Tag Archives: pancreas

Oxidative stress in blood leukocytes, pro/antioxidanт status and fatty acids composition of pancreas lipids at experimental acute pancreatitis in rats

I. B. Pryvrotska1, T. M. Kuchmerovska2

1I. Нorbachevsky Ternopyl State Medical University, Ukraine;
2Palladin Institute of Biochemistry, National Academy of Science of Ukraine, Kyiv;
e-mail: irina.privrotska@yandex.uа

In an experimental model of acute pancreatitis (АP) in rats no alteration in leukocyte’s viability was found by flow cytometry as compared to control. After 1 day of AP production of reactive oxygen forms in granulocytes was increased more than 5 times, but after 3 days their level was decreased. Alterations of pro/antioxidant status and specific changes in the fatty acid composition in the pancreas were established. With the development of AP, the processes of lipids peroxidation were intensified while antioxidant system was altered, that was evidenced by inflammation in the pancreas. In these conditions, the increase of phospholipase A2 activity was accompanied by significant changes of fatty acid composition of the total lipids in the pancreas. This increased relative total content of saturated fatty acids, in particular myristic, palmitic and stearic acid increased, while the total content of polyunsaturated essential fatty acids ω-3 (linolenic, eicosapentaenoic, dokozapentayenoic, docosahexaenoic) decreased. The preparation containing ω-3 polyunsaturated fatty acids partially normalized the lipid and fatty acids composition as well as prooxidant-antioxidant system.

The composition of lipids and lipid peroxidation in the pancreas of quails under nitrate actions and correction by the amaranth’s seeds

S. I. Tsekhmistrenko, N. V. Ponomarenko

Bila Tserkva State Agrarian University, Ukraine;
e-mail: ponomarenkon@ukr.net

Researches of features of lipid composition, functioning of the system of antioxidant defense, maintenance of lipid peroxidation products in the quail’s pancreas on the early postnatal ontogenesis stages are conducted for actions of nitrates and feeding with amaranth’s seeds in mixed fodder. The arrival of nitrates in the organism of quails results in the decline of general lipids maintenance and nonetherified fat acids in the pancreas. Using­ of amaranth’s seeds in mixed fodder on the background of the nitrate loading results in the increase of activity of the enzimes system of antioxidant defence, the growth of general lipid level in the quail’s pancreas. Thus in correlation with separate classes of lipid maintenance of cholesterol goes down for certain, whereas the maintenance of triacylglycerols and ethers of cholesterol rises. The results obtained in the researches show the abili­ty of amaranth’s seeds to avert oxidative stress in quail’s pancreas under nitrates influence.

Stress-responsive systems in rat pancreas upon long-term gastric hypochlorhydria and administration of multiprobiotic “Symbiter®”

K. O. Dvorshchenko, S. Ye. Vakal, A. S. Dranitsina, S. A. Senin, L. I. Ostapchenko

Taras Shevchenko National University of Kyiv, Ukraine;
e-mail: k21037@gmail.com

The intensity of free-radical processes upon long-term omeprazole-induced hypoacidity in the rat pancreas was investigated. Significant violation of oxidative-antioxidative balance in pancreatic tissue upon gastric hypochlorhydria was established: overproduction of superoxide anion, quantitative changes of lipid functional groups, increased level of lipid peroxidation products, augmentation of xanthine oxidase, superoxide dismutase and glutathione transferase activity, as well as depletion of catalase, glutathione peroxidase activity and reduced glutathione content. The inflected expression of Cckbr gene in the rat pancreas upon these conditions was also observed, thus suggesting an increased risk of pathological changes development in the gland. Abovementioned parameters were only partially restored to control values in the case of simultaneous administration of multiprobiotic “Symbiter®” with omeprazole, thus indicating the ability of this preparation to efficiently counteract the development of oxidative damages in pancrea­tic tissues upon long-term hypoacidic conditions.