A. I. Archakov¹, V. G. Zgoda¹, A. T. Kopylov¹, S. N. Naryzhny^1,2,
A. L. Chernobrovkin¹, E. A. Ponomarenko¹, A. V. Lisitsa¹

¹V. N. Orekhovich Scientific-Research Institute  of Biomedical Chemistry, Russian Academy of Medical Sciences;
²Petersburg Nuclear Physics Institute at National  Research Centre Kurchatov Institute

The international project Human Proteome (PHP), being a logical continuation of the project Human Genome, was started on September 23, 2010. In correspondence with the genocentric approach, the PHP aim is to prepare a catalogue of all human proteins and to decipher a network of their interactions. The PHP implementation difficulties arise because the research subject itself – proteome – is much more complicated than genome. The major problem is the insufficient sensitivity of proteome methods that does not allow detecting low- and ultralow-copy proteins. Bad reproducibility of proteome methods and the lack of so-called “gold standard” is the second major complicacy in PHP implementation. The third problem is the dynamic character of proteome, its instabili­ty in time. The paper deals with possible variants of overcoming these complicacies, preventing from successful implementation of PHP.