Tag Archives: small intestine

The influence of novel 4-thiazolidinone derivaties in cytoprotective mechanisms of small intestine under nsaid-induced damage

I. I. Ilkiv, R. B. Lesyk, O. Ya. Sklyarov

Danylo Halytsky Lviv National Medical University, Lviv, Ukraine;
e-mail: ira9ilkiv@gmail.com

The aim of investigation was to compare the action of novel 4-thiazolidinone derivaties (compounds Les-5054 and Les-5055) toward parameters of nitroso-oxidative processes in mucous membrane of small intestine (MMSI) in rats on the background of indomethacin induced injury. The activity of nitric oxide synthases, myelopeoxidase, content of NO, and parameters of lipoperoxidation processes were measured in MMSI and the level of H2S and L-arginine in blood serum. Administration of indomethacin caused significant destructive damages in distal part of small intestine and increase in activity of inducible nitric oxide synthase (iNOS) and intensity of lipoperoxidation  processes in comparison to control were observed. Also indomethacin injection was accompanied by decrease of H2S and L-arginine level in blood serum. Administration of 4-thiazolidinone derivaties on the background of indomethacin induced injury reduce the activity of iNOS, myeloperoxidase, intensity of lipid peroxidation and increase generation of H2S, that may be linked with the structure of this compounds. However compound Les-5054 showed more significant cytoprotective effect and antioxidant properties than compound Les-5055. Thus, the novel 4-thiazolidinone derivaties led to reduce of nitroso-oxidative processes caused by administration of NSAIDs.

The effect of ionizing radiation with low dose rate on the state of electron transfer chain of enterocyte mitochondria of rat small intestine

L. V. Grubska1, V. M. Voitsitskiy2, S. V. Khyzhnayk3

1Іnstitute of Bioorganic Chemistry and Petrochemistry, National Academy of Sciences of Ukraine, Kyiv;
2National University of Life and Environmental Sciences of Ukraine, Kyiv;
3Taras Shevchenko Kyiv National University, Ukraine;
е-mail: hsv@univ.kiev.ua

The influence of ionizing radiation with low absorbed dose rate (55 mGy·min-1) in 1, 12 and 24 hours after irradiation in doses of 0.1; 0.5 and 1.0 Gy on functional state of the electron transfer  chain of the rat small intestine mitochondria was investigated by assessment of the oxygen consumption rate. The uncoupling of oxidation and phosphorylation, a decrease of phosphorylation rate and inhibition of ATP hydrolysis reactions were established in mitochondria in dependence on the irradiation dose and time interval after irradiation. The functional peculiarities of the oxidation-phosphorylation coupling sites of the mitochondrial electron transfer chain were detected.