Tag Archives: mesenchymal stromal cells
The effect of thymic mesenchymal stromal cells on arginase activity and nitric oxide produced by mouse macrophages
R. S. Dovgiy1,2, I. S. Nikolsky3, L. M. Skivka1
1Taras Shevchenko National University of Kyiv, Ukraine;
2Institute of Gerontology, NAMS of Ukraine, Kyiv;
3State Institute of Genetic and Regenerative
Medicine NAMS of Ukraine, Kyiv;
e-mail: romandovgiy@gmail.com
Mesenchymal stromal cells (MSC) gained much attention due to their therapeutic properties, mediated largely by anti-inflammatory action. We aimed to investigate the capacity of MSC obtained from young mice to modulate arginine metabolism of macrophages from old animals. Bone marrow cells obtained from young and aged mice were cocultivated with MSC in the presence of M-CSF. Nitric oxide production was analyzed in supernatants by Griess reaction, and arginase activity was measured in cell lysates. We have found that arginase activity was significantly lower in macrophages isolated from old mice as compared to young animals (P ˂ 0.05). Syngeneic MSC addition markedly stimulated arginase activity in macrophages from both young and aged mice (P ˂ 0.001), with greater effect in old animals. There were no significant differences in nitric oxide level between groups. In summary, there was more pronounced anti-inflammatory shift in macrophage metabolism in aged animals upon cocultivation with MSC.
The influence of low-molecular fraction from cord blood (below 5 kDa) on functional and biochemical parameters of cells in vitro
A. K. Gulevsky, N. N. Moisieieva, O. L. Gorina,
J. S. Akhatova, A. A. Lavrik, A. V. Trifonova
Institute for Problems of Cryobiology and Cryomedicine,
National Academy of Sciences of Ukraine, Kharkiv;
e-mail: moiseeva-nataly@rambler.ru
The influence of a low-molecular fraction (below 5 kDa) from the cattle cord blood (CBF) on functional activity of phagocytes, human embryonic fibroblasts, mesenchymal stromal cells and BHK-21 clone 13/04 and PK-15 cells was studied. The low-molecular fraction added to culture medium increases the growth rate of cell cultures. The incubation of leukoconcentrate in the CBF-containing medium results in an increase in phagocytic indices of neutrophils in the presence of a phagocytosis inhibitor – sodium iodoacetate, leading to a significant increase in intracellular glucose content and alkaline phosphatase activity as compared to the control and the reference drug Actovegin®.