Tag Archives: fungicides
Fatty acids composition of kidneys under rat exposure to fungicides
S. V. Khyzhnyak*, S. V. Midyk, О. В. Dovbysh, V. I. Korniyenko
National University of Life and Environmental Sciences of Ukraine, Kyiv;
*e-mail: khyzhnyaks@gmail.com
Received: 08 August 2024; Revised: 10 December 2024;
Accepted: 21 February 2025; Available on-line: 03 March 2025
Most fungicides belong to the class of triazoles, of which tebuconazole and triadimefon are the most commonly used. Exposure to pesticides is accompanied by negative consequences for vertebrates and humans. However, the potential toxicity of fungicides is not well understood. The aim of the study was to estimate the fatty acids (FAs) composition of kidney total lipids after exposure of Wistar han rats to two fungicide preparations, one containing tebuconazole alone, the other tebuconazole + triadimephone. Animals were administered a single oral dose of fungicides (600 and 1200 mg/kg). After 14 days, the animals were decapitated and kidneys were used in the studies. FAs methyl esters were determined on a Trace GC Ultra gas chromatograph (USA) with the flame ionization detector. No qualitative changes in the FAs profile of the total kidney lipids after fungicides administration as compared to the control were detected, but the redistribution in the content of individual FAs was observed. The relative content of saturated fatty acids increased, while that of monounsaturated fatty acids, in particular oleic acid (C18:1ω9), decreased. An increase in the content of long-chain polyunsaturated fatty acids, in particular docosahexaenoic (C22:6ω3) and arachidonic (C20:4ω6) acids, as well as the value of the ratio ω3/ω6, was revealed. The effect of the combined fungicides was found to be more pronounced. The results indicate metabolic shift in the FAs profile of total kidney lipids in the dynamics after exposure to fungicides.
Fatty acid profile of the liver lipids under acute fungicide action and intake of a biologically active preparation in rats
S. V. Khyzhnyak*, S. V. Midyk, A. O. Velinska,
О. V. Arnauta, L. Н. Kalachniuk
National University of Life and Environmental Sciences of Ukraine, Kyiv;
*e-mail: khyzhnyaks@gmail.com
Received: 18 August 2022; Revised: 24 October 2022;
Accepted: 04 November 2022; Available on-line: 14 November 2022
The widespread use of fungicides in agriculture leads to a negative consequences for both humans and the environment. The acute effect of fungicides containing tebuconazole alone or in combination with triadimefon on fatty acid (FAs) composition of the total lipids in the liver of rats was studied. FAs content was analyzed by gas chromatography with the use of flame ionization detector. The results obtained indicate that the acute effect of triazole fungicides one-time oral administration to rats is characterized by toxic reactions and is accompanied by the total liver lipids FAs profile redistribution, similar for the studied fungicides. In particular, the reduction in the total content of monounsaturated FAs (by 26% – 37%) and in the ω3/ω6 ratio was observed. It was demonstrated that the subsequent two-week introduction of the preparation based on Milk thistle seeds oil extract had a positive effect and improved the FAs profile of lipids in dynamics after fungicide intake.