Tag Archives: sarcoplasmic reticulum
The inhibitory influence of calix[4]arene of C-90 on the activity of Ca(2+),Mg(2+)-ATPases in plasma membrane and sarcoplasmic reticulum in myometrium сells
T. O. Veklich
Palladin Institute of Biochemistry,National Academy of Sciences of Ukraine, Kyiv;
e-mail: veklich@biochem.kiev.ua
Our study on the plasma membrane vesicles and myometrium cells treated with 0.1% digitonin showed that inhibitory effect of calix[4]arene C-90 (5,11,17,23-tetra(trifluoro)methyl(phenylsulphonylimino)-methylamino-25,26,27,28-tetrapropoxy-calix[4]arene) on the plasma membrane Ca2+,Mg2+-ATPase was more significant than on the Ca2+,Mg2+-ATPase in sarcoplasmic reticulum (the inhibition coefficient I0.5 values were 20.2 ± 0.5 µM and 57.0 ± 1.4 µM for the plasma membrane Ca2+,Mg2+-ATPase and Ca2+,Mg2+-ATPase in sarcoplasmic reticulum, respectively (n = 5)). Inhibition kinetics of calix[4]arene C-90 effect on the Ca2+,Mg2+-ATPase activities in plasma membrane and sarcoplasmic reticulum were studied. This substance inhibited both pumps as complete noncompetitive inhibitor. Calix[4]arene C-90 caused the increase of intracellular Ca2+ concentration and decrease of hydrodynamic diameter in smooth muscle cells similar to the action of uterotonic drug oxytocin.
Laureates of the Palladin Prize of the National Academy of Sciences of Ukraine (1991–1992)
V. M. Danilova, R. P. Vynogradova
Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
e-mail: valdan@biochem.kiev.ua
The article presents biographical information and analysis of scientific activity of laureates of the Palladin Prize of NAS of Ukraine G. V. Donchenko (1991) and S. O. Kosterin (1992) who were awarded for the monographs “Biochemistry of ubiquinone” and “Calcium transport in smooth muscles”, respectively. These monographs summarized the results of the original researches and the data of the world scientific literature.