Tag Archives: biochemical parameters
C(60) fullerene attenuates the signs of acute renal failure in rats under rhabdomyolysis due to inhibition of oxidative stress
O. Omelchuk1, S. Prylutska2, D. Nozdrenko3,
O. Motuziuk1,3, I. Vareniuk3, K. Bogutska3,
O. Vygovska4, А. Zholos3, Yu. Prylutskyy3*
1Lesya Ukrainka Volyn National University, Lutsk, Ukraine;
2National University of Life and Environmental Science of Ukraine, Kyiv, Ukraine;
3Taras Shevchenko National University of Kyiv, Ukraine;
*e-mail: prylut@ukr.net;
4Bogomolets National Medical University, Kyiv, Ukraine
Received: 14 August 2023; Revised: 18 September 2023;
Accepted: 27 October 2023; Available on-line: 06 November 2023
Rhabdomyolysis, as an acute stage of myopathy is known to be associated with the accumulation of muscle breakdown products, acute renal failure and oxidative stress. The goal of the study was to evaluate the effect of C60 fullerene as an antioxidant on kidney damage in the model of glycerol-induced rhabdomyolysis in rats. The study was conducted on male Wistar rats, divided into the following experimental groups: control animals, animals intramuscularly injected with glycerol in a doses of 5, 10 and 15 mg/kg and those intraperitoneally injected daily with C60 fullerene aqueous solution (C60FAS) in a dose of 1 or 2 mg/kg at 48 h after glycerol administration. Monitoring of the biochemical and morphological indicators was carried out on 3rd, 6th and 9th days of the experiment. A close correlation between the acute renal damage severity, increased creatinine and urea level, superoxide dismutase (SOD) and catalase (CAT) activity in the blood of rats was observed. It was shown that in rats which received 2 mg/kg of C60FAS the renal glomeruli size and necrosis manifestations were attenuated, whereas SOD and CAT activity in the blood was significantly decreased. The results obtained may be useful for developing approaches to the treatment of pathological conditions of the muscular system caused by rhabdomyolysis and associated oxidative stress.
Indoleamine 2,3-dioxygenase level and oxidative stress parameters in the serum of patients with chronic renal failure
F. M. Y. Saeed, R. F. Jasim*
College of Education for Girls, Department of Chemistry, University of Mosul, Iraq;
*e-mail: ra.fadhel@uomosul.edu.iq
Received: 13 April 2023; Revised: 02 June 2023;
Accepted: 07 September 2023; Available on-line: 12 September 2023
Indoleamine 2,3-dioxygenase (IDO) is a tryptophan-degrading enzyme belonging to the kynurenine pathway. IDO activity has been suggested as a biomarker for diagnosis of chronic kidney disease. The aim of the study was to estimate the level of IDO, urea, creatinine, uric acid, phosphate, calcium, albumin, MDA, GSH, and activity of peroxidase, catalase, arylesterase in the serum of chronic renal failure (CRF) patients treated with dialysis compared to the healthy control group. The results showed a significant increment in IDO level in patients compared with the control. Linear regression analysis using the Pearson correlation coefficient showed that increased IDO level correlates positively with urea, creatinine, uric acid, phosphate, MDA level and peroxidase activity whereas negatively with albumin, calcium, glutathione level, catalase activity and glomerular filtration rate. We concluded that IDO level might be a possible marker of oxidative stress and inflammation in patients with CRF.