Tag Archives: complexation
Benzofuran thiazole derivative complexation with polymeric nanoparticles enhances reduction of mitochondrial membrane potential in murine lymphoma cells
Ya. R. Shalai1*, A. V. Salamovska1, M. V. Ilkiv1, B. O. Manko1,
Yu. V. Ostapiuk2, N. E. Mitina3, O. S. Zaichenko3, A. M. Babsky1
1Biology Faculty, Ivan Franko National University of Lviv, Ukraine;
2Chemistry Faculty, Ivan Franko National University of Lviv, Ukraine;
3Department of Organic Chemistry, Lviv Polytechnic National University, Ukraine;
*e-mail: Yaryna.Shalay@lnu.edu.ua
Received: 01 August 2025; Revised: 17 September 2025;
Accepted: 28 November 2025; Available on-line: 2025
The development of new anticancer drugs aimed at the inhibition of mitochondria functioning in tumor cells is a promising approach to cancer treatment. The aim of our study was to investigate the effect of benzofuran derivative N-(5-benzyl-1,3-thiazol-2-yl)-3,5-dimethyl-1-benzofuran-2-carboxamide (BF1) and its complex with polymer nanoparticles based on polyethylene glycol (PEG-PN) on mitochondrial membrane potential in cells of NK/Ly lymphoma grafted in ascite form in mice. Relative values of mitochondrial potential at different exposure times were recorded using the fluorescent dye tetramethylrhodamine. Fluorescence microscopy showed a significant decrease in mitochondrial potential after 30 and 60 min of cells incubation with the BF1-PEG-PN complex but not with unconjugated BF1. After 120 min of incubation, a decrease in the studied parameter was observed under the action of both BF1 alone and its complex with PEG-PN. The data obtained showed that a possible mechanism of cytotoxic action of the BF1 complex with PEG-PN involves early mitochondria depolarization in lymphoma cells.
Nature of the interaction of Са(2+) with D-glucosamine: DFT quantum chemical study
M. M. Ilchenko
Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv;
e-mail: m.m.ilchenko@imbg.org.ua
Geometric and electronic structure of D-glucosamine complexes with Ca2+ was studied with DFT (Density Functional Theory) method at B3LYP/6-31G(d) level of the theory. It was shown that in the most probable complex the cation interacts directly with oxygen atom of glucopyranose ring. The presence of six water molecules in the first hydration sphere of Ca2+ does not substantially affect the order of energetic favorability of complexes formed by different modes of cation coordination. Calculations within the frame of PCM model demonstrated that in aqueous solution the only stable complexes are those where Ca2+ interacts directly with glucopyranose ring oxygen. This interaction between the cation and glucosamine may be accompanied by deprotonation of О1Н hydroxyl group and simultaneous protonation of amino group.