Ukr.Biochem.J. 2015; Volume 87, Issue 4, Jul-Aug, pp. 54-62


Activity and isozyme content of lactate dehydrogenase under long-term oral taurine administration to rats

R. D. Ostapiv1,2, S. L. Humenyuk2, V. V. Manko1

1Ivan Franko National University of Lviv, Ukraine;
2SSRCI of Veterinary Medicinal Products and Feed Additives, Lviv, Ukraine;

The effect of long-term oral taurine administration to rats on activity of lactate dehydrogenase (LDH), its isozyme content and activity in the whole blood, liver, thigh muscle, brain and testes tissues were studied in the present work. For this purpose male Wistar rats with body weight 190–220 g were randomly divided into three groups, they were orally administered drinking water (control group) or taurine solution 40 and 100 mg per kg of body weight ( groups I and II, respectively). The total lactate dehydrogenase activity was measured spectrophotometrically, the percentage content of isozymes was determined by electrophoresis in 7.5% poliacrylamide gel with further staining according to J. Garbus. It was found that the total lactate dehydrogenase activity increased in all studied tissues. In testes of animals of both groups and in brain of group I animals, the total percentage contents of isozymes that are responsible for lactate production (LDH4+LDH5) increased. In liver of animals of both groups and in whole blood of group II animals, the total percentage content of isozymes that produce pyruvate (LDH1+LDH2) increased. In thigh muscle of both groups and in brain of group II animals the balance between LDH1+LDH2 and LDH4+LDH5 content did not differ from control values, though total lactate dehydrogenase activity was significantly higher, than that in the control group. Thus, the increase in the lactate dehydrogenase activity under long-term oral taurine administration in different rat tissues was found to be tissue- and dose-dependent and was caused by the increase in the content of different isozymes. Such increase in group I animals might be explained by adaptive mechanisms to hypoxia caused by high doses of taurine. For group II animals high doses of taurine were toxic and directly affected metabolic processes in the animal bodies.

Keywords: , , , , , , ,


  1. Kopperschläger G, Kirchberger J. Methods for the separation of lactate dehydrogenases and clinical significance of the enzyme. J Chromatogr B Biomed Sci Appl. 1996 Sep 20;684(1-2):25-49. Review. PubMed, CrossRef
  2. Lossos IS, Intrator O, Berkman N, Breuer R. Lactate dehydrogenase isoenzyme analysis for the diagnosis of pleural effusion in haemato-oncological patients. Respir Med. 1999 May;93(5):338-41. PubMed, CrossRef
  3. Wuntch T, Chen RF, Vesell ES. Lactate dehydrogenase isozymes: kinetic properties at high enzyme concentrations. Science. 1970 Jan 2;167(3914):63-5.
    PubMed, CrossRef
  4. Koukourakis MI, Giatromanolaki A, Sivridis E, Bougioukas G, Didilis V, Gatter KC, Harris AL. Lactate dehydrogenase-5 (LDH-5) overexpression in non-small-cell lung cancer tissues is linked to tumour hypoxia, angiogenic factor production and poor prognosis. Br J Cancer. 2003 Sep 1;89(5):877-85. PubMed, PubMedCentral, CrossRef
  5. Huxtable RJ. Physiological actions of taurine. Physiol Rev. 1992 Jan;72(1):101-63. Review. PubMed
  6. Ribeiro RA, Bonfleur ML, Amaral AG, Vanzela EC, Rocco SA, Boschero AC, Carneiro EM. Taurine supplementation enhances nutrient-induced insulin secretion in pancreatic mice islets. Diabetes Metab Res Rev. 2009 May;25(4):370-9. PubMed, CrossRef
  7. O’Byrne MB, Tipton KF. Taurine-induced attenuation of MPP+ neurotoxicity in vitro: a possible role for the GABA(A) subclass of GABA receptors. J Neurochem. 2000 May;74(5):2087-93. PubMed, CrossRef
  8. Vohra BP, Hui X. Improvement of impaired memory in mice by taurine. Neural Plast. 2000;7(4):245-59. PubMed, PubMedCentral, CrossRef
  9. Erdem A, Gündoğan NU, Usubütün A, Kilinç K, Erdem SR, Kara A, Bozkurt A. The protective effect of taurine against gentamicin-induced acute tubular necrosis in rats. Nephrol Dial Transplant. 2000 Aug;15(8):1175-82. PubMed, CrossRef
  10. Zhang X, Tu S, Wang Y, Xu B, Wan F. Mechanism of taurine-induced apoptosis in human colon cancer cells. Acta Biochim Biophys Sin (Shanghai). 2014 Apr;46(4):261-72. PubMed, CrossRef
  11. Aly HA, Khafagy RM. Taurine reverses endosulfan-induced oxidative stress and apoptosis in adult rat testis. Food Chem Toxicol. 2014 Feb;64:1-9. PubMed, CrossRef
  12. Levchenko VІ, Golovaha VІ, Kondrahin ІP. Methods of clinical laboratory diagnostics of animal diseases. Kyiv : Agrarna Osvita, 2010. P. 271-292. (in Ukrainian).
  13. Holod VМ, Kurdeko АP. Clinical biochemistry: tutorial in 2 tomes. Vitebsk, 2005. Vol.2. P. 20. (in Russian).
  14. Garbus J. Serum malate dehydrogenase isoenzymes as indicators of severe cellular injury. Clin Chim Acta. 1971 Dec;35(2):502-4. PubMed, CrossRef
  15. Kondrashova MN. Transaminase cycle of substrate oxidation in cell, as an adaptation mechanism to hypoxia. Pharmac Correc Hypox Stat. 1989;1:51-70.
  16. Della-Morte D, Dave KR, DeFazio RA, Bao YC, Raval AP, Perez-Pinzon MA. Resveratrol pretreatment protects rat brain from cerebral ischemic damage via a sirtuin 1-uncoupling protein 2 pathway. Neuroscience. 2009 Mar 31;159(3):993-1002. PubMedPubMedCentralCrossRef
  17. Сhance B, Williams GR.  Respiratory enzymes in oxidative phosphorilation. III. The steady state. J Biol Chem. 1955 Nov;217(1):409-27. PubMed
  18. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265-75. PubMed
  19. Derkach МP, Gumenitskiy RYa, Chaban ME. Course of variation statistics. Kyiv: Vishcha Shkola, 1977. 210 p. (in Ukrainian).
  20. El Idrissi A. Taurine increases mitochondrial buffering of calcium: role in neuroprotection. Amino Acids. 2008 Feb;34(2):321-8. PubMed, CrossRef

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