Ukr.Biochem.J. 2011; Volume 83, Issue 1, Jan-Feb, pp. 83-86
Solid phase extraction of BSA
V. A. Khalaf1, О. A. Vasylchenko2, V. M. Levchyk1, G. M. Zaitseva1
1Taras Shevchenko Kyiv National University, Ukraine;
e-mail: khalaf@univ.kiev.ua;
2National Aviation University, Kyiv, Ukraine
Silica with immobilized polyoxyethyleneisooctylphenol (SiO2–TX) was investigated as an adsorbent for solid phase extraction of bovine serum albumin (BSA). It was shown that efficient BSA extraction (up to 96%) takes place on SiO2–TX from water solution in the form of its ionic associates with cationic (at рН = 8) and anionic (at рН = 1.5) surfactants.
Keywords: bovine serum albumin, ionic associate, solid phase extraction, surfactants
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