Ukr.Biochem.J. 2012; Volume 84, Issue 1, Jan-Feb, pp. 53-59
Antioxidant properties of proteins after freezing-thawing
S. L. Rozanova, E. D. Rozanova, O. A. Nardid
Institute for Problems of Cryobiology and Cryomedicine,
National Academy of Sciences of Ukraine, Kharkiv;
e-mail: sv.rosanova@gmail.com
Experimental data are presented which were obtained under comparative evaluation of influence of different freezing-thawing conditions on antioxidant properties of isolated proteins: human serum albumin, cytochrome с from the horse heart and glucose oxidase from Aspergillus niger. The observed protein antioxidant activity alterations are assumed to be a result of protein conformational changes. The character of freezing-thawing influence on the protein antioxidant activity depends on the molecular structure and cooling conditions.
Keywords: albumin, antioxidant activity, cytochrome с, freezing-thawing, glucoseoxidase
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