Tag Archives: semicarbazide

Method for endogenous formaldehyde evaluation in animal organism

S. G. Shandrenko, M. M. Savchyk, M. P. Dmytrenko

Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
e-mail: shandrenko@biochem.kiev.ua

A method for endogenous formaldehyde (FA) level evaluation has been worked out. The method involves the administration of dimedone, which forms the stable complex with FA, and the determination of formaldimedone concentration in biological samples by the fluorescence approach. The method was tested on rat’s models of FA metabolism modulation. Animals received FA (10 mg/kg); or methylamine – substrate of FA-generating enzyme SSAO, (250 mg/kg); or semicarbazide – SSAO inhibitor, (200mg/kg). Concentration of FA bound with dimedone in the liver tissue were, correspondingly: 7.5 ± 1.5 mkg/kg; 5.4 ± 0.9 mkg/kg; 2.4 ± 0.7 mkg/kg; control – 4.2 ± 1.4 mkg/kg. Obtained data indicate, that the elaborated method gives reliable information about FA level.

Influence of semicarbazide on the peroxidation processes and Lewis carcinoma growth in mice

L. M. Petrun

Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
e-mail: petrun@biochem.kiev.ua

Effects of various doses of semicarbazide on Lewis carcinoma metastasing and peroxidation processes in С57В1 mice have been investigated. In the animals with inoculated Lewis carcinoma, the semicarbazide in the dose of 1/120 LD50 had practical influence on the tumour growth and inhibited the metastasing into mice lungs (P < 0.05). Importantly, this dose significantly inhibited the formation of free radicals and active forms of oxygen against the background of decrease of the aldehydes level that was related to the acceptor properties of the drug.