S. M. Grebinyk, I. I. Grynyuk, S. V. Prylutska, O. P. Matyshevska

Taras Shevchenko Kyiv National University, Ukraine;
e-mail: grebnik_z@yahoo.com

The dynamics of active oxygen forms (AOF) generation in rat thymocytes 50 min after treatment with 0.1 and 0.5 mM H₂O₂ was estimated with the use of fluorescent probe DCFDA. Both enhanced AOF generation, which was dependent on H₂O₂ concentration, and glutathione peroxidase and superoxide dismutase activation, followed by a decrease of thymocytes viability were demonstrated.
Preincubation of cells with 10^-5 M fullerene C₆₀ was shown not only to prevent H₂O₂ – induced AOF generation but to increase viability of H₂O₂-treated thymocytes at more prolonged time period. The data obtained indicate to fullerene C₆₀ ability to prevent oxidative stress in thymocytes.