Tag Archives: interferon

Studying the interaction of 2′-5′-oligoadenylate and their analogues with proteins by fluorescence spectroscopy

Z. Yu. Tkachuk1, L. V. Dubey1, V. V. Tkachuk1, L. V. Tkachuk1,
M. Yu. Losytskyy2, V. M. Yashchuk2, І. Ya. Dubey1

1Institute of Molecular Biology and Genetics,
National Academy of Sciences of Ukraine, Kyiv;
2Taras Shevchenko Kyiv National University,Ukraine;
e-mail: ztkachuk@bigmir.net

The interaction of “core” 2′-5′-oligoadenylates (2-5A) and their analogues with proteins albumin, interferon and immunoglobulin G was studied by fluorescence spectroscopy methods. Strong quenching of protein fluorescence (up to 67%) was observed upon interaction of oligoadenylates in concentration of 5×10-5 М with albumin. Investigated compounds quenched the emission of interferon to a lesser extent, whereas no significant fluo­rescence changes occurred upon interaction with immunoglobulin under the same conditions. The level of quenching depended on the structure of 2-5A compounds and decreased with the decrease of their concentration. These data suggest that 2-5A actively bind to albumin and less efficiently to interferon, and practically do not interact with immunoglobulin. Taking into consideration different efficiency of quenching of the fluorescence excited at 280 and 296 nm, the assumption has been made about a possible role of tyrosine and tryptophan in the binding of a given compound to proteins. Possible mechanisms of interaction of oligoadenylates with proteins are discussed.

Laureates of the Palladin Prize of the National Academy of Sciences of Ukraine (2008, 2011)

R. P. Vynogradova, V. M. Danilova

Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
e-mail: valdan@biochem.kiev.ua

In 2008 Z. R. Ulberg, O. V. Karpov and S. V. Veryovka were awarded the Palladin Prize of NAS of Ukraine for the series of scientific works “Colloid-chemical and physical-biochemical aspects of the interaction of nano- and microparticles with cells as a basis for the development of nanobiotechnology ” and in 2011 M. Ya. Spivak, L. M. Lazarenko and N. M. Zholobak received the Prize for the series of works “Molecular and biological characteristics of interferon and creation of scientific approaches to the application of interferon medications and their inducers under pathology“. The article also provides laureates’ biographies.