Tag Archives: metallothionein

Evaluation of biological effects of cobalt-nanocomposites with the use of biochemical markers of bivalve mollusk Anodonta cygnea

H. I. Falfushynska1, L. L. Gnatyshyna1, O. B. Stoliar1, N. E. Mitina2, O. S. Zaichenko2, Ye. Z. Filyak3, R. S. Stoika3

1Volodymyr Hnatiuk Ternopil National Pedagogical University, Ukraine;
e-mail: halynka.f@gmail.com,  oksana.stolyar@gmail.com;
2Lviv Polytechnic National University, Ukraine;
3Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv;
e-mail: stoika@cellbiol.lviv.ua

Intensive implementation of nanomaterials requires development of novel methods for evalua­tion of their potential ecotoxicity. The aim of our study was to identify specific characteristics of the effect of cobalt-nanocomposite (Co-NC) on the molecular stress-responsive system in the digestive gland of bivalve mollusk Anodonta cygnea. Nanocomposite was synthesized by mixing alcohol solution of copolymer N-vinylpirrolidone, 5-(tret-butylperoxy)-5-methyl-1-hexene-3-yne and dimethylaminoethylmetacrylate and cobalt (II) chloride. After 14 days of the mollusk exposure in the presence of Co-NC, CoCl2 or corresponding polymer substance it was shown that the Co NC, in contrast to other agents, does not cause an oxidative stress due to the superoxide dismutase activity, metallotioneins (MTs) level, glutathione redox index and oxyradical production. Multivaria­te analysis confirmed specific features of the Co NC’s effect related to an enhanced expression of MTs, while CoCl2 activated lactate dehydrogenate and oxyradical production, and polymer substance enhanced glutathione transferase activity.

Corvitin restores metallothionein and glial fibrillary acidic protein levels in rat brain affected by pituitrin-izadrin

H. N. Shiyntum1, O. O. Dovban1, Y. P. Kovalchuk1,
T. Ya. Yaroshenko2, G. A. Ushakova1

1Oles Honchar Dnipro National University, Ukraine;
2I. Horbachevsky Ternopil State Medical University, Ukraine;
e-mail: hnkafor@yahoo.com

In this research, we investigated the effect of pituitrin-izadrin induced injury on the levels of metallothionein (MT) and soluble and filament forms of glial fibrillary acidic protein (GFAP) in the hippocampus, cerebellum, thalamus, and the cerebral cortex, and examined the effect of corvitin on the brain under the noted changes. Our results showed oppositely directed changes – a decrease in the level of MT and an increase in GFAP in the rat brain, with a tendency to astrogliosis development, under the influence of systemic deficiencies in myocardial function. The use of corvitin at a dose of 42 mg/kg for five days after a cardiac attack caused by pituitary-izadrin leads to recovery in the balance of the studied proteins.

Population-related peculiarities of molecular stress-responsive systems of bivalve mollusk under the effect of tetrazine pesticide

H. I. Falfushynska, L. L. Gnatyshyna, O. B. Stoliar

Volodymyr Hnatiuk Ternopil National Pedagogical University, Ukraine;
e-mail: halynka.f@gmail.com, oksana.stolyar@gmail.com

Tetrazine pesticides are widely used for the treatment of crops in most EU countries and USA. However, data about the effect of environmentally realistic concentrations of biocides on the molecular stress response system in non-target organisms are absent. The aim of our study was the comparison of adaptive capability of bivalve mollusk Anodonta cygnea from two populations under the effects of commercial pesticide Apollo in terms of biochemical parameters of the digestive gland. The differences between parameters of oxidative stress and glutathione transferase activity in specimens of control groups from clean (I group) and polluted (B group) areas have been shown. Under the effect of Apollo, the level of protein carbonyls and  microsomal oxidation processes increased, and the level of metallothioneins and oxyradical formation decreased in the specimens from both populations. However, the treatment provoked the activation of antioxidant processes in the I group and their inhibition in B group. Potentially the injury of cellular thiols, glutathione and metallothioneins, seems to be key point of tetrazine pesticides toxicity.

Manifestations of oxidative stress and molecular damages in ovarian cancer tissue

H. I. Falfushynska1,2, L. L. Gnatyshyna1,2, H. V. Deneha1, O. Y. Osadchuk1, O. B. Stoliar1

1Volodymyr Hnatiuk Ternopil National Pedagogical University, Ukraine;
e-mail: halynka.f@gmail.com;
2I.Ya. Horbachevsky Ternopil State Medical University, Ukraine

Indices of oxidative stress are recognized molecular markers and prognostic criteria for malignant transformation of tissue, but their value depends on the type of tumor and the stage of its development. The goal of this study was to clarify the relationship between the characteristics of the oxidative stress system including­ metal-associated ones and the cytotoxicity manifestations in neoplastically transformed human ovarian tissue. The highest level of Mn-superoxide dismutase activity (by 630%) and metallothionein protein (MT, 100%) has been estimated for the first time in malignant ovarian tissue compared to normal ovarian tissue. The researchers have also found a much higher level of oxy-radical formation (by 332%), a lower activity of catalase (by 49%) and a lower level of reduced glutathione (by 46%) and its redox index (0.84 versus 0.89 in the control) in tumor tissue. Under the relatively stable content of zinc, copper and cadmium in MTs, the content of zinc and especially copper in a form non-binding with MTs was significantly lower in the malignant tissue compared to normal one while the content of cadmium was higher. A discriminant analysis of all definable parameters revealed that the higher content of the products of oxidative destruction of proteins, lipids, fragmented DNA and the activity of cathepsin D, especially in its free form (by 235%), are the main characteristic signs of malignant ovarian tissue.