Tag Archives: polyhedrin

RNP-complex stoichiometry of Bombyx mori nuclear polyhedrosis virus polyhedra

T. V. Shirina, M. T. Bobrovskaja, E. A. Kоzlov

Institute of Molecular Biology and Genetics,
National Academy of Sciences of Ukraine, Kyiv;
e-mail: e.a.kozlov@imbg.org.ua

By gel-filtration through Sephacryl S-300 it was shown that RNP A complex present in polyhedra of Bombyx mori nuclear polyhedrosis virus has molecular weight (Mw) about 700 kDa. It was shown that RNP A with Mw 788 kDa is composed of two polyhedrin 13S-associates with Mw 342 kDa, two p14 polypeptide with Mw 14 kDa, two 21 kDa small non-coded RNAs and two 17 kDa small non-coded RNAs. The model of RNP A formation from components making it is proposed. The complex role in the course of polyhedron formation and its role in the course of infection are discussed.

Identification of small RNA in polyhedra of Bombyx mori nuclear polyhedrosis virus

T. V. Shirina, G. V. Gerashchenko, M. T. Bobrovskaja, V. I. Kashuba

Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv;
e-mail: t.v.shirina@inbox.ru

It has been shown by bioinformatic methods­ that regions of the Bombyx mori viral nuclear poly­hedrosis genome encoded two small RNA – snc RNA-1 and snc RNA-2, which could perform a structural function in polyhedra crystals formation. The aim of this work was identification of the nucleo­tide sequence of small non-coding RNAs, predicted by bioinformatic methods in B. mori polyhedra. The following methods have been used: polymera­se chain reaction, agarose gel electrophoresis, the cloning­ of PCR products, sequencing.
There were first determined nucleotide sequences of snc RNA-1 and snc RNA-2 of polyhedrin mRNA complementary regions which are included in B. mori polyhedra. These RNAs have 100% identity with bioinformatic predicted sequences. These results confirmed our bioinformatic approach to the search for small RNAs encoded in B. mori nuclear polyhedrosis virus genome.