Ukr.Biochem.J. 2013; Volume 85, Issue 2, Mar-Apr, pp. 84-92

doi: http://dx.doi.org/10.15407/ubj85.02.084

The composition of lipids and lipid peroxidation in the pancreas of quails under nitrate actions and correction by the amaranth’s seeds

07.10.2015   Uncategorized   No comments

S. I. Tsekhmistrenko, N. V. Ponomarenko

Bila Tserkva State Agrarian University, Ukraine;
e-mail: ponomarenkon@ukr.net

Researches of features of lipid composition, functioning of the system of antioxidant defense, maintenance of lipid peroxidation products in the quail’s pancreas on the early postnatal ontogenesis stages are conducted for actions of nitrates and feeding with amaranth’s seeds in mixed fodder. The arrival of nitrates in the organism of quails results in the decline of general lipids maintenance and nonetherified fat acids in the pancreas. Using­ of amaranth’s seeds in mixed fodder on the background of the nitrate loading results in the increase of activity of the enzimes system of antioxidant defence, the growth of general lipid level in the quail’s pancreas. Thus in correlation with separate classes of lipid maintenance of cholesterol goes down for certain, whereas the maintenance of triacylglycerols and ethers of cholesterol rises. The results obtained in the researches show the abili­ty of amaranth’s seeds to avert oxidative stress in quail’s pancreas under nitrates influence.

Keywords: , , , , , ,

References:

  1. Baglay OM, Murska SD, Gutyy BF, Gufriy DF. Physiological-biochemical and biotechnological ways of animal productivity increasing. Sci. Messenger Lviv Nat. Univ. Veter. Med. Biotechnol. named after S.Z. Gzhytskyj. 2011;13(4(50), Patr 2):3-11.
  2. Chapman ME, Wideman RF Jr. Evaluation of total plasma nitric oxide concentrations in broilers infused intravenously with sodium nitrite, lipopolysaccharide, aminoguanidine, and sodium nitroprusside. Poult Sci. 2006 Feb;85(2):312-20. PubMed, CrossRef
  3. Goralskyi LP, Dubich IM. Morphogenesis of the dog pancreas in postnatal ontogenesis. Visnyk of Bila Tserkva State Agrarian University. Bila Tserkva,2009;60(1):30-32.
  4. Boroday VP. Bird embryo toxicosis. Suchasne Ptakhivnytstvo. 2010;(5(90)):15.
  5. Whigham LD, Israel BA, Atkinson RL. Adipogenic potential of multiple human adenoviruses in vivo and in vitro in animals. Am J Physiol Regul Integr Comp Physiol. 2006 Jan;290(1):R190-4. PubMed, CrossRef
  6. Amaranth. Prospects of application / Ed. V.A. Gnitsevich, G.F. Korshunova, O.O. Simakova, S.K. Ildirova. M. I.Tugan-Baranovsky Donetsk National University of Economics and Trade. Donetsk: DonNUET,2002. 157 p.
  7. Kolb VG, Kamyshnikov VS. Clinical Biochemistry (Manual for physician assistant). Minsk: Belarus, 1976. P. 150-154.
  8. Thin-layer and gas-liquid chromatography of lipids (Guidance) / M.F. Stefanik, V.I. Skorokhid, O.P. Eliseeva et al. Lviv, 1985. 26 p.
  9. Chevari S, Chaba I, Sekey I. Role of superoxide dismutase in cellular oxidative processes and method of its determination in biological materials.  Lab Delo. 1985;(11):678-81. Russian. PubMed
  10. Koroliuk MA, Ivanova LI, Mayorova IG, Tokarev VE. A method of determining catalase activity. Lab Delo. 1988;(1):16-9. Russian. PubMed
  11. Moin VM. A simple and specific method for determining glutathione peroxidase activity in erythrocytes. Lab Delo. 1986;(12):724-7. Russian. PubMed
  12. Iusupova LB. Increasing the accuracy of determining the glutathione reductase activity of erythrocytes. Lab Delo. 1989;(4):19-21. Russian. PubMed
  13. Goryachkovskiy AM. Clinical Biochemistry: Reference book. Odessa: Astroprint,1998. P. 370-372.
  14. Stalnaya I.D. Determination of conjugated diene of unsaturated fatty acids / Modern methods in biochemistry. Ed. V.N. Orekhovich. M.: Meditsina,1997. P. 63-64.
  15. Romanova LA., Stalnaya ID. Determination of peroxide lipid with the help of ammonium tiocinate  / Modern methods in biochemistry. Ed. V.N. Orekhovich. M.: Meditsina, 1977. P. 64-66.
  16. Stalnaya ID, Garishvili TG. Determination of malon dialdehyde with the help of thiobarbituric acid / Modern methods in biochemistry.  Ed. V.N. Orekhovich. M.: Meditsina, 1997. P. 66-68.
  17. Gostyukhina OL, Golovina IV. Peculiarities of antioxidant defense system organization of the black sea mollusks Mytilus galloprovincialis lam. and Anadara inaequivalvis br. Ukr Biokhim Zhurn. 2012 May-Jun;84(3):31-6. Russian. PubMed
  18. Goptsiy TI. Amaranth: biology, cultivation, prospects of application, selection.  Kharkiv National Agrarian University named V.V.Dokuchaev. Kh., 1999. 272 p.
  19. Kuchmerovska ТМ, Donchenko GV, Tychonenko TM, Guzyk MM, Stavniichuk RV, Yanitska LV, Stepanenko SP, Klimenko АP. Nicotinamide influence on pancreatic cells viability.  Ukr Biokhim Zhurn. 2012 Mar-Apr;84(2):81-8. Ukrainian. PubMed
  20. Lushchak VI. Adaptive response to oxidative stress: Bacteria, fungi, plants and animals. Comp Biochem Physiol C Toxicol Pharmacol. 2011 Mar;153(2):175-90. PubMed, CrossRef
  21. Yaremchuk TS, Tsekhmistrenko SI, Ponomarenko NV. Sci Tech Bull Inst Animal Biol State Sci Res Control Inst Vet Med Prod Fodder Addit. Lviv. 2012;13(1-2):75-79.
Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License.