Ukr.Biochem.J. 2012; Volume 84, Issue 1, Jan-Feb, pp. 60-66
RNP-complex stoichiometry of Bombyx mori nuclear polyhedrosis virus polyhedra
T. V. Shirina, M. T. Bobrovskaja, E. A. Kоzlov
Institute of Molecular Biology and Genetics,
National Academy of Sciences of Ukraine, Kyiv;
e-mail: e.a.kozlov@imbg.org.ua
By gel-filtration through Sephacryl S-300 it was shown that RNP A complex present in polyhedra of Bombyx mori nuclear polyhedrosis virus has molecular weight (Mw) about 700 kDa. It was shown that RNP A with Mw 788 kDa is composed of two polyhedrin 13S-associates with Mw 342 kDa, two p14 polypeptide with Mw 14 kDa, two 21 kDa small non-coded RNAs and two 17 kDa small non-coded RNAs. The model of RNP A formation from components making it is proposed. The complex role in the course of polyhedron formation and its role in the course of infection are discussed.
Keywords: Bombyx mori, gel-filtration, nuclear polyhedrosis virus, polyhedrin, RNP-complex, small RNA
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