S. G. Shandrenko, M. M. Savchyk, M. P. Dmytrenko

Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
e-mail: shandrenko@biochem.kiev.ua

A method for endogenous formaldehyde (FA) level evaluation has been worked out. The method involves the administration of dimedone, which forms the stable complex with FA, and the determination of formaldimedone concentration in biological samples by the fluorescence approach. The method was tested on rat’s models of FA metabolism modulation. Animals received FA (10 mg/kg); or methylamine – substrate of FA-generating enzyme SSAO, (250 mg/kg); or semicarbazide – SSAO inhibitor, (200mg/kg). Concentration of FA bound with dimedone in the liver tissue were, correspondingly: 7.5 ± 1.5 mkg/kg; 5.4 ± 0.9 mkg/kg; 2.4 ± 0.7 mkg/kg; control – 4.2 ± 1.4 mkg/kg. Obtained data indicate, that the elaborated method gives reliable information about FA level.

Keywords: dimedone, formaldehyde, formaldimedone, methylamine, semicarbazide