Tag Archives: Mytilus galloprovincialis
Fatty acids composition of actinobacteria isolated from mussels Mytilus galloprovincialis of the Black Sea Odesa Bay
N. V. Korotaeva*, K. S. Potapenko, Z. Yu. Sergieieva,
N. Yu. Vasylieva, V. O. Ivanytsia
Odesa National I. I. Mechnykov University, Odesa, Ukraine;
*e-mail: korotaeva.n@onu.edu.ua
Received: 11 December 2022; Revised: 16 March 2023;
Accepted: 13 April 2023; Available on-line: 27 April 2023
Analysis of the fatty acid composition of total cellular lipids is important chemotaxonomic characteristic that is used to identify microorganisms, in particular actinobacteria, with the help of fatty acid spectra libraries. Actinobacteria are well known as producers of secondary metabolites, which are of pharmacological and commercial interest. A significant number of actinomycetes is associated with various marine benthic communities. The aim of this study was determination of the fatty acid composition and preliminary identification of actinobacteria isolated from mussels Mytilus galloprovincialis of the Black Sea Odesa Bay. Actinobacteria of 12 isolated strains were grown in a liquid medium at 28° C for 72 h. Methyl esters of fatty acids were determined on a gas chromatograph Agilent. The MIDI Sherlock microorganisms identification system was used to identify the studied strains. It was found that the fatty acids methyl esters profiles of studied actinobacteria strains were characterized by the predominance of 12-17 saturated branched-chain fatty acids with high content of 12-methyltridecanoic, 12-methyltetradecanoic, 14-methylpentadecanoic and 14-methylhexadecanoic fatty acids. All 12 actinobacteria strains isolated from mussels (Mytilus galloprovincialis) of Odesa Bay were identified as members of the Streptomyces genus.
Distribution and polymorphism of enzymes involved in antioxidant protection and xenobiotics biotransformation in the mediterranean mussel Mytilus galloprovincialis
V. А. Toptikov, I. Yu. Chubyk*, S. V. Chebotar
Odesa National Mechnуkov University, Ukraine;
*e-mail: i.chubyk@onu.edu.ua
Received: 18 July 2022; Revised: 17 October 2022;
Accepted: 04 November 2022; Available on-line: 14 November 2022
The aim of the work was to determine the tissue distribution, activity and polymorphism of 13 enzymes involved in antioxidative protection and xenobiotics biotransformation in a five bivalve mussel organs (hepatopancreas, ctenidia, mantle, leg, adductor muscles). Iso-allozyme analysis was performed by electrophoresis, correlation relationships between the studied enzymes in the whole mussel body were carried out using Spearman’s correlation analysis. It was found that all studied enzymes are polymorphic, each organ differed in the level of enzyme activity and a set of multiple forms. The coordinated functioning of protective enzymes in various mussel organs was demonstrated.