Tag Archives: lipid peroxidation
Prooxidant and antioxidant processes in the liver homogenate of healthy and tumor-bearing mice under the action of thiazole derivatives
Ya. R. Shalai1*, M. V. Popovych1, S. M. Mandzynets1,
V. P. Hreniukh1, N. S. Finiuk1,2, A. M. Babsky1
1Ivan Franko National University of Lviv, Ukraine;
2Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv;
*e-mail: Yaryna.Shalay@lnu.edu.ua
Received: 12 October 2021; Accepted: 17 May 2021
Thiazole derivatives were shown to have toxic effects in vitro on cancer cells of different origin and can be considered as potentially antineoplastic, but their effect on the normal tissues needs to be studied. In this research the newly synthesized thiazole derivatives of N-(5-benzyl-1,3-thiazol-2-yl)-3,5-dimethyl-1-benzo-furan-2-carboxamide (BF1) and 8-methyl-2-Me-7-[trifluoromethyl-phenylmethyl]-pyrazolo [4,3-e] [1,3] thiazolo [3,2-a] pyrimidin-4 (2H)-one (PP2) were used and their effect on the pro- and antioxidant processes after adding in a 1, 10 and 50 μM concentrations to the liver homogenate of healthy and NK/Ly lymphoma-bearing mice was estimated. The level of superoxide radical and TBA-active products as well as catalase, SOD and glutathione peroxidase activity were measured. It was shown that superoxide radical and TBA-active products level and catalase activity were significantly higher in the liver of tumor-bearing mice than in the liver of the healthy mice. Neither BF1 no PP2 influenced the studied indices in the liver homogenate of healthy and tumor-bearing animals with the exception that PP2 significantly reduced the level of TBA-positive products in both cases. The data obtained showed that the studied thiazole derivatives did not cause severe liver toxicity in both healthy and tumor-bearing mice.
Quercetin and histamine effects on free radical reactions in rat erythrocytes
N. P. Harasym*, M. Y. Booklyv, A. R. Zyn, S. M. Mandzynets,
A. O. Bezkorovainy, D. I. Sanahursky
Ivan Franko National University of Lviv, Faculty of Biology, Departments of Biophysics and Bioinformatics, Ukraine;
*e-mail: garasymnataly@gmail.com
Received: 12 June 2020; Accepted: 17 December 2020
The effects of quercetin and histamine separately or in combination on the free radical state of rat erythrocytes were estimated in vitro. Quercetin (0.1; 0.5; 3.0; 5.0 mM) or histamine (0.01; 10.0 μM) were added to whole blood separately or in combination. The content of hydroperoxides, TBA-active products and carbonyl groups of proteins in erythrocytes after hemolysis was determined. The greatest influence of quercetin and histamine on erythrocytes state indicators was revealed under their combined action, when the level of TBA-active products and the content of carbonyl groups of proteins were found to be increased substantially.
Free radical oxidation in liver mitochondria of tumor-bearing rats and its correction by essential lipophilic nutrients
O. V. Ketsa, M. M. Marchenko
Fedkovich Chernivtsy National University, Ukraine;
e-mail: o.ketsa@chnu.edu.ua
Received: 21 May 2019; Accepted: 29 November 2019
The role of free radical oxidation in the increase of mitochondrial membranes permeability in organs which are not involved in oncogenesis and the development of the methods for preventing mitochondria dysfunction remain topical problems. In this work, the interconnection of lipid peroxidation (LPO) in liver mitochondrial fraction with the processes of mitochondrial swelling and cytochrome с release to the cytosol under separate and combined administration of ω-3 polyunsaturated fatty acids (PUFAs) and retinol acetate (vitamin A acetate) to rats with transplanted Guerin’s carcinoma was studied. During the intensive tumor growth (14 days) the increase of superoxide radical generation and the content of primary (triene conjugates, TC), secondary (ketodienes and coupled trienes, CD+CT) and terminal (Schiff bases) lipid peroxidation products in the mitochondrial fraction of tumor-bearing rats was detected, which contributed to the mitochondrial swelling and cytochrome с release to the cytosol. Separate administration of ω-3 PUFAs to tumor-bearing rats decreased both free radical processes in mitochondrial fraction and mitochondrial swelling. Separate administration of retinol acetate in a high dose (3000 IU/kg of body weight) intensified free radical processes in the mitochondrial fraction of tumor-bearing rats, while administration of retinol acetate in a physiological dose (30 IU/kg of body weight) did not lead to changes compared to tumor-bearing rats that did not receive the drug. The prooxidant effects of retinoid were partially eliminated in the case of combined administration with ω-3 PUFA.
Phenylalanine ammonia-lyase activity and content of flavonoid compounds in wheat seedlings at the action of hypothermia and hydrogen sulfide donor
Yu. E. Kolupaev1,2, E. I. Horielova1, T. O. Yastreb1, Yu. V. Popov3, N. I. Ryabchun3
1Dokuchaev Kharkiv National Agrarian University, Ukraine;
e-mail: plant_biology@ukr.net;
2Karazin Kharkiv National University, Ukraine;
3Yuryev Рlant Production Institute, National Academy of Agrarian Sciences of Ukraine, Kharkiv
At present hydrogen sulfide (H2S) is considered as one of the signal mediators in plant cells. However, its role in formation of plant resistance to low temperatures and, in particular, in regulation of secondary metabolism under stress conditions remains poorly understood. The influence of H2S donor sodium hydrosulfide (NaHS) on phenylalanine ammonia-lyase (PAL) activity and content of flavonoids in wheat seedlings at normal temperature (21 °C) and under cold hardening conditions (7 days at 3 °C) was studied. After 2 days of the hardening temperature, a transient increase in PAL activity was noted. Also, activity of the enzyme was increased by treatment of plants with 0.1 or 0.5 mM NaHS under normal temperature conditions and especially at the background of cold hardening. By themselves, the cold hardening and the action of H2S donor caused an increase in total content of flavonoids and amount of anthocyanins. With the combination of hypothermia and treatment of seedlings with NaHS, this effect enlarged and the total content of flavonoids increased by 3.8, and anthocyanins increased by 1.8 times in comparison to the control. Treatment with the H2S donor caused a decrease in content of the lipid peroxidation product malonic dialdehyde in seedlings after the action of hardening temperature, and especially after their freezing at –5 °C. Also, under the influence of NaHS, survival of hardened and unhardened seedlings after cryostress increased. It was concluded that one of the mechanisms of the positive influence of the H2S donor on resistance of wheat seedlings to hypothermia is the PAL-dependent accumulation of flavonoid compounds, which have a high antioxidant activity, and a decrease in effects of secondary oxidative stress.
Effect of sodium nitroprusside and S-nitrosoglutathione on pigment content and antioxidant system of tocopherol-deficient plants of Arabidopsis thaliana
N. M. Semchuk, Yu. V. Vasylyk, O. I. Kubrak, V. I. Lushchak
Vassyl Stefanyk Precarpathian National University, Ivano-Frankivsk, Ukraine;
e-mail: lushchak@pu.if.ua
Sodium nitroprusside (SNP) and S-nitrosoglutathione (GSNO) were used as a source of exogenous nitric oxide (NO) to investigate their effects on biochemical parameters and antioxidant enzyme response in leaves of wild type Columbia and tocopherol-deficient vte4 and vte1 mutant lines of Arabidopsis thaliana plants and possible tocopherol involvement in regulation of antioxidant response under NO-induced stress. SNP enhanced the activity of the enzymes, that scavenge hydrogen peroxide in leaves of all studied lines, and increased glutathione reductase and glutathione-S-transferase activity there. In addition, it decreased the intensity of lipid peroxidation in vte1 mutant line leaves. At the same time, GSNO increased the levels of protein carbonyls and inactivated enzymes ascorbate peroxidase, guaiacol peroxidase and dehydroascorbate reductase in almost all investigated plant lines. In contrast to wild type, GSNO increased superoxide dismutase activity and decreased catalase activity and chlorophyll a/b ratio in the leaves of two mutant lines. It can be assumed that tocopherols in some way are responsible for plant protection against NO-induced stress. However the mechanisms of this protection remain unknown.
Changes in oxidative stress intensity in blood of tumor-bearing rats following different modes of administration of rhenium-platinum system
K. L. Shamelashvili1, N. I. Shtemenko2, І. V. Leus3, S. O. Babiy4, O. V. Shtemenko5
1SE “Dnipropetrovsk Medical Academy” of Health Ministry of Ukraine;
2Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
3University of Oklahoma, Oklahoma-city, USA;
4Institute of Gastroenterology, National Academy of Medical Sciences
of Ukraine, Dnipropetrovsk;
5Ukrainian State University of Chemical Technology, Dnipropetrovsk;
e-mail: shamelashvili@rambler.ru
Effects of the different modes of administration of dichlorotetra-μ-isobutyratodirhenium(ІІІ) – І – (in water solution, liposomes, nanoliposomes and together with cisplatin – in the rhenium-platinum system) on the intensity of lipid peroxidation (LP) in blood plasma and the activity of the erythrocyte antioxidant enzymes were investigated on the model of tumor growth. A decrease in the concentration of TBA-active substances caused by dirhenium compounds was shown to be independent of the administration mode and the extent of the tumor growth inhibition. I was four-times more effective in inhibition of the LP burst than any known antioxidant. I induced the increasing activity of erythrocyte superoxide dismutase and decreasing activity of catalase. In vitro experiments with native superoxide dismutase, the interaction of І with following activation of the active center of the enzyme was confirmed and the superoxide dismutase activity of І was shown, that may contribute to the enhancement of the enzyme activity in vivo. The cluster rhenium compounds may be promising nontoxic potent antioxidants capable of deactivating superoxide radicals.
Loach embryos prooxidant-antioxidant status under the influence of amide derivatives of 1,4-naphthoquinone
A. O. Bezkorovaynyj1,2, A. R. Zyn2, N. M. Harasym1, J. T. Len2,3, O. M. Figurka3, D. I. Sanagursky1
1Ivan Franko National University of Lviv, Ukraine;
2Lviv Expert Center of Scientific Researches Ministry of Internal Affairs of Ukraine;
3National University “Lviv Polytechnicа”, Ukraine;
e-mail: andriy.bezkorovajnyj@gmail.com
The mechanisms of disorders in cell functions induced by 1,4-naphthoquinone amide derivatives are not clarified yet. The article is dedicated to the study of features of these substances influence on loach Misgurnus fossilis L. embryos pro/antioxidant homeostasis during early embryogenesis. The aim of this work was to study the effect of 2-chloro-3-hydroxy-1,4-naphthoquinone, 2-chloro-3-(3-oxo-3-(piperidine-1-yl)propylamine)-1,4-naphthoquinone (FO-1), 2-chloro-3-(3-(morpholine-4-yl)-3-oxopropylamine)-1,4-naphthoquinone (FO-2 at concentrations of 10-3, 10-5, 10-7 M on the content of TBA-reactive substances (a byproduct of lipid peroxidation) and the activities of superoxide dismutase and catalase in loach embryos. It was established that 1,4-naphthoquinone amide derivatives and 2-chloro-3-hydroxy-1,4-naphthoquinone decreased the content of lipid peroxidation products in embryo cells in a dose-dependent manner. The investigated compounds cause an increase in superoxide dismutase and catalase activities compared with the control value. The results of the two-factor ANOVA test indicate that 2-chloro-3-hydroxy-1,4-naphthoquinone and 1,4-naphthoquinone amide derivatives (FO-1, FO-2) have predominant influence on the TBA-reactive substances content and superoxide dismutase activity. However, the time of loach embryos development has a more pronounced effect on catalase activity than the studied 1,4-naphthoquinone derivatives.
Tissue specificity of lipid peroxidation under emotional stress in rats
K. O. Menabde, G. M. Burjanadze, M. V. Сhachua,
Z. T. Kuchukashvili, N. I. Koshoridze
Iv. Javakhishvili Tbilisi State University, Georgia;
e-mail: ketimenabde@yahoo.com
The intensity of lipid peroxidation and activity of antioxidant system enzymes in the blood plasma, brain and cardial muscle of laboratory rats under 40 days of isolation and violation of diurnal cycle was studied. The obtained data show that on the background of concentration changes in NO changes also take place in the intensity of lipid peroxidation process, indicated by changes in the concentration of TBA-active products and diene conjugates.
The changes taking place in the activity of superoxidedismutase, catalase, succinatdehydrogenase, creatine kinase and aldolase under stress were studied.
The resulting data show that isolation of animals and violation of diurnal cycle are the factors causing a significant reduction in the energy metabolism in the brain and heart tissue cells and resulting in oxidative stress that, in its turn, may become the reason for development of toxic radicals.
Furthermore, prolonged stress may result in irreversible processes that are considered to be the reasons for significant pathologies of the cardiovascular system.
Peculiarities of antioxidant defense system organization of the black sea mollusks Mytilus galloprovincialis Lam. and Anadara inaequivalvis Br.
O. L. Gostyukhina, I. V. Golovina
O. O. Kovalevsky Institute of Biology of the Southern Seas,
National Academy of Sciences of Ukraine, Sevastopol;
e-mail: gostolga@yandex.ru
Antioxidant (AO) system and lipid peroxidation (LP) in tissues of two species of the Black Sea bivalve mollusks Mytilus galloprovincialis and Anadara inaequivalvis were investigated. The activity of superoxide dismutase (SOD, 1.15.1.1), catalase (1.11.1.6), glutathione peroxidase (GP, 1.11.1.9), glutathione reductase (GR, 1.6.4.2), concentrations of reduced glutathione (GSH) and TBA-reactive products were determined in the foot, hepatopancreas and gills of mature mollusks. The characteristics of AO complex and LP products connected with tissue and species specificity of mollusks were found. Hepatopancreas of mussels has been found to have higher values of all characteristics investigated, except GP. The gills and the foot of anadara have been found to be involved in AO defense along with hepatopancreas: maximum activity of GR, catalase and SOD was found in the gills and the highest activity of GP and maximum level of GSH was observed in the foot. Anadara has been shown to have higher antioxidant potential and lower level of oxidative stress because the LP intensity in all tissues examined of the hemoglobin-containing mollusk was twice lower in comparison with the mussel.