Tag Archives: oxidative stress
Indices of antioxidant and osmoprotective systems in seedlings of winter wheat cultivars with different frost resistance
T. O. Yastreb1, Yu. E. Kolupaev1,2*, A. I. Kokorev1, B. E. Маkaova2,
N. I. Ryabchun1, O. A. Zmiievska1, G. D. Pospielova2
1Yuriev Plant Production Institute, National Academy of Agrarian Sciences of Ukraine, Kharkiv;
2Poltava State Agrarian University, Ukraine;
*e-mail: plant_biology@ukr.net
Received: 02 January 2023; Revised: 27 January 2023;
Accepted: 13 April 2023; Available on-line: 27 April 2023
The functioning of the stress-protective systems of wheat under the action of cold at the early stages of plant development remains poorly studied. The aim of this work was a comparative study of antioxidant activity and the content of sugars and proline as indicators of osmoprotective activity during cold adaptation of seedlings of seven winter bread wheat (Triticum aestivum L.) cultivars that differ significantly in frost resistance. The 3-day-old etiolated seedlings were hardened at 2°C for 6 days and then frozen for 5 h at -6 or -9°C. Two days after freezing, the survival of seedlings was assessed by their ability to grow. A decrease in ROS content, an increase in the activity of antioxidant enzymes catalase and guaiacol peroxidase and accumulation of sugars in the shoots of high-frost-resistant cultivars during hardening were detected. The absolute values of catalase and guaiacol peroxidase activity correlated positively with the frost resistance of seedlings. The negative correlation between the frost tolerance of the cultivars and the accumulation of proline in the seedlings during hardening was recorded. The possibility of using the studied biochemical indices for frost resistance screening of winter wheat varieties at the seedling stage was stated.
Rhabdomyolysis attenuates activity of semicarbazide sensitive amine oxidase as the marker of nephropathy in diabetic rats
O. Hudkova*, I. Krysiuk, L. Drobot, N. Latyshko
Department of Cell Signaling, Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
*e-mail: ogudkova@biohem.kiev.ua
Received: 22 December 2021; Accepted: 21 January 2022
Amine oxidases are involved in the progression of many diseases and their complications, including renal failure, due to the generation of the three toxic metabolites (H2O2, aldehydes, and ammonia) in the course of biogenic amines oxidative deamination. The participation of the first two products in kidney pathogenesis was confirmed, whereas the role of ammonia as a potential inducer of the nitrozative stress is not yet understood. The aim of the present study was to test how further intensification of oxidative stress would affect diabetes-mediated metabolic changes. For this purpose, a rat model of glycerol-induced rhabdomyolysis, as a source of powerful oxidative stress due to the release of labile Fe3+ from ruptured myocytes, on the background of streptozotocin-induced diabetes was used. The experimental animal groups were as follows: group 1 – ‘Control’, group 2 – ‘Diabetes’, group 3 – ‘Diabetes + rhabdomyolysis’. A multifold increase in semicarbazide sensitive amine oxidase (SSAO) activity in the kidney and blood, free radicals (FR), MetHb and 3-nitrotyrosine (3-NT) levels in the blood, as well as the emergence of HbNO in plasma and dinitrosyl iron complexes (DNICs) in the liver of animals in group 2 as compared to control were revealed. An additional increase in FR, HbNO levels in the blood, and DNICs in the liver of animals in the diabetes + rhabdomyolysis group as compared to the diabetes group, which correlated with the appearance of a large amount of Fe3+ in the blood of group 3 animals, was detected. Unexpectedly, we observed the positive regulatory effects in animals of the diabetes + rhabdomyolysis group, in particular, a decreased SSAO activity in the kidney and 3-NT level in plasma, as well as the normalization of activity of pro- and antioxidant enzymes in the blood and liver compared to animals of diabetes group. These consequences mediated by rhabdomyolysis may be the result of NO exclusion from the circulation due to the excessive formation of NO stable complexes in the blood and liver. The data obtained allow us to consider SSAO activity as a marker of renal failure in diabetes mellitus. In addition, we suggest a significant role of nitrosative stress in the development of pathology, and, therefore, recommend NO-traps in the complex treatment of diabetic complications.
Oxidative stress in rat heart mitochondria under a rotenone model of Parkinson’ disease: a corrective effect of capicor treatment
O. O. Gonchar*, O. O. Klymenko, T. I. Drevytska,
L. V. Bratus, I. M. Mankovska
Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv;
*e-mail: olga.gonchar@i.ua
Received: 22 March 2021; Accepted: 22 September 2021
Biochemical and genetic mechanisms of oxidative stress (OS) developing in rat heart mitochondria were studied in a rotenone model of Parkinson’s disease (PD), and the effect of Capicor (combination of meldonium dihydrate and gamma-butyrobetain dihydrate) on these mechanisms was evaluated. Experiments were carried out on adult male Wistar rats: I – intact rats (control); II –with rotenone administration subcutaneously at dose 3 mg/kg per day along 2 weeks; III – with rotenone/Capicor administration: after rotenone intoxication, capicor was injected intraperitoneally at dose 50 mg/kg per day along following 2 weeks. As OS biomarkers, lipid peroxidation, protein oxidative modification, H2O2 production, the activity of MnSOD, GPx and glutathione pool indexes were measured. The PD-related genes Parkin (PARK2) and DJ-1 (PARK7) as well as MnSOD and DJ-1 protein expressions were detected. Rotenone intoxication increased the intensity of lipid peroxidation, protein oxidative modification, and H2O2 production. These events were accompanied by decreased in GSH content, GSH/GSSG ratio, and GPx activity. Increased ROS production and impaired antioxidant defenses could result from the established DJ-1 gene and DJ-1 protein deficiency. Capicor administration increased the endogenous antioxidant defense, weakening the lipid peroxidation and oxidative modification of mitochondrial proteins. Capicor treatment led to an increase in GSH content and GSH/GSSG ratio in heart mitochondria that may serve as additional indicators of the OS intensity reducing. Capicor promoted overexpression of DJ-1 and PARK2 genes in the heart that may indicate a rise in mitophagy and a decrease in OS.
Protective effect of Atriplex halimus extract against benzene-induced haematotoxicity in rats
K. Zeghib1*, D. A. Boutlelis2, S. Menai3, M. Debouba4
1Department of chemistry, Faculty of exact sciences, University of El-Oued, El-Oued, Algeria;
2Department of Biology, Faculty of natural sciences and life, University of El-Oued, El-Oued, Algeria;
3The mother-child hospital (Bachir Bennacer) of El-Oued, El-Oued, Algeria;
4Higher Institute of Applied Biology of Medenine, University of Gabès, Tunisia;
*e-mail: zeghib-khaoula@univ-eloued.dz
Received: 24 December 2020; Accepted: 07 July 2021
Benzen (BZ) is a ubiquitous environmental pollutant with a toxic effect mainly aimed at the hematopoietic and immune systems. Atriplex halimus L. (Amaranthaceae) is a Mediterranean halophytic shrub traditionally used in North Africa as medicinal plant for several therapeutic uses. The present study aimed to estimate the preventive and curative effects of Atriplex halimus L. (Ah) aqueous extract against BZ-induced hematotoxicity in rats. Analysis of the extract by the method of LC-MS revealed the presence of 7 vitamins, among which vitamin C content was the highest. Adult rats were divided into five groups as follow: Group 1 received water (control); Group 2 received orally Ah aqueous extract (200 mg/kg) 3 days/week for 15 weeks; Group 3 received BZ (100 mg/kg b.w) daily in drinking water for 15 weeks; Group 4 received concomitantly BZ (100 mg/kg) and preventive treatment with Ah (200 mg/kg) for 15 weeks (AhP+BZ); Group 5 first received BZ (100 mg/kg) for 11 weeks and then curative treatment with Ah extract (300 mg/kg) daily for 30 days (BZ+AhC). It was shown that sub-chronic exposure to benzene induced leukopenia, lymphocytopenia, granulocytopenia and massive degeneration of the bone marrow tissue. The level of GSH and activity of GST and CAT were significantly lowered and the level of MDA was increased in the blood and bone marrow in rats of BZ-intoxicated group compared to the control rats. Administration of Ah extract recovered the bone marrow structure, dramatically decreased MDA content and increased GSH and CAT activity and GST level in the blood and bone marrow as compared with the indices in BZ-treated group. These observations demonstrate that curative and, to a lesser extent, preventive treatment with Atriplex halimus extract have therapeutic potential against hematotoxicity induced by benzene.
Sex dependent differences in oxidative stress in the heart of rats with type 2 diabetes
N. I. Gorbenko1*, O. Yu. Borikov2, O. V. Ivanova1, T. V. Kiprych1,
E. V. Taran1, T. I. Gopciy2, Т. S. Litvinova1
1SI “V. Danilevsky Institute for Endocrine Pathology Problems of the NAMS of Ukraine”, Kharkiv;
2V.N. Karazin Kharkiv National University, Kharkov, Ukraine;.
*е-mail: Gorbenkonat58@ukr.net
Received: 17 September 2020; Accepted: 17 May 2021
Type 2 diabetes mellitus is known to double mortality from cardiovascular diseases (CVD), in which oxidative stress plays an important role. It is suggested that the impact of diabetes on CVD risk may vary depending on gender. The aim of the study was to assess oxidative stress parameters in the heart of 12 weeks old male and female Wistar rats with type 2 diabetes mellitus (T2DM) induced by high-calorie diet followed by intraperitoneal streptozotocin injections. The level of advanced oxidation protein products, superoxide dismutase, glutathione reductase and glutathione peroxidase activity in the isolated heart mitochondria and NADPH-oxidase and xanthine oxidase activity in the post-mitochondrial supernatant fraction were determined. It was shown that T2DM induced more pronounced oxidative stress confirmed by the increased level of advanced oxidation protein products in the heart mitochondria of males than females. The data obtained indicate that the main reason of oxidative stress in the heart of diabetic males is the activation of non-mitochondrial sources of reactive oxygen species. While in the heart of diabetic female rats it is the decrease in antioxidant enzymes activity in mitochondria. These results justify the necessity of gender-specific therapy for the prevention and management of diabetic CVD.
Oxidative stress suppression contributes to antiseizure action of axitinib and rapamycin in pentylenetetrazol-induced kindling
O. B. Poshyvak1*, O. R. Pinyazhko1,2, L. S. Godlevsky3
1Pharmacology Department, Danylo Halytsky Lviv National Medical University, Lviv, Ukraine;
2Department of Civilization Diseases and Regenerative Medicine, WSIiZ, Rzeszow, Poland;
3Department of Biophysics, Informatics and Medical Devices, Odesa National Medical University, Odesa, Ukraine;
*e-mail: olesya.poshyvak@gmail.com
Received: 29 January 2021; Accepted: 23 April 2021
Rapamycin and axitinib block different kinases in signaling pathways such as PI3K-Akt-mTOR and BDNF-TrkB, respectively. Both have antiseizure and antioxidative actions, which justify studying the combined effects of these drugs upon seizures and oxidative stress in the chronic model of epilepsy. The investigation aimed to look for the combined effect of rapamycin and axitinib upon pentylenetetrazol (PTZ)-kindled seizures and oxidative stress. Experiments were performed on 300 two- to four-month-old Wistar male rats, which had been kindled daily with PTZ (35.0 mg/kg, i.p.). Malondialdehyde (MDA) level, superoxide dismutase (SOD) activity, and glutathione (GSH) level were determined in brain tissues of kindled rats before and after the treatment. The analysis of antiseizure and antioxidative actions was performed using ED50 of rapamycin and axitinib, with their combined administration using graded dosages of ED50 of each drug. The median effective dose (ED50) for rapamycin and axitinib was 0.93 and 4.97 mg/kg, respectively. ED50 of rapamycin when combined with axitinib (2.0 mg/kg) was 0.60 mg/kg, which was reduced by 35.6% when compared with the ED50 administered alone (P < 0.05). The MDA level increased from 152.9±24.8 to 388.3±49.2 nmol/mg of protein (P < 0.05), while SOD activity reduced from 11.14±2.33 to 3.54±1.08 IU/mg of protein (P < 0.05) in brain tissues of the kindled rats. Combined treatment with rapamycin (0.56 mg/kg, i.p.) and axitinib (2.0 mg/kg, i.p.) resulted in a significant rise in SOD activity (11.09±1.86 IU/mg) and GSH level (7.32±1.34 µg/mg) when compared with the kindled rats (P < 0.05). Combined axitinib and rapamycin therapy have an antiepileptic and antioxidative effect on PTZ-kindled seizures.
Effect of N-acetyl cysteine on oxidative stress and Bax and Bcl2 expression in the kidney tissue of rats exposed to lead
M. Gholami1, A. B. Harchegani2, S. Saeedian3,
M. Owrang4, M. R. Parvizi1*
1Department of Physiology, Faculty of Medicine, AJA University of Medical Sciences, Tehran, Iran;
2Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran;
3Medical Genomic Research Center, Tehran Medicine Sciences Islamic Azad University, Tehran, Iran;
4Faculty of Medicine, Sari Branch, Islamic Azad University, Sari, Iran;
*e-mail: mparvizi@alumnus.tums.ac.ir
Received: 30 May 2020; Accepted: 17 December 2021
This study aimed to consider the lead-induced oxidative damage of the kidney of male rats and the role of antioxidant N-acetylcysteine (NAC) in preserving cells against Pb toxicity. Rats were randomly divided into five groups including G1 (control), G2 (single 70 mg/kg dose of Pb), G3 (continuous daily 2 mg/kg dosing of Pb for 4 weeks), G4 (single dose of Pb + 50 mg/kg NAC), and G5 (continuous daily dosing of Pb + 50 mg/kg NAC). The level of malonic dialdehyde (MDA) and total antioxidant capacity were measured spectrophotometrically.The level of Pb in serum and kidney tissue was measured by atomic absorption spectroscopy. Expression of Bax and Bcl2 genes was estimated using RT-PCR. It was shown that single and continuous exposure to Pb caused a considerable increase of Pb content in serum and kidney tissue of rats in G2 and G3 groups compared to other groups. NAC treatment significantly improved TAC values and decreased MDA values in the serum of rats exposed to Pb. Single and continuous Pb dosing caused a 3.9- and 13.1-fold increase in Bax expression and 1.5-fold and 2.1-fold decrease in Bcl2 expression in a kidney tissue respectively. The current study revealed that single and especially continuous Pb exposure was strongly associated with Pb accumulation, antioxidant depletion, oxidative stress and kidney cells apoptosis. NAC can help protect kidney tissue against Pb by elevating antioxidant capacity, mitigating oxidative stress and normalizing Bax and Bcl2 genes expression.