Na+,K+-ATPase in spermatozoa of fertile (normozoospermia) and infertility men (oligozoospermia, and asthenozoospermia) were studied. It was shown that in spermatozoa of healthy men calix[4]arene С-107 inhibited Na+,K+-ATPase activity and decreased the maximum reaction rate of ATP hydrolase reaction without affecting the coefficient of (half-) activation by ATP and Hill coefficient nH. In оligo- and asthenozoospermic samples of spermatozoa almost a 2-fold decrease of cooperativity coefficient nH of ATPase inhibition with calyx[4]aren C-107 was observed. In normozoospermic samples of spermatozoa the KMgCl2  for Na+,K+-ATPase was decreased at calix[4]arene C-107 high concentrations (≥50 nM) in the incubation medium in contrast to oligozoospermic samples of spermatozoa where KMgCl2 was increased only at high calix[4]arene C-107concentration (100 nM). The increase of the KMgCl2 in the entire range of investigated calix[4]arene concentrations and the decrease of cooperativity coefficient nH of MgCl2 activating effect were detected in asthenozoospermic samples of Na+­,K+-ATPase.

Keywords: , , ,


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