Ukr.Biochem.J. 2017; Volume 89, Issue 2, Mar-Apr, pp. 99-105


Intensity of oxidative stress and activity of angiotensin converting enzyme in blood of patients with uncomplicated pyelonephritis

L. V. Korol, L. Ya. Mygal, N. M. Stepanova

State Institution Institute of Nephrology, National Academy
of Medical Sciences of Ukraine, Kyiv;

The purpose of this work was to study the correlation between oxidative stress (OS) marker and angiotensin converting enzyme (ACE) activity in patients with chronic kidney disease stages I-II (uncomplicated pyelonephritis). The 32 patients with uncomplicated pyelonephritis and 30 healthy volunteers (women, age – 18-40 years) were involved in this study. The content of thiobarbituric acid reactive products, protein carbonyl groups, ceruloplasmin, transferrin, the SH-groups, the total peroxidase activity of erythrocytes were determined by colorimetric method. OS index was calculated. To evaluate the functional state of the renal parenchyma the activity of tubular lysosome enzymes a total β-N-acetylhexosaminidase and β-galactosidase were determined in urine. The correlation analysis between activities of ACE and OS was performed. The ACE increased activity was shown on the background of violations of pro/antioxidant balance. The correlation analysis confirmed the presence of a moderate relationship between ACE activity and the majority of the studied parameters. Thus, an increase in ACE activity, intensification of oxidative processes, decrease of antioxidant defense contributes to the development of local OS, as well as the development of dysfunction in renal tubular system (according to the increased activity of renal specific enzymes in the urine).

Keywords: , , , ,


  1. Montezano AC, Dulak-Lis M, Tsiropoulou S, Harvey A, Briones AM, Touyz RM. Oxidative stress and human hypertension: vascular mechanisms, biomarkers, and novel therapies. Can J Cardiol. 2015 May;31(5):631-41. PubMed, CrossRef
  2. Putri AY, Thaha M. Role of oxidative stress on chronic kidney disease progression. Acta Med Indones. 2014 Jul;46(3):244-52. Review. PubMed
  3. Touyz RM. Reactive oxygen species and angiotensin II signaling in vascular cells — implications in cardiovascular disease. Braz J Med Biol Res. 2004 Aug;37(8):1263-73. Review. PubMed, CrossRef
  4. Montezano AC, Touyz RM. Oxidative stress, Noxs, and hypertension: experimental evidence and clinical controversies. Ann Med. 2012 Jun;44 Suppl 1:S2-16.  PubMed, CrossRef
  5. Arellano-Mendoza MG, Vargas-Robles H, Del Valle-Mondragon L, Rios A, Escalante B. Prevention of renal injury and endothelial dysfunction by chronic L-arginine and antioxidant treatment. Ren Fail. 2011;33(1):47-53.  PubMed, CrossRef
  6. Agarwal R. Proinflammatory effects of oxidative stress in chronic kidney disease: role of additional angiotensin II blockade. Am J Physiol Renal Physiol. 2003 Apr;284(4):F863-9.  PubMed, CrossRef
  7. Bruder-Nascimento T, Chinnasamy P, Riascos-Bernal DF, Cau SB, Callera GE, Touyz RM, Tostes RC, Sibinga NE. Angiotensin II induces Fat1 expression/activation and vascular smooth muscle cell migration via Nox1-dependent reactive oxygen species generation. J Mol Cell Cardiol. 2014 Jan;66:18-26. PubMed, PubMedCentral, CrossRef
  8. Kanzelmeyer NK, Pape L, Chobanyan-Jürgens K, Tsikas D, Hartmann H, Fuchs AJ, Vaske B, Das AM, Haubitz M, Jordan J, Lücke T. L-arginine/NO pathway is altered in children with haemolytic-uraemic syndrome (HUS). Oxid Med Cell Longev. 2014;2014:203512. PubMed, PubMedCentral, CrossRef
  9. Lorin J, Zeller M, Guilland JC, Cottin Y, Vergely C, Rochette L. Arginine and nitric oxide synthase: regulatory mechanisms and cardiovascular aspects. Mol Nutr Food Res. 2014 Jan;58(1):101-16.  PubMed, CrossRef
  10. Cirillo P, Sautin YY, Kanellis J, Kang DH, Gesualdo L, Nakagawa T, Johnson RJ. Systemic inflammation, metabolic syndrome and progressive renal disease. Nephrol Dial Transplant. 2009 May;24(5):1384-7. Review. PubMed, PubMedCentral, CrossRef
  11. Golikov PP, Nikolaeva NY. Express – method for determining the activity of angiotensin-converting enzyme in the whey of blood. Clin Lab Diag. 1998; 1: 11-13. (In Russian).
  12. Lushchak VI, Bahniukova TV, Lushchak OV. Indices of oxidative stress. 1. TBA-reactive substances and carbonylproteins. Ukr Biokhim Zhurn. 2004 May-Jun;76(3):136-41. (In Ukrainian). PubMed
  13. Pat. 102192 UA, ICP G01N 33/48 (2006.01).  A Technique for integral evaluate of oxidant-antioxidant balance in blood serum / Korol L. V., Myhal L. Ya. Publ. 10. 06. 2013, Bul.  N 11.  (In Ukrainian).
  14. Farbiszewski R., Worowski K., Rzeczycki W. The application of a modified Sakaguchi’s method for quantitative estimation of protein-bound arginine. Chem Analit.  1972:17(1):133-137.
  15. Pat. 105521 UA, ICP G01N 33/493 (2006.01), A61P 13/12 (2006.01). A Technique for diagnostics of ischemic injury of the tubular nephrothelium in patients with phosphate nephrolithiasis /  Chernenko V. V., Myhal L. Ya., Nikulina H. H., Chernenko D. V., Zheltovska N. I., Klius A. L., Nehrei L. M., Serbina I. Ye., Savchuk V. Yo.  Publ. 25. 03. 2016,  Bul.  N 6.  (In Ukrainian).
  16. Mohkam M, Ghafari A. The Role of Urinary N-acetyl-beta-glucosaminidase in Diagnosis of Kidney Diseases. J Ped Nephrology. 2015;3(3):84-91.
  17. Pechman KR, De Miguel C, Lund H, Leonard EC, Basile DP, Mattson DL. Recovery from renal ischemia-reperfusion injury is associated with altered renal hemodynamics, blunted pressure natriuresis, and sodium-sensitive hypertension. Am J Physiol Regul Integr Comp Physiol. 2009 Nov;297(5):R1358-63.  PubMed, PubMedCentral, CrossRef
  18. Sutariya B, Jhonsa D, Saraf MN. TGF-β: the connecting link between nephropathy and fibrosis. Immunopharmacol Immunotoxicol. 2016;38(1):39-49. Review. PubMed, CrossRef
  19. Heymann F, Trautwein C, Tacke F. Monocytes and macrophages as cellular targets in liver fibrosis. Inflamm Allergy Drug Targets. 2009 Sep;8(4):307-18. Review.  PubMedCrossRef
  20. Tian N, Thrasher KD, Gundy PD, Hughson MD, Manning RD Jr. Antioxidant treatment prevents renal damage and dysfunction and reduces arterial pressure in salt-sensitive hypertension. Hypertension. 2005 May;45(5):934-9.  PubMed, CrossRef

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License.