O. Omelchuk¹, S. Prylutska², D. Nozdrenko³,
O. Motuziuk^1,3, I. Vareniuk³, K. Bogutska³,
O. Vygovska⁴, А. Zholos³, Yu. Prylutskyy³*

¹Lesya Ukrainka Volyn National University, Lutsk, Ukraine;
²National University of Life and Environmental Science of Ukraine, Kyiv, Ukraine;
³Taras Shevchenko National University of Kyiv, Ukraine;
*e-mail: prylut@ukr.net;
⁴Bogomolets National Medical University, Kyiv, Ukraine

Received: 14 August 2023; Revised: 18 September 2023;
Accepted: 27 October 2023; Available on-line: 06 November 2023

Rhabdomyolysis, as an acute stage of myopathy is known to be associated with the accumulation of muscle breakdown products, acute renal failure and oxidative stress. The goal of the study was to evaluate the effect of C₆₀ fullerene as an antioxidant on kidney damage in the model of glycerol-induced rhabdomyolysis in rats. The study was conducted on male Wistar rats, divided into the following experimental groups: control animals, animals intramuscularly injected with glycerol in a doses of 5, 10 and 15 mg/kg and those intraperitoneally injected daily with C₆₀ fullerene aqueous solution (C₆₀FAS) in a dose of 1 or 2 mg/kg at 48 h after glycerol administration­. Monitoring of the biochemical and morphological indicators was carried out on 3^rd, 6^th and 9^th days of the experiment. A close correlation between the acute renal damage severity, increased creatinine and urea level, superoxide dismutase (SOD) and catalase (CAT) activity in the blood of rats was observed. It was shown that in rats which received 2 mg/kg of C₆₀FAS the renal glomeruli size and necrosis manifestations were attenuated, whereas SOD and CAT activity in the blood was significantly decreased. The results obtained may be useful for developing approaches to the treatment of pathological conditions of the muscular system caused by rhabdomyolysis and associated oxidative stress.