Ukr.Biochem.J. 2025; Volume 97, Issue 6, Nov-Dec, pp. 134-141

doi: https://doi.org/10.15407/ubj97.06.134

Benzofuran thiazole derivative complexation with polymeric nanoparticles enhances reduction of mitochondrial membrane potential in murine lymphoma cells

16.12.2025   Uncategorized

Ya. R. Shalai1*, A. V. Salamovska1, M. V. Ilkiv1, B. O. Manko1,
Yu. V. Ostapiuk2, N. E. Mitina3, O. S. Zaichenko3, A. M. Babsky1

1Biology Faculty, Ivan Franko National University of Lviv, Ukraine;
2Chemistry Faculty, Ivan Franko National University of Lviv, Ukraine;
3Department of Organic Chemistry, Lviv Polytechnic National University, Ukraine;
*e-mail: Yaryna.Shalay@lnu.edu.ua

Received: 01 August 2025; Revised: 17 September 2025;
Accepted: 28 November 2025; Available on-line:  23 December 2025

The development of new anticancer drugs aimed at the inhibition of mitochondria functioning in tumor cells is a promising approach to cancer treatment. The aim of our study was to investigate the effect of benzofuran derivative N-(5-benzyl-1,3-thiazol-2-yl)-3,5-dimethyl-1-benzofuran-2-carboxamide (BF1) and its complex with polymer nanoparticles based on polyethylene glycol (PEG-PN) on mitochondrial membrane potential in cells of NK/Ly lymphoma grafted in ascite form in mice. Relative values of mitochondrial potential at different exposure times were recorded using the fluorescent dye tetramethylrhodamine. Fluorescence microscopy showed a significant decrease in mitochondrial potential after 30 and 60 min of cells incubation with the BF1-PEG-PN complex but not with unconjugated BF1. After 120 min of incubation, a decrease in the studied parameter was observed under the action of both BF1 alone and its complex with PEG-PN. The data obtained showed that a possible mechanism of cytotoxic action of the BF1 complex with PEG-PN involves early mitochondria depolarization in lymphoma cells.

Keywords: , , , ,

References:

  1. Zong Y, Li H, Liao P, Chen L, Pan Y, Zheng Y, Zhang C, Liu D, Zheng M, Gao J. Mitochondrial dysfunction: mechanisms and advances in therapy. Signal Transduct Target Ther. 2024;9(1):124. PubMed, PubMedCentral, CrossRef
  2. Porporato PE, Filigheddu N, Pedro JMB, Kroemer G, Galluzzi L. Mitochondrial metabolism and cancer. Cell Res. 2018;28(3):265-280. PubMed, PubMedCentral, CrossRef
  3. Rodrigues T, Ferraz LS. Therapeutic potential of targeting mitochondrial dynamics in cancer. Biochem Pharmacol. 2020;182:114282. PubMed, cr id=”https://doi.org/10.1016/j.bcp.2020.114282″]
  4. Hreniukh VP, Finiuk NS, Shalai YaR, Manko BO, Manko BV, OstapiukYuV, Kulachkovskyy OR, Obushak MD, Stoika RS, BabskyAM. Effects of thiazole derivatives on intracellular structure and functions in murine lymphoma cells. Ukr Biochem J. 2020;92(2):121-130. CrossRef
  5. Shalai Ya, Ilkiv M, Salamovska A, Mazur H, Manko B, Ostapiuk Y, Mitina N, Zaichenko O, Babsky A. Changes in bioenergetic characteristics of the murine lymphoma cells under the action of a thiazole derivative in complex with polymeric nanoparticles. Studia Biologica. 2024;18(3):37-46. CrossRef
  6. Ostapiuk YV, Ostapiuk MY, Barabash OV, Kravets M, Herzberger C, Namyslo JC, Obushak MD, Schmidt A. One-pot syntheses of substituted 2 aminothiazoles and 2-aminoselenazoles via Meerwein arylation of alkyl vinyl ketones. Synthesis. 2022;54(16):3658-3666. CrossRef
  7. iniuk N, Klyuchivska O, Ivasechko I, Hreniukh V, Ostapiuk Y, Shalai Y, Panchuk R, Matiychuk V, Obushak M, Stoika R, Babsky A. Proapoptotic effects of novel thiazole derivative on human glioma cells. Anticancer Drugs. 2019;30(1):27-37. PubMed, CrossRef
  8. Mitina NYe, Riabtseva AO, Garamus VM, Lesyk RB, Volyanyuk KA, Izhyk OB, Zaichenko OS. Morphology of the micelles formed by a comb-like PEG-containing copolymer loaded with antitumor substances with different water solubilities. Ukr J Phys. 2020;65(8):670. CrossRef
  9. Ilkiv MV, Shalai YaR, Mazur HM, Manko BO, Manko BV, Ostapiuk YuV, Mitina NE., Zaichenko AS, Babsky AM. Bioenergetic characteristics of the murine Nemeth-Kellner lymphoma cells exposed to thiazole derivative in complex with polymeric nanoparticles. Ukr Biochem J. 2022;94(6):30-36. CrossRef
  10. Schober P, Boer C, Schwarte LA. Correlation Coefficients: Appropriate Use and Interpretation. Anesth Analg. 2018;126(5):1763-1768. PubMed, CrossRef
  11. Trotta AP, Gelles JD, Serasinghe MN, Loi P, Arbiser JL, Chipuk JE. Disruption of mitochondrial electron transport chain function potentiates the pro-apoptotic effects of MAPK inhibition. J Biol Chem. 2017;292(28):11727-11739. PubMed, PubMedCentral, CrossRef
  12. Pokrzywinski KL, Biel TG, Kryndushkin D, Rao VA. Therapeutic Targeting of the Mitochondria Initiates Excessive Superoxide Production and Mitochondrial Depolarization Causing Decreased mtDNA Integrity. PLoS One. 2016;11(12):e0168283. PubMed, PubMedCentral, CrossRef
  13. Zhang W, Hu X, Shen Q, Xing D. Mitochondria-specific drug release and reactive oxygen species burst induced by polyprodrug nanoreactors can enhance chemotherapy. Nat Commun. 2019;10(1):1704. PubMed, PubMedCentral, CrossRef
  14. lkiv MV, Shalai YaR, Manko BO, Ostapiuk YuV, Mitina NE, Zaichenko AS, Babsky AM. Generation of ROS under the influence of thiazole derivative and its complexes with PEG-based polymeric nanoparticles. Biopolym Cell. 2022;38(3):158-168. CrossRef
  15. Środa-Pomianek K, Michalak K, Świątek P, Poła A, Palko-Łabuz A, Wesołowska O. Increased lipid peroxidation, apoptosis and selective cytotoxicity in colon cancer cell line LoVo and its doxorubicin-resistant subline LoVo/Dx in the presence of newly synthesized phenothiazine derivatives. Biomed Pharmacother. 2018;106:624-636. PubMed, CrossRef
  16. Gurunathan S, Jeyaraj M, Kang MH, Kim JH. Tangeretin-Assisted Platinum Nanoparticles Enhance the Apoptotic Properties of Doxorubicin: Combination Therapy for Osteosarcoma Treatment. Nanomaterials (Basel). 2019;9(8):1089. PubMed, PubMedCentral, CrossRef
  17. Yuan YG, Peng QL, Gurunathan S. Combination of palladium nanoparticles and tubastatin-A potentiates apoptosis in human breast cancer cells: a novel therapeutic approach for cancer. Int J Nanomedicine. 2017;12:6503-6520.  PubMed, PubMedCentral, CrossRef
  18. Zeng X, Morgenstern R, Nyström AM. Nanoparticle-directed sub-cellular localization of doxorubicin and the sensitization breast cancer cells by circumventing GST-mediated drug resistance. Biomaterials. 2014;35(4):1227-1239. PubMed, CrossRef
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