Tag Archives: nuclear polyhedrosis virus

The search of miRNA genes in Bombyx mori nuclear polyhedrosis virus genomes regions complementary to the latest genes

T. V. Shirina, M. T. Bobrovskaja, E. A. Kozlov

Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv;
e-mail: e.a.kozlov@imbg.org.ua

The search of miRNA genes in Bombyx mori nuclear polyhedrosis virus genome region complementary to very late genes has been carried out. The search miRNA algorithm in silico was developed by us. It was shown that NPV B. mori genome region containing orf4 gene complementary to ph gene encodes the potential miRNA. NPV B. mori genome region containing p74 gene complementary to p10 gene encodes mature miRNA and potential miRNA. The genome region containing orf1629 encodes two small non-coding RNAs complementary to orf 5′-end of polyhedrin miRNA. From obtained results it is proposed that two small non-coding RNAs complementary to regions of polyhedrin miRNA are included in polyhedral.

The search of microRNA genes encoded in antiparallel chains of Autographa californica nuclear polyhedrosis virus genome regions, including most late and complementary genes

T. V. Shirina, M. T. Bobrovskaja, E. A. Kozlov

Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv;
e-mail: e.a.kozlov@imbg.org.ua

It was shown using bioinformatic approach by analysis of alternative transcriptes secondary structure, that А. californica nuclear polyhedrosis virus gene ph encoded two mature miRNAs and three potential miRNAs. Gene orf1629 complementary to gene ph did not encode miRNAs and pre-miRNA-Cs. Gene p10 encodes mature  and potential miRNA. Gene p74 located on complementary chain encodes three mature miRNAs.

RNP-complex stoichiometry of Bombyx mori nuclear polyhedrosis virus polyhedra

T. V. Shirina, M. T. Bobrovskaja, E. A. Kоzlov

Institute of Molecular Biology and Genetics,
National Academy of Sciences of Ukraine, Kyiv;
e-mail: e.a.kozlov@imbg.org.ua

By gel-filtration through Sephacryl S-300 it was shown that RNP A complex present in polyhedra of Bombyx mori nuclear polyhedrosis virus has molecular weight (Mw) about 700 kDa. It was shown that RNP A with Mw 788 kDa is composed of two polyhedrin 13S-associates with Mw 342 kDa, two p14 polypeptide with Mw 14 kDa, two 21 kDa small non-coded RNAs and two 17 kDa small non-coded RNAs. The model of RNP A formation from components making it is proposed. The complex role in the course of polyhedron formation and its role in the course of infection are discussed.

Identification of small RNA in polyhedra of Bombyx mori nuclear polyhedrosis virus

T. V. Shirina, G. V. Gerashchenko, M. T. Bobrovskaja, V. I. Kashuba

Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv;
e-mail: t.v.shirina@inbox.ru

It has been shown by bioinformatic methods­ that regions of the Bombyx mori viral nuclear poly­hedrosis genome encoded two small RNA – snc RNA-1 and snc RNA-2, which could perform a structural function in polyhedra crystals formation. The aim of this work was identification of the nucleo­tide sequence of small non-coding RNAs, predicted by bioinformatic methods in B. mori polyhedra. The following methods have been used: polymera­se chain reaction, agarose gel electrophoresis, the cloning­ of PCR products, sequencing.
There were first determined nucleotide sequences of snc RNA-1 and snc RNA-2 of polyhedrin mRNA complementary regions which are included in B. mori polyhedra. These RNAs have 100% identity with bioinformatic predicted sequences. These results confirmed our bioinformatic approach to the search for small RNAs encoded in B. mori nuclear polyhedrosis virus genome.