Tag Archives: angiotensin converting enzyme

Intensity of oxidative stress and activity of angiotensin converting enzyme in blood of patients with uncomplicated pyelonephritis

L. V. Korol, L. Ya. Mygal, N. M. Stepanova

State Institution Institute of Nephrology, National Academy
of Medical Sciences of Ukraine, Kyiv;
e-mail: lesyakorol@meta.ua

The purpose of this work was to study the correlation between oxidative stress (OS) marker and angiotensin converting enzyme (ACE) activity in patients with chronic kidney disease stages I-II (uncomplicated pyelonephritis). The 32 patients with uncomplicated pyelonephritis and 30 healthy volunteers (women, age – 18-40 years) were involved in this study. The content of thiobarbituric acid reactive products, protein carbonyl groups, ceruloplasmin, transferrin, the SH-groups, the total peroxidase activity of erythrocytes were determined by colorimetric method. OS index was calculated. To evaluate the functional state of the renal parenchyma the activity of tubular lysosome enzymes a total β-N-acetylhexosaminidase and β-galactosidase were determined in urine. The correlation analysis between activities of ACE and OS was performed. The ACE increased activity was shown on the background of violations of pro/antioxidant balance. The correlation analysis confirmed the presence of a moderate relationship between ACE activity and the majority of the studied parameters. Thus, an increase in ACE activity, intensification of oxidative processes, decrease of antioxidant defense contributes to the development of local OS, as well as the development of dysfunction in renal tubular system (according to the increased activity of renal specific enzymes in the urine).