Tag Archives: chronic wounds
Acellular dermal matrix for chronic wound treatment: dry or wet?
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1Department of Histology and Embryology, Ivan Horbachevsky Ternopil National Medical University, Ternopil, Ukraine;
2Department of Medical Biochemistry, Ivan Horbachevsky Ternopil
National Medical University, Ternopil, Ukraine;
3Department of Pathophysiology, Ivan Horbachevsky Ternopil
National Medical University, Ternopil, Ukraine;
4Central Research Laboratory, Ivan Horbachevsky Ternopil
National Medical University, Ternopil, Ukraine;
5Department of Pharmacology and Clinical Pharmacology,
Ivan Horbachevsky Ternopil National Medical University, Ternopil, Ukraine;
*e-mail: shevchukoo@tdmu.edu.ua
Received: 27 May 2026; Revised: 19 June 2026;
Accepted: 27 July 2026; Available on-line: 04 August 2026
Background. Chronic wounds pose a serious clinical and economic burden, and their effective treatment requires the development of new biomaterials that promote tissue regeneration. Acellular dermal matrices (ADMs) represent a promising regenerative strategy; however, their biological properties largely depend on the manufacturing protocol. Objective. To compare the biocompatibility and cytotoxicity of two porcine acellular dermal matrix variants, dry (d-ADM) and wet (w-ADM), and to evaluate the therapeutic efficacy of the biocompatible matrix in an experimental porcine model of chronic full-thickness wounds. Methods. Biocompatibility was assessed in vitro using human umbilical cord-derived mesenchymal stem cells (hU-MSCs) by evaluating cell morphology, viability, proliferation, and lactate dehydrogenase (LDH) release. The therapeutic efficacy of the selected ADM was investigated in a porcine chronic full-thickness excisional wound model and compared with non-adhesive dressings and Promogran Prisma. Wound healing was evaluated after 28 days using histological, immunohistochemical (TNF-α, TGF-β, Ki-67), and morphometric analyses. Results. The wet ADM preserved hU-MSC morphology, viability, and proliferative capacity, whereas the dry ADM induced marked cytotoxicity, increased LDH release, and extensive cell death. In vivo, treatment with w-ADM significantly accelerated wound healing compared with both control dressings, resulting in complete and stable re-epithelialization, minimal inflammatory infiltration, organized collagen remodeling, and improved tissue architecture. Immunohistochemical analysis demonstrated significantly lower expression of TNF-α, TGF-β, and Ki-67, indicating resolution of inflammation and transition toward tissue remodeling and regenerative stabilization. Conclusions. Wet acellular dermal matrix demonstrated excellent biocompatibility and superior regenerative performance, whereas the dry matrix exhibited unacceptable cytotoxicity. Wet ADM represents a promising scaffold for regenerative treatment of chronic wounds and warrants further investigation as a clinically applicable biomaterial, including in combination with mesenchymal stem cell-based therapies.
Levels of angiogenic regulators and MMP-2, -9 activities in Martorell ulcer: a case report
O. M. Petrenko1, A. A. Tykhomyrov2
1Bogomolets National Medical University, Kyiv, Ukraine;
2Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv, Ukraine;
e-mail: artem_tykhomyrov@ukr.net
Received: 27 July 2018; Accepted: 13 December 2018
Martorell hypertensive ischemic leg ulcers (HYTILU) represent a unique form of lower extremity non-healing ulcers that develop in association with poorly controlled high blood pressure. The present study was performed in order to assess levels of protein regulators of angiogenesis (vascular endothelial growth factor, or VEGF, and angiostatins) and to evaluate activities of matrix metalloproteinases (MMPs) (gelatinases MMP-2 and -9) in wound cutaneous tissue in the case of patient with 2-years HYTILU history. VEGF and angiostatin levels were analyzed by Western blot, MMP activities were evaluated by gelatin zymography. We report here for the first time that wound tissue in HYTILU is characterized with increased levels of VEGF (by 75 folds vs. histologically normal tissue, P < 0.01) and dramatic overproduction of angiostatin levels, which are undetectable in healthy cutaneous tissue. Approximately 10-fold elevation in MMP-2 and -9 activities is observed in wound tissue as compared with uninjured cutaneous tissue. Obtained results indicate that increased production of angiogenic inhibitors, angiostatins, may counteract VEGF-induced pro-angiogenic signaling, and together with MMP overactivation, contributes to failed healing of ischemic ulcer. Further extended studies are needed to clarify how changes of angiogenic profile and imbalance of proteolytic activities in non-healing Martorell ulcers can be considered during their management procedures to improve efficacy of surgery debridement and/or skin grafting.