Ukr.Biochem.J. 2015; Volume 87, Issue 1, Jan-Feb, pp. 127-133


Role of Ca ions in the induction of heat-resistance of wheat coleoptiles by brassinosteroids

Yu. E. Kolupaev1, A. A. Vayner1, T. O. Yastreb1, A. I. Oboznyi1, V. A. Khripach2

1V. V. Dokuchaev Kharkiv National Agrarian University, Ukraine;
2Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus;

The involvement of Ca2+ into the signal transduction of exogenous brassinosteroids (BS) (24-epibrassinolide – 24-EBL and 24-epicastasterone – 24 ECS) causing the increase of heat resistance of the cells of wheat (Triticum aestivum L.) coleoptiles was investigated using calcium chelator EGTA and inhibitor of phosphatidylinositol-specific phospholipase C – neomycin. Twenty-four-hour treatment of coleoptile segments with 10 nM solutions of 24-EBL and 24-ECS led to a transient increase in the generation of superoxide anion radical by cell surface and the subsequent activation of superoxide dismutase and catalase. Pretreatment of coleoptiles with EGTA and neomycin depressed to a considerable extent these effects and leveled the increase in heat resistance of wheat coleoptiles that were caused by BS. Possible mechanisms of involvement of calcium signaling into the formation of reactive oxygen species in plant cells and induction of heat resistance of plant cells by the action of exogenous BS have been discussed.

Keywords: , , , , ,


  1. Khripach V, Zhabinskii V, De Groot A. Twenty years of brassinosteroids: Steroidal plant hormones warrant better crops for the XXI century. Ann. Bot. 2000;86:441-447.
  2. Bajguz A, Hayat S. Effects of brassinosteroids on the plant responses to environmental stresses. Plant Physiol Biochem. 2009 Jan;47(1):1-8. Review. PubMed, CrossRef
  3. Dhaubhadel S, Chaudhary S, Dobinson KF, Krishna P. Treatment with 24-epibrassinolide, a brassinosteroid, increases the basic thermotolerance of Brassica napus and tomato seedlings. Plant Mol Biol. 1999 May;40(2):333-42. PubMed
  4. Singh I., Shono M. Physiological and molecular effects of 24-epibrassinolide, a brassinosteroid on thermotolerance of tomato. Plant Growth Regul. 2005;47:111-119.
  5. Divi UK, Rahman T, Krishna P. Brassinosteroid-mediated stress tolerance in Arabidopsis shows interactions with abscisic acid, ethylene and salicylic acid pathways. BMC Plant Biol. 2010 Jul 19;10:151. PubMed, PubMedCentral, CrossRef
  6. Mazorra LM, Holton N, Bishop GJ, Núñez M. Heat shock response in tomato brassinosteroid mutants indicates that thermotolerance is independent of brassinosteroid homeostasis. Plant Physiol Biochem. 2011 Dec;49(12):1420-8. PubMed, CrossRef
  7. Ogweno JO, Song XS, Shi K, Hu WH, Mao WH, Zhou YH, Yu JQ., Nogues S. Brassinosteroids alleviate heat-induced inhibition of photosynthesis by increasing carboxylation efficiency and enhancing antioxidant systems in Lycopersicon esculentum. J. Plant Growth Regul. 2008;27:49-57.
  8. Hayat S, Hasan SA, Yusuf M, Hayat Q, Ahmad A. Effect of 28-homobrassinolide on photosynthesis, fluorescence and antioxidant system in the presence or absence of salinity and temperature in Vigna radiate. Environ. Exp. Bot. 2010;69:105-112.
  9. Xia XJ, Wang YJ, Zhou YH, Tao Y, Mao WH, Shi K, Asami T, Chen Z, Yu JQ. Reactive oxygen species are involved in brassinosteroid-induced stress tolerance in cucumber. Plant Physiol. 2009 Jun;150(2):801-14. PubMed, PubMedCentral, CrossRef
  10. Cui JX, Zhou YH, Ding JG, Xia XJ, Shi K, Chen SC, Asami T, Chen Z, Yu JQ. Role of nitric oxide in hydrogen peroxide-dependent induction of abiotic stress tolerance by brassinosteroids in cucumber. Plant Cell Environ. 2011 Feb;34(2):347-58. PubMed, CrossRef
  11. Vayner AA, Kolupaev YuE, Yastreb TO, Khripach VA. The participation of reacti­ve oxygen species in the induction of thermotolerance of wheat coleoptiles caused by exogenous brassinosteroids. Bull. Kharkiv Nat. Agr. Univ. Ser. Biol. 2013;(Is. 3(30)):39-45. (In Russian).
  12. Ogasawara Y, Kaya H, Hiraoka G, Yumoto F, Kimura S, Kadota Y, Hishinuma H, Senzaki E, Yamagoe S, Nagata K, Nara M, Suzuki K, Tanokura M, Kuchitsu K. Synergistic activation of the Arabidopsis NADPH oxidase AtrbohD by Ca2+ and phosphorylation. J Biol Chem. 2008 Apr 4;283(14):8885-92. PubMed, CrossRef
  13. Ilkovets I. M., Sokolovskii S. G., Nait M. R., Volotovskii I. D. Phytohormonal control of the concentration of ionized Ca2+ in the cytoplasm of plant cell. Vesti NAN Belarusi. Ser. Biol. Navuk. 1999;(3):58-62. (In Russian).
  14. Shorning BY, Smirnova EG, Yaguzhinsky LS, Vanyushin BF. Necessity of superoxide production for development of etiolated wheat seedlings. Biochemistry (Mosc). 2000 Dec;65(12):1357-61. PubMed
  15. Karpets Yu. V., Kolupaev Yu. Ye., Shvidenko M. V. Retardation of cell death process in segments of wheat coleoptiles incubated on sucrose solution. Fiziologiya i Biokhimia Kulturnykh Rastenii. 2011;43(6):513-519. (In Russian).
  16. Kolupaev YuYe, Yastreb TO, Shvidenko MV, Karpets YuV. Influence of salicylic and succinic acids on formation of active oxygen forms in wheat coleoptiles. Ukr Biokhim Zhurn. 2011;83(5):82-88. (In Ukrainian).
  17. Kolupaev YuE, Oboznyi AI, Shvidenko NV. Role of hydrogen peroxide in generation of a signal inducing heat tolerance of wheat seedlings. Rus J Plant Physiol. 2013;60:227-234.
  18. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248-54. PubMed
  19. Liu HT, Huang WD, Pan QH, Weng FH, Zhan JC, Liu Y, Wan SB, Liu YY. Contributions of PIP2-specific-phospholipase C and free salicylic acid to heat acclimation-induced thermotolerance in pea leaves. J. Plant Physiol. 2006;163:405-416.
  20. Lee Y, Lee Y. Roles of phosphoinositides in regulation of stomatal movements. Plant Signal Behav. 2008 Apr;3(4):211-3. PubMed, PubMedCentral
  21. Lecourieux D, Mazars C, Pauly N, Ranjeva R, Pugin A. Analysis and effects of cytosolic free calcium increases in response to elicitors in Nicotiana plumbaginifolia cells. Plant Cell. 2002 Oct;14(10):2627-41. PubMed, PubMedCentral
  22. Arisz S. A., van Wijk R., Roels W., Zhu J. K., Haring M. A., Munnik T. Rapid phosphatidic acid accumulation in response to low temperature stress in Arabidopsis is generated through diacylglycerol kinase. Front. Plant Sci. 2013;4. doi: 10.3389.fpls.2013.00001.
  23. Marino D, Dunand C, Puppo A, Pauly N. A burst of plant NADPH oxidases. Trends Plant Sci. 2012 Jan;17(1):9-15. Review. PubMed, CrossRef
  24. Pappan K, Zheng S, Wang X. Identification and characterization of a novel plant phospho­lipase D that requires polyphosphoinositides and submicromolar calcium for activity in Arabidopsis. J Biol Chem. 1997;272:7048-7054.
  25. Fariduddin Q, Khalil RR, Mir BA, Yusuf M, Ahmad A. 24-Epibrassinolide regulates photosynthesis, antioxidant enzyme activities and proline content of Cucumis sativus under salt and/or copper stress. Environ Monit Assess. 2013 Sep;185(9):7845-56. PubMed, CrossRef
  26. Talaat NB, Shawky BT. 24-Epibrassinolide alleviates salt-induced inhibition of productivity by increasing nutrients and compatible solutes accumulation and enhancing antioxidant system in wheat (Triticum aestivum L.). Acta Physiol. Plant. 2013;35:729-740.
  27. Pokotylo IV, Kretynin SV, Khripach VA, Ruelland E, Blume YaB, Kravets VS. Influence of 24-epibrassinolide on lipid signalling and metabolism in Brassica napus. Plant Growth Regul. 2014;73:9-17.

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License.