Ukr.Biochem.J. 2026; Volume 98, Issue 3, May-Jun, pp. 5-27

doi: https://doi.org/10.15407/ubj98.03.005

Nitric oxide in plants under abiotic stress: involvement in signaling networks

09.06.2026   Uncategorized

Yu. E. Kolupaev1,2*, L. I. Relina1, V. P. Kolomatska1,
M. Popirny3, I. V. Shakhov1, T. O. Yastreb1

1Yuriev Plant Production Institute, National Academy of Agrarian Sciences of Ukraine, Kharkiv;
2Poltava State Agrarian University, Poltava, Ukraine;
3National Scientific Center “Institute for Soil Science and
Agrochemistry Research named after O. N. Sokolovsky”, Kharkiv, Ukraine;
*e-mail: plant_biology@ukr.net

Received: 01 April 2026; Revised: 04 May 2026;
Accepted: 29 June 2026; Available on-line: 18 June 2026

Background. Nitric oxide (NO) is recognized as one of the most vital signaling molecules in plants, involved in the control of most physiological processes under normal and, especially, stress conditions. Objective­. This review provides data on the primary and recently discovered minor pathways of nitric oxi­de synthesis in plants, NO-induced protein post-translational modifications, NO involvement in a general signa­ling network, interaction with individual nucleotides, stress-protective action of different NO donors and meta­bolic precursors of nitric oxide, along with the potential applications of these compounds in phytobiotechnologies. Conclusions. Despite numerous unresolved questions regarding nitric oxide synthesis and signa­ling in plants, current evidence highlights its central role in plant adaptation and stress responses, as well as the considerable potential of NO donors and related compounds for phytobiotechnological applications.

Keywords: , , , , , ,

References:

  1. Razzaq A, Wani SH, Saleem F, Yu M, Zhou M, Shabala S. Rewilding crops for climate resilience: economic analysis and de novo domestication strategies. J Exp Bot. 2021;72(18):6123-6139. PubMed, PubMedCentral, CrossRef
  2. Bashir K, Todaka D, Sako K, Ueda M, Aziz F, Seki M. Chemical application improves stress resilience in plants. Plant Mol Biol. 2025;115(2):47. PubMed, PubMedCentral, CrossRef
  3. Bailey R, Benton T, Challinor A, Elliott J, Gustafson D, Hiller B, Jones A, Jahn M, Kent C, Lewis K, Meacham T, Rivington M, Robson D, Tiffin R, Wuebbles DJ. Extreme weather and resilience of the global food system. Final Project Report from the UK–US Taskforce on Extreme Weather and Global Food System Resilience. The Global Food Security programme. London, 2015. 17 p.
  4. Wheeler T, von Braun J. Climate change impacts on global food security. Science. 2013;341(6145):508-513. PubMed, CrossRef
  5. Mariyam S, Bhardwaj R, Khan NA, Sahi SV, Seth CS. Review on nitric oxide at the forefront of rapid systemic signaling in mitigation of salinity stress in plants: Crosstalk with calcium and hydrogen peroxide. Plant Sci. 2023;336:111835. PubMed, CrossRef
  6. Hossain A, Skalicky M, Brestic M, Maitra S, Ashraful Alam M, Abu Syed M, Hossain J, Sarkar S, Saha S, Bhadra P, Shankar T, Bhatt R, Kumar Chaki A, EL Sabagh A, Islam T. Consequences and mitigation strategies of abiotic stresses in wheat (Triticum aestivum L.) under the changing climate. Agronomy. 2021;11(2):241. CrossRef
  7. Syed S, Mishra A. Multi-modal priming for plant stress resilience: Emerging agents and applications. Curr Plant Biol. 2025;44:100548. CrossRef
  8. Kim JM, To TK, Matsui A, Tanoi K, Kobayashi NI, Matsuda F, Habu Y, Ogawa D, Sakamoto T, Matsunaga S, Bashir K, Rasheed S, Ando M, Takeda H, Kawaura K, Kusano M, Fukushima A, Endo TA, Kuromori T, Ishida J, Morosawa T, Tanaka M, Torii C, Takebayashi Y, Sakakibara H, Ogihara Y, Saito K, Shinozaki K, Devoto A, Seki M. Acetate-mediated novel survival strategy against drought in plants. Nat Plants. 2017;3:17097. PubMed, CrossRef
  9. Kolupaev YE, Karpets YV, Beschasniy SP, Dmitriev AP. Gasotransmitters and their role in adaptive reactions of plant cells. Cytol Genet. 2019;53:392-406. CrossRef
  10. Kolupaev YE, Karpets YV, Shkliarevskyi MA, Yastreb TO, Plohovska SH, Yemets АI, Blume YaB. Gasotransmitters in plants: Mechanisms of participation in adaptive responses. Open Agricult J. 2022;16:e187433152207050. CrossRef
  11. Gautam H, Khan S, Nidhi, Sofo A, Khan NA. Appraisal of the Role of Gaseous Signaling Molecules in Thermo-Tolerance Mechanisms in Plants. Plants (Basel). 2024;13(6):791. PubMed, PubMedCentral, CrossRef
  12. Durner J, Wendehenne D, Klessig DF. Defense gene induction in tobacco by nitric oxide, cyclic GMP, and cyclic ADP-ribose. Proc Natl Acad Sci USA. 1998;95(17):10328-10333. PubMed, PubMedCentral, CrossRef
  13. Hancock JT, Corpas FJ, Silveira NM, Kolbert Z. A brief history of nitric oxide in plants: How we got here, where we are, and where we might be going. J Plant Physiol Metabol. 2025;1(1):3.
  14. Sandalio LM, Espinosa J, Shabala S, León J, Romero-Puertas MC. Reactive oxygen species- and nitric oxide-dependent regulation of ion and metal homeostasis in plants. J Exp Bot. 2023;74(19):5970-5988. PubMed, PubMedCentral, CrossRef
  15. Kolupaev YE, Yemets AI, Yastreb TO, Blume YB. The role of nitric oxide and hydrogen sulfide in regulation of redox homeostasis at extreme temperatures in plants. Front Plant Sci. 2023;14:1128439. PubMed, PubMedCentral, CrossRef
  16. Khator K, Parihar S, Jasik J, Shekhawat GS. Nitric oxide in plants: an insight on redox activity and responses toward abiotic stress signaling. Plant Signal Behav. 2024;19(1):2298053. PubMed, PubMedCentral, CrossRef
  17. Tahjib-Ul-Arif M, Wei X, Jahan I, Hasanuzzaman M, Sabuj ZH, Zulfiqar F, Chen J, Iqbal R, Dastogeer KMG, Sohag AAM, Tonny SH, Hamid I, Al-Ashkar I, Mirzapour M, El Sabagh A, Murata Y. Exogenous nitric oxide promotes salinity tolerance in plants: A meta-analysis. Front Plant Sci. 2022;13:957735. PubMed, PubMedCentral, CrossRef
  18. Venkatesan S, Masilamani P, Janaki P, Eevera T, Sundareswaran S, Rajkumar P. Role of nitric oxide in seed biology and seed production: A review. J Appl Nat Sci. 2020;12(3):277-287. CrossRef
  19. Liu X, Gong D, Ke Q, Yin L, Wang S, Gao T. Meta-Analysis of the Effect of Nitric Oxide Application on Heavy Metal Stress Tolerance in Plants. Plants (Basel). 2023;12(7):1494. PubMed, PubMedCentral, CrossRef
  20. Singhal RK, Jatav HS, Aftab T, Pandey S, Mishra UN, Chauhan J, Chand S, Indu, Saha D, Dadarwal BK, Chandra K, Khan MA, Rajput VD, Minkina T, Narayana ES, Sharma MK, Ahmed S. Roles of nitric oxide in conferring multiple abiotic stress tolerance in plants and crosstalk with other plant growth regulators. J Plant Growth Regul. 2021;40:2303-2328. CrossRef
  21. Kolupaev YE, Yastreb TO, Dmitriev AP. Gasotransmitters as key members of the signaling network regulating stomatal response: Interaction with other molecules. Phyton-Int J Exp Bot. 2024;93(12):3151-3195. CrossRef
  22. Mishra V, Singh P, Tripathi DK, Corpas FJ, Singh VP. Nitric oxide and hydrogen sulfide: an indispensable combination for plant functioning. Trends Plant Sci. 2021;26(12):1270-1285. PubMed, CrossRef
  23. Mukherjee S, Corpas FJ. H2O2 , NO, and H2S networks during root development and signalling under physiological and challenging environments: Beneficial or toxic? Plant Cell Environ. 2023;46(3):688-717. PubMed, PubMedCentral, CrossRef
  24. Nidhi, Iqbal N, Khan NA. Polyamines Interaction with Gaseous Signaling Molecules for Resilience Against Drought and Heat Stress in Plants. Plants (Basel). 2025;14(2):273. PubMed, PubMedCentral, CrossRef
  25. Kolupaev YE, Yastreb TO, Yemets A, Blume Y. Nitric oxide functional relationships with nitrogen-containing stress metabolites: Role in plant adaptation to adverse abiotic factors. Nitric Oxide. 2025;159:126-146. PubMed, CrossRef
  26. Tortella Fuentes G, Fincheira P, Rubilar O, Leiva S, Fernandez I, Schoebitz M, Pelegrino MT, Paganotti A, Dos Reis RA, Seabra AB. Nanoparticle-Based Nitric Oxide Donors: Exploring Their Antimicrobial and Anti-Biofilm Capabilities. Antibiotics (Basel). 2024;13(11):1047. PubMed, PubMedCentral, CrossRef
  27. Ullah F, Saqib S, Khan W, Ayaz A, Batool A, Wang WY, Xiong YC. The multifaceted role of sodium nitroprusside in plants: crosstalk with phytohormones under normal and stressful conditions. Plant Growth Regul. 2024;103:453-470. CrossRef
  28. Farnese FS, Menezes-Silva PE, Gusman GS, Oliveira JA. When Bad Guys Become Good Ones: The Key Role of Reactive Oxygen Species and Nitric Oxide in the Plant Responses to Abiotic Stress. Front Plant Sci. 2016;7:471. PubMed, PubMedCentral, CrossRef
  29. Khan M, Ali S, Al Azzawi TNI, Yun BW. Nitric Oxide Acts as a Key Signaling Molecule in Plant Development under Stressful Conditions. Int J Mol Sci. 2023;24(5):4782. PubMed, PubMedCentral, CrossRef
  30. Jeandroz S, Wipf D, Stuehr DJ, Lamattina L, Melkonian M, Tian Z, Zhu Y, Carpenter EJ, Wong GK, Wendehenne D. Occurrence, structure, and evolution of nitric oxide synthase-like proteins in the plant kingdom. Sci Signal. 2016;9(417):re2. PubMed, CrossRef
  31. Gupta KJ, Kaladhar VC, Fitzpatrick TB, Fernie AR, Møller IM, Loake GJ. Nitric oxide regulation of plant metabolism. Mol Plant. 2022;15(2):228-242. PubMed, CrossRef
  32. Hancock JT, Neill SJ. Nitric Oxide: Its Generation and Interactions with Other Reactive Signaling Compounds. Plants (Basel). 2019;8(2):41. PubMed, PubMedCentral, CrossRef
  33. Corpas FJ, Barroso JB. Nitric oxide synthase-like activity in higher plants. Nitric Oxide. 2017;68:5-6. PubMed, CrossRef
  34. Kolbert Z, Barroso JB, Brouquisse R, Corpas FJ, Gupta KJ, Lindermayr C, Loake GJ, Palma JM, Petřivalský M, Wendehenne D, Hancock JT. A forty year journey: The generation and roles of NO in plants. Nitric Oxide. 2019;93:53-70. PubMed, CrossRef
  35. Saha J, Brauer EK, Sengupta A, Popescu SC, Gupta K, Gupta B. Polyamines as redox homeostasis regulators during salt stress in plants. Front Environ Sci. 2015;3:21. CrossRef
  36. Yang J, Gupta V, Carroll KS, Liebler DC. Site-specific mapping and quantification of protein S-sulphenylation in cells. Nat Commun. 2014;5:4776. PubMed, PubMedCentral, CrossRef
  37. Kokorev AI, Kolupaev YE, Karpets YV, Dyachenko AI. Participation of nitric oxide in induction of heat resistance of wheat seedlings by putrescine. Rep Natl Acad Sci Ukraine. 2020;(12):85-92. CrossRef
  38. Chen D, Shao Q, Yin L, Younis A, Zheng B. Polyamine Function in Plants: Metabolism, Regulation on Development, and Roles in Abiotic Stress Responses. Front Plant Sci. 2019;9:1945. PubMed, PubMedCentral, CrossRef
  39. Astier J, Gross I, Durner J. Nitric oxide production in plants: an update. J Exp Bot. 2018;69(14):3401-3411. PubMed, CrossRef
  40. Asgher M, Per TS, Masood A, Fatma M, Freschi L, Corpas FJ, Khan NA. Nitric oxide signaling and its crosstalk with other plant growth regulators in plant responses to abiotic stress. Environ Sci Pollut Res Int. 2017;24(3):2273-2285. PubMed, CrossRef
  41. León J, Costa-Broseta Á. Present knowledge and controversies, deficiencies, and misconceptions on nitric oxide synthesis, sensing, and signaling in plants. Plant Cell Environ. 2020;43(1):1-15. PubMed, CrossRef
  42. Gupta P, Saxena G, Gupta R. Nitrate reductase-mediated nitric oxide synthesis in shaping stress resilience in plants. J Exp Bot. 2025;76(22):6634-6656. PubMed, CrossRef
  43. Terrón-Camero LC, Rodríguez-Serrano M, Sandalio LM, Romero-Puertas MC. Nitric oxide is essential for cadmium-induced peroxule formation and peroxisome proliferation. Plant Cell Environ. 2020;43(10):2492-2507. PubMed, CrossRef
  44. Costa-Broseta Á, Castillo M, León J. Post-Translational Modifications of Nitrate Reductases Autoregulates Nitric Oxide Biosynthesis in Arabidopsis. Int J Mol Sci. 2021;22(2):549. PubMed, PubMedCentral, CrossRef
  45. Lv X, Li H, Chen X, Xiang X, Guo Z, Yu J, Zhou Y. The role of calcium-dependent protein kinase in hydrogen peroxide, nitric oxide and ABA-dependent cold acclimation. J Exp Bot. 2018;69(16):4127-4139. PubMed, PubMedCentral, CrossRef
  46. Corpas FJ, Taboada J, Sánchez-Romera B, López-Jaramillo J, Palma JM. Peroxisomal Sulfite Oxidase (SOX), an alternative source of NO in higher plants which is upregulated by H2S. Plant Physiol Biochem. 2025;225:110000. PubMed, CrossRef
  47. Zhou H, Zhou Y, Zhang F, Guan W, Su Y, Yuan X, Xie Y. Persulfidation of Nitrate Reductase 2 Is Involved in l-Cysteine Desulfhydrase-Regulated Rice Drought Tolerance. Int J Mol Sci. 2021;22(22):12119. PubMed, PubMedCentral, CrossRef
  48. Zhan N, Wang C, Chen L, Yang H, Feng J, Gong X, Ren B, Wu R, Mu J, Li Y, Liu Z, Zhou Y, Peng J, Wang K, Huang X, Xiao S, Zuo J. S-Nitrosylation Targets GSNO Reductase for Selective Autophagy during Hypoxia Responses in Plants. Mol Cell. 2018;71(1):142-154. PubMed, CrossRef
  49. Wulf T, Lutter F, Safavi-Rizi V. Modulation of GSNOR activity for improved NO homeostasis and flood resilience in plants. Plant Signal Behav. 2026;21(1):2616544. PubMed, PubMedCentral, CrossRef
  50. Montilla-Bascón G, Rubiales D, Hebelstrup KH, Mandon J, Harren FJM, Cristescu SM, Mur LAJ, Prats E. Reduced nitric oxide levels during drought stress promote drought tolerance in barley and is associated with elevated polyamine biosynthesis. Sci Rep. 2017;7(1):13311. PubMed, PubMedCentral, CrossRef
  51. Martínez-Lorente SE, Pardo-Hernández M, Martí-Guillén JM, López-Delacalle M, Rivero RM. Interaction between Melatonin and NO: Action Mechanisms, Main Targets, and Putative Roles of the Emerging Molecule NOmela. Int J Mol Sci. 2022;23(12):6646. PubMed, PubMedCentral, CrossRef
  52. Singh N, Kaur H, Yadav S, Bhatla SC. Does N-nitrosomelatonin compete with S-nitrosothiols as a long distance nitric oxide carrier in plants? Biochem Anal Biochem. 2016;5(1):262. CrossRef
  53. Kolupaev YE, Yemets A, Yastreb TO, Blume Y. Functional interaction of melatonin with gasotransmitters and ROS in plant adaptation to abiotic stresses. Front Plant Sci. 2024;15:1505874. PubMed, PubMedCentral, CrossRef
  54. Gupta KJ, Kolbert Z, Durner J, Lindermayr C, Corpas FJ, Brouquisse R, Barroso JB, Umbreen S, Palma JM, Hancock JT, Petrivalsky M, Wendehenne D, Loake GJ. Regulating the regulator: nitric oxide control of post-translational modifications. New Phytol. 2020;227(5):1319-1325. PubMed, CrossRef
  55. Arora D, Jain P, Singh N, Kaur H, Bhatla SC. Mechanisms of nitric oxide crosstalk with reactive oxygen species scavenging enzymes during abiotic stress tolerance in plants. Free Radic Res. 2016;50(3):291-303. PubMed, CrossRef
  56. Umbreen S, Lubega J, Cui B, Pan Q, Jiang J, Loake GJ. Specificity in nitric oxide signalling. J Exp Bot. 2018;69(14):3439-3448. PubMed, cr id=”https://doi.org/10.1093/jxb/ery184″]
  57. Gong B, Shi Q. Identifying S-nitrosylated proteins and unraveling S-nitrosoglutathione reductase-modulated sodic alkaline stress tolerance in Solanum lycopersicum L. Plant Physiol Biochem. 2019;142:84-93. PubMed, CrossRef
  58. Sougrakpam Y, Babuta P, Deswal R. Nitric oxide (NO) modulates low temperature-stress signaling via S-nitrosation, a NO PTM, inducing ethylene biosynthesis inhibition leading to enhanced post-harvest shelf-life of agricultural produce. Physiol Mol Biol Plants. 2023;29(12):2051-2065. PubMed, PubMedCentral, CrossRef
  59. Yun BW, Feechan A, Yin M, Saidi NB, Le Bihan T, Yu M, Moore JW, Kang JG, Kwon E, Spoel SH, Pallas JA, Loake GJ. S-nitrosylation of NADPH oxidase regulates cell death in plant immunity. Nature. 2011;478(7368):264-268. PubMed, CrossRef
  60. Shen J, Zhang J, Zhou M, Zhou H, Cui B, Gotor C, Romero LC, Fu L, Yang J, Foyer CH, Pan Q, Shen W, Xie Y. Persulfidation-based Modification of Cysteine Desulfhydrase and the NADPH Oxidase RBOHD Controls Guard Cell Abscisic Acid Signaling. Plant Cell. 2020;32(4):1000-1017. PubMed, PubMedCentral, CrossRef
  61. Bai X, Yang L, Tian M, Chen J, Shi J, Yang Y, Hu X. Nitric oxide enhances desiccation tolerance of recalcitrant Antiaris toxicaria seeds via protein S-nitrosylation and carbonylation. PLoS One. 2011;6(6):e20714. PubMed, PubMedCentral, CrossRef
  62. Holzmeister C, Gaupels F, Geerlof A, Sarioglu H, Sattler M, Durner J, Lindermayr C. Differential inhibition of Arabidopsis superoxide dismutases by peroxynitrite-mediated tyrosine nitration. J Exp Bot. 2015;66(3):989-999. PubMed, PubMedCentral, CrossRef
  63. Sehrawat A, Abat JK, Deswal R. RuBisCO depletion improved proteome coverage of cold responsive S-nitrosylated targets in Brassica juncea. Front Plant Sci. 2013;4:342. PubMed, PubMedCentral, CrossRef
  64. Rodríguez-Ruiz M, González-Gordo S, Cañas A, Campos MJ, Paradela A, Corpas FJ, Palma JM. Sweet Pepper (Capsicum annuum L.) Fruits Contain an Atypical Peroxisomal Catalase That is Modulated by Reactive Oxygen and Nitrogen Species. Antioxidants (Basel). 2019;8(9):374. PubMed, PubMedCentral, CrossRef
  65. González-Gordo S, López-Jaramillo J, Rodríguez-Ruiz M, Taboada J, Palma JM, Corpas FJ. Pepper catalase: a broad analysis of its modulation during fruit ripening and by nitric oxide. Biochem J. 2024;481(13):883-901. PubMed, CrossRef
  66. Clark D, Durner J, Navarre DA, Klessig DF. Nitric oxide inhibition of tobacco catalase and ascorbate peroxidase. Mol Plant Microbe Interact. 2000;13(12):1380-1384. PubMed, CrossRef
  67. Corpas FJ, Barroso JB, González-Gordo S, Muñoz-Vargas MA, Palma JM. Hydrogen sulfide: A novel component in Arabidopsis peroxisomes which triggers catalase inhibition. J Integr Plant Biol. 2019;61(7):871-883. PubMed, CrossRef
  68. Yang H, Mu J, Chen L, Feng J, Hu J, Li L, Zhou JM, Zuo J. S-nitrosylation positively regulates ascorbate peroxidase activity during plant stress responses. Plant Physiol. 2015;167(4):1604-1615. PubMed, PubMedCentral, CrossRef
  69. Begara-Morales JC, Sánchez-Calvo B, Chaki M, Valderrama R, Mata-Pérez C, López-Jaramillo J, Padilla MN, Carreras A, Corpas FJ, Barroso JB. Dual regulation of cytosolic ascorbate peroxidase (APX) by tyrosine nitration and S-nitrosylation. J Exp Bot. 2014;65(2):527-538. PubMed, PubMedCentral, CrossRef
  70. Aroca Á, Serna A, Gotor C, Romero LC. S-sulfhydration: a cysteine posttranslational modification in plant systems. Plant Physiol. 2015;168(1):334-342. PubMed, PubMedCentral, CrossRef
  71. Muñoz-Vargas MA, González-Gordo S, Aroca A, Romero LC, Gotor C, Palma JM, Corpas FJ. Persulfidome of Sweet Pepper Fruits during Ripening: The Case Study of Leucine Aminopeptidase That Is Positively Modulated by H2S. Antioxidants (Basel). 2024;13(6):719. PubMed, PubMedCentral, CrossRef
  72. De Brasi-Velasco S, Aroca A, Romero LC, Gotor C, Sevilla F, Jiménez A. New role for thioredoxins in plants: Implication of TRXo1 in protein depersulfidation. Redox Biol. 2025;82:103627. PubMed, PubMedCentral, CrossRef
  73. Begara-Morales JC, Sánchez-Calvo B, Chaki M, Mata-Pérez C, Valderrama R, Padilla MN, López-Jaramillo J, Luque F, Corpas FJ, Barroso JB. Differential molecular response of monodehydroascorbate reductase and glutathione reductase by nitration and S-nitrosylation. J Exp Bot. 2015;66(19):5983-5996. PubMed, PubMedCentral, CrossRef
  74. Niu L, Yu J, Liao W, Xie J, Yu J, Lv J, Xiao X, Hu L, Wu Y. Proteomic Investigation of S-Nitrosylated Proteins During NO-Induced Adventitious Rooting of Cucumber. Int J Mol Sci. 2019;20(21):5363. PubMed, PubMedCentral, CrossRef
  75. Lozano-Juste J, Colom-Moreno R, León J. In vivo protein tyrosine nitration in Arabidopsis thaliana. J Exp Bot. 2011;62(10):3501-3517. PubMed, PubMedCentral, CrossRef
  76. Wang P, Zhu JK, Lang Z. Nitric oxide suppresses the inhibitory effect of abscisic acid on seed germination by S-nitrosylation of SnRK2 proteins. Plant Signal Behav. 2015;10(6):e1031939. PubMed, PubMedCentral, CrossRef
  77. Chen S, Jia H, Wang X, Shi C, Wang X, Ma P, Wang J, Ren M, Li J. Hydrogen Sulfide Positively Regulates Abscisic Acid Signaling through Persulfidation of SnRK2.6 in Guard Cells. Mol Plant. 2020;13(5):732-744. PubMed, CrossRef
  78. Kenesi E, Kolbert Z, Kaszler N, Klement É, Ménesi D, Molnár Á, Valkai I, Feigl G, Rigó G, Cséplő Á, Lindermayr C, Fehér A. The ROP2 GTPase Participates in Nitric Oxide (NO)-Induced Root Shortening in Arabidopsis. Plants (Basel). 2023;12(4):750. PubMed, PubMedCentral, CrossRef
  79. Lin W, Shang JX, Li XY, Zhou XF, Zhao LQ. Nitric Oxide Regulates Multiple Signal Pathways in Plants via Protein S-Nitrosylation. Curr Issues Mol Biol. 2025;47(6):407. PubMed, PubMedCentral, CrossRef
  80. Valderrama R, Begara-Morales JC, Chaki M, Mata-Pérez C, Padilla MN, Barroso JB. Hydrogen peroxide (H2O2)- and nitric oxide (NO)-derived posttranslational modifications. In: Gupta D, Palma J, Corpas F. (eds.). Nitric Oxide and Hydrogen Peroxide Signaling in Higher Plants. Springer, Cham., 2019. P. 37-67. CrossRef
  81. Corpas FJ, Barroso JB. Nitro-oxidative stress vs oxidative or nitrosative stress in higher plants. New Phytol. 2013;199(3):633-635. PubMed, CrossRef
  82. Blume YB, Krasylenko YA, Demchuk OM, Yemets AI. Tubulin tyrosine nitration regulates microtubule organization in plant cells. Front Plant Sci. 2013;4:530. PubMed, PubMedCentral, CrossRef
  83. Sawa T, Zaki MH, Okamoto T, Akuta T, Tokutomi Y, Kim-Mitsuyama S, Ihara H, Kobayashi A, Yamamoto M, Fujii S, Arimoto H, Akaike T. Protein S-guanylation by the biological signal 8-nitroguanosine 3′,5′-cyclic monophosphate. Nat Chem Biol. 2007;3(11):727-735. PubMed, CrossRef
  84. Mata-Pérez C, Sánchez-Vicente I, Arteaga N, Gómez-Jiménez S, Fuentes-Terrón A, Oulebsir CS, Calvo-Polanco M, Oliver C, Lorenzo Ó. Functions of nitric oxide-mediated post-translational modifications under abiotic stress. Front Plant Sci. 2023;14:1158184. PubMed, PubMedCentral, CrossRef
  85. Nishida M, Kumagai Y, Ihara H, Fujii S, Motohashi H, Akaike T. Redox signaling regulated by electrophiles and reactive sulfur species. J Clin Biochem Nutr. 2016;58(2):91-98. PubMed, PubMedCentral, CrossRef
  86. Joudoi T, Shichiri Y, Kamizono N, Akaike T, Sawa T, Yoshitake J, Yamada N, Iwai S. Nitrated cyclic GMP modulates guard cell signaling in Arabidopsis. Plant Cell. 2013;25(2):558-571. PubMed, PubMedCentral, CrossRef
  87. Honda K, Yamada N, Yoshida R, Ihara H, Sawa T, Akaike T, Iwai S. 8-Mercapto-Cyclic GMP Mediates Hydrogen Sulfide-Induced Stomatal Closure in Arabidopsis. Plant Cell Physiol. 2015;56(8):1481-1489. PubMed, CrossRef
  88. Petřivalský M, Luhová L. Nitrated Nucleotides: New Players in Signaling Pathways of Reactive Nitrogen and Oxygen Species in Plants. Front Plant Sci. 2020;11:598. PubMed, PubMedCentral, CrossRef
  89. Chmielowska-Bąk J, Sobieszczuk-Nowicka E, Arasimowicz-Jelonek M. Nitro-oxidative nucleotide modifications in plants and associated microorganisms: signalling sensors or stress symptoms? J Exp Bot. 2025;76(13):3793-3808. PubMed, PubMedCentral, CrossRef
  90. Michal Johnson J, Reichelt M, Vadassery J, Gershenzon J, Oelmüller R. An Arabidopsis mutant impaired in intracellular calcium elevation is sensitive to biotic and abiotic stress. BMC Plant Biol. 2014;14:162. PubMed, PubMedCentral, CrossRef
  91. Gundogdu N, Turker H, Unal BT, Ozturk M. Calcium-sensing receptors and signaling cascades in plants. In: Plant Receptors in Cellular Signaling. Academic Press, 2026. P. 293-300. CrossRef
  92. Chin D, Means AR. Calmodulin: a prototypical calcium sensor. Trends Cell Biol. 2000;10(8):322-328. PubMed, CrossRef
  93. Karpov PA, Yemets AI, Blume YaB. Calmodulin in action: CaM protein kinases as canonical targets in plant cell. In: Ohme V. (ed) Calmodulin: Structure, Mechanisms and Functions. Nova Science Publishers, Inc. (USA), 2019, pp.1-38.
  94. Kim MC, Chung WS, Yun DJ, Cho MJ. Calcium and calmodulin-mediated regulation of gene expression in plants. Mol Plant. 2009;2(1):13-21. PubMed, PubMedCentral, CrossRef
  95. Mir IR, Gautam H, Anjum NA, Masood A, Khan NA. Calcium and nitric oxide signaling in plant cadmium stress tolerance: A cross talk. South Afr J Bot. 2022;150:387-403. CrossRef
  96. Karpets YuV, Kolupaev YuE, Yastreb TO.Signal mediators at induction of heat resistance of wheat plantlets by short-term heating. Ukr Biochem J. 2015;87(6): 104-112. PubMed, CrossRef
  97. Courtois C, Besson A, Dahan J, Bourque S, Dobrowolska G, Pugin A, Wendehenne D. Nitric oxide signalling in plants: interplays with Ca2+ and protein kinases. J Exp Bot. 2008;59(2):155-163. PubMed, CrossRef
  98. Mur LA, Mandon J, Persijn S, Cristescu SM, Moshkov IE, Novikova GV, Hall MA, Harren FJ, Hebelstrup KH, Gupta KJ. Nitric oxide in plants: an assessment of the current state of knowledge. AoB Plants. 2013;5:pls052. PubMed, PubMedCentral, CrossRef
  99. Gupta KJ, Hancock JT, Petrivalsky M, Kolbert Z, Lindermayr C, Durner J, Barroso JB, Palma JM, Brouquisse R, Wendehenne D, Corpas FJ, Loake GJ. Recommendations on terminology and experimental best practice associated with plant nitric oxide research. New Phytol. 2020;225(5):1828-1834. PubMed, CrossRef
  100. Acheampong A, Fetisoa MR, Bondzie-Quaye P, Huang Q. Synergistic effects of arginine and calcium on astaxanthin and lipid accumulation in Haematococcus pluvialis under high light conditions. Aquaculture. 2025;598:742039. CrossRef
  101. Singh R, Parihar P, Prasad SM. Interplay of Calcium and Nitric Oxide in improvement of Growth and Arsenic-induced Toxicity in Mustard Seedlings. Sci Rep. 2020;10(1):6900. PubMed, PubMedCentral, CrossRef
  102. Sane PV, Kumar N, Baijal M, Singh KK, Kochhar VK. Activation of nitrate reductase by calcium and calmodulin. Phytochem. 1987;26(5):1289-1291. CrossRef
  103. Gao H, Jia Y, Guo S, Lv G, Wang T, Juan L. Exogenous calcium affects nitrogen metabolism in root-zone hypoxia-stressed muskmelon roots and enhances short-term hypoxia tolerance. J Plant Physiol. 2011;168(11):1217-1225. PubMed, CrossRef
  104. Laxalt AM, Raho N, Have AT, Lamattina L. Nitric oxide is critical for inducing phosphatidic acid accumulation in xylanase-elicited tomato cells. J Biol Chem. 2007;282(29):21160-21168. PubMed, CrossRef
  105. Guo Y, Li J, Liu L, Yang W, Zhou Y, Wei C, Ma J, Zhang Y, Yang J, Liu Y, Zhang H, Yuan L, Zhang X, Li H. Nitric oxide cross-links calcium signals to enhance cold tolerance via inhibiting calmodulin expression in watermelon. Plant Physiol. 2025;198(2):kiaf243. PubMed, CrossRef
  106. Hancock JT, Veal D. Nitric oxide, other reactive signalling compounds, redox, and reductive stress. J Exp Bot. 2021;72(3):819-829. PubMed, CrossRef
  107. Singh G, Patel A, Tiwari S, Gupta D, Prasad SM. Signaling molecules hydrogen sulfide (H2S) and nitric oxide (NO): Role in microalgae under adverse environmental conditions. Acta Physiol Plant. 2022;44:68. CrossRef
  108. Gross I, Durner J. In Search of Enzymes with a Role in 3′, 5′-Cyclic Guanosine Monophosphate Metabolism in Plants. Front Plant Sci. 2016;7:576. PubMed, PubMedCentral, CrossRef
  109. Hasanuzzaman M, Bhuyan MHMB, Zulfiqar F, Raza A, Mohsin SM, Mahmud JA, Fujita M, Fotopoulos V. Reactive Oxygen Species and Antioxidant Defense in Plants under Abiotic Stress: Revisiting the Crucial Role of a Universal Defense Regulator. Antioxidants (Basel). 2020;9(8):681. PubMed, PubMedCentral, CrossRef
  110. Gill SS, Tuteja N. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem. 2010;48(12):909-930. PubMed, CrossRef
  111. Kolupaev YuE, Karpets YuV. Reactive oxygen species and stress signaling in plants. Ukr Biochem J. 2014;86(4):18-35. PubMed, CrossRef
  112. Taziun T, Kumari R, Jan S, Farooq B. Interplay of hydrogen peroxide and nitric oxide in enhancing antioxidant defense under chromium (VI) toxicity in tomato. Res Crops. 2025;26(4):659-667. CrossRef
  113. Fatima A, Husain, T, Sheo SM, Prasad M, Singh VP. Implication of nitric oxide under salinity stress: the possible interaction with other signaling molecules. J Plant Growth Regul. 2022;41:163-177. CrossRef
  114. Kaya C, Ashraf M, Ahmad P, Corpas FJ. Deciphering the interplay between redox switches and signaling networks in plant stress responses. Critical Rev Plant Sci. 2025;44(3):180-209. CrossRef
  115. Liu Q, Ding Y, Shi Y, Ma L, Wang Y, Song C, Wilkins KA, Davies JM, Knight H, Knight MR, Gong Z, Guo Y, Yang S. The calcium transporter ANNEXIN1 mediates cold-induced calcium signaling and freezing tolerance in plants. EMBO J. 2021;40(2):e104559. PubMed, PubMedCentral, CrossRef
  116. Tewari RK, Hahn EJ, Paek KY. Function of nitric oxide and superoxide anion in the adventitious root development and antioxidant defence in Panax ginseng. Plant Cell Rep. 2008;27(3):563-573. PubMed, CrossRef
  117. Yemets AI, Karpets YV, Kolupaev YuE, Blume YaB. Emerging technologies for enhancing ROS/RNS homeostasis. In: Reactive Oxygen, Nitrogen and Sulfur Species in Plants: Production, Metabolism, Signaling and Defense Mechanisms, vol. 2. John Wiley & Sons Ltd, 2019. P. 873-922. CrossRef
  118. Baxter A, Mittler R, Suzuki N. ROS as key players in plant stress signalling. J Exp Bot. 2014;65(5):1229-1240. PubMed, CrossRef
  119. Ogasawara Y, Kaya H, Hiraoka G, Yumoto F, Kimura S, Kadota Y, Hishinuma H, Senzaki E, Yamagoe S, Nagata K, Nara M, Suzuki K, Tanokura M, Kuchitsu K. Synergistic activation of the Arabidopsis NADPH oxidase AtrbohD by Ca2+ and phosphorylation. J Biol Chem. 2008;283(14):8885-8892. PubMed, CrossRef
  120. Wong HL, Pinontoan R, Hayashi K, Tabata R, Yaeno T, Hasegawa K, Kojima C, Yoshioka H, Iba K, Kawasaki T, Shimamoto K. Regulation of rice NADPH oxidase by binding of Rac GTPase to its N-terminal extension. Plant Cell. 2007;19(12):4022-4034. PubMed, PubMedCentral, CrossRef
  121. Lanteri ML, Laxalt AM, Lamattina L. Nitric oxide triggers phosphatidic acid accumulation via phospholipase D during auxin-induced adventitious root formation in cucumber. Plant Physiol. 2008;147(1):188-198. PubMed, PubMedCentral, CrossRef
  122. Karpets YV, Kolupaev YE, Yastreb TO, Dmitriev OP. Possible pathways of heat resistance induction in plant cells by exogenous nitrogen oxide. Cytol Genet. 2012;46(6):354-359. CrossRef
  123. Gohari G, Alavi Z, Esfandiari E, Panahirad S, Hajihoseinlou S, Fotopoulos V. Interaction between hydrogen peroxide and sodium nitroprusside following chemical priming of Ocimum basilicum L. against salt stress. Physiol Plant. 2020;168(2):361-373. PubMed, CrossRef
  124. Corpas FJ. Plant H2S and Transcription Factors (TFs) Interactions: Unexplored Area. Front Biosci (Landmark Ed). 2025;30(5):27736. PubMed, CrossRef
  125. Corpas FJ, Muñoz-Vargas MA, González-Gordo S, Rodríguez-Ruiz M, Palma JM. Nitric oxide (NO) and hydrogen sulfide (H2S): new potential biotechnological tools for postharvest storage of horticultural crops. J Plant Growth Regul. 2025;44:1203-1220. CrossRef
  126. Aroca A, Zhang J, Xie Y, Romero LC, Gotor C. Hydrogen sulfide signaling in plant adaptations to adverse conditions: molecular mechanisms. J Exp Bot. 2021;72(16):5893-5904. PubMed, PubMedCentral, CrossRef
  127. Corpas FJ, Taboada J, Palma JM. Integration of NO and H2S signaling in higher plants. Plant Commun. 2026;7(3):101664. PubMed, PubMedCentral, CrossRef
  128. Karpets Y, Kolupaev Y, Yastreb T, Horielova O, Shkliarevskyi M, Dmitriev O. Role of reactive oxygen and nitrogen species in the induction of heat resistance of wheat plantlets by exogenous hydrogen sulfide. Rep Natl Acad Sci Ukraine. 2019;(3):89-97. CrossRef
  129. Wang Y, Li L, Cui W, Xu S, Shen W, Wang R. Hydrogen sulfide enhances alfalfa (Medicago sativa) tolerance against salinity during seed germination by nitric oxide pathway. Plant Soil. 2012;351:107-119. CrossRef
  130. Sun YY, Wang JQ, Xiang RH, Li ZG. Key role of reactive oxygen species-scavenging system in nitric oxide and hydrogen sulfide crosstalk-evoked thermotolerance in maize seedlings. Front Plant Sci. 2022;13:967968. PubMed, PubMedCentral, CrossRef
  131. Gautam H, Fatma M, Sehar Z, Mir IR, Khan NA. Hydrogen Sulfide, Ethylene, and Nitric Oxide Regulate Redox Homeostasis and Protect Photosynthetic Metabolism under High Temperature Stress in Rice Plants. Antioxidants (Basel). 2022;11(8):1478. PubMed, PubMedCentral, CrossRef
  132. Iqbal N, Umar S, Khan NA, Corpas FJ. Nitric Oxide and Hydrogen Sulfide Coordinately Reduce Glucose Sensitivity and Decrease Oxidative Stress via Ascorbate-Glutathione Cycle in Heat-Stressed Wheat (Triticum aestivum L.) Plants. Antioxidants (Basel). 2021;10(1):108. PubMed, PubMedCentral, CrossRef
  133. Hancock JT. Hydrogen sulfide and environmental stresses. Environ Exp Bot. 2019;161:50-56. CrossRef
  134. Marvasi M. Potential use and perspectives of nitric oxide donors in agriculture. J Sci Food Agric. 2017;97(4):1065-1072. PubMed, CrossRef
  135. Wills RBH, Pristijono P, Golding JB. Nitric oxide and postharvest stress of fruits, vegetables and ornamentals. In: Khan NM, Mobin M, Mohammad F. Corpas JF. (eds). Nitric Oxide Action in Abiotic Stress Responses in Plants. Springer, Cham, 2015. P. 221-238. CrossRef
  136. Seabra AB, Oliveira HC. How nitric oxide donors can protect plants in a changing environment: what we know so far and perspectives. AIMS Mol Sci. 2016;3(4):692-718. CrossRef
  137. Oliveira C, Benfeito S, Fernandes C, Cagide F, Silva T, Borges F. NO and HNO donors, nitrones, and nitroxides: Past, present, and future. Med Res Rev. 2018;38(4):1159-1187. PubMed, CrossRef
  138. Kurhaluk N, Tkaczenko H. L-Arginine and Nitric Oxide in Vascular Regulation-Experimental Findings in the Context of Blood Donation. Nutrients. 2025;17(4):665. PubMed, PubMedCentral, CrossRef
  139. Hussein HA, Alshammari SO, Kenawy SKM, Elkady FM, Badawy AA. Grain-Priming with L-Arginine Improves the Growth Performance of Wheat (Triticum aestivum L.) Plants under Drought Stress. Plants (Basel). 2022;11(9):1219. PubMed, PubMedCentral, CrossRef
  140. Riaz M, Noreen Z, Usman S, Shah AA, Zafar F, Ashraf S, Shaffique S, Mahmoud EA, Elhindi KM, Elansary HO. Nitric oxide and arginine mitigate salt stress through physio-biochemical modulations and ions regulation in Brassica napus L. Sci Rep. 2025;15(1):33204. PubMed, PubMedCentral, CrossRef
  141. Bukhari I, Akram NA. Nitric oxide and arginine improve drought stress-induced oxidative stress tolerance in sunflower (Helianthus annuus L.) by modulating some key physio-biochemical indicators. Pak J Bot. 2026;58(1):27-40. CrossRef
  142. Kohli SK, Khanna K, Bhardwaj R, Corpas FJ, Ahmad P. Nitric oxide, salicylic acid and oxidative stress: Is it a perfect equilateral triangle? Plant Physiol Biochem. 2022;184:56-64. PubMed, CrossRef
  143. Gao Y, Qi S, Wang Y. Nitrate signaling and use efficiency in crops. Plant Commun. 2022;3(5):100353. PubMed, PubMedCentral, CrossRef
  144. Teng Z, Zheng Q, Peng Y, Li Y, Meng S, Liu B, Peng Y, Duan M, Yuan D, Zhang J, Ye N. Nitrate reductase-dependent nitric oxide production mediates nitrate-conferred salt tolerance in rice seedlings. Plant Physiol. 2025;197(3):kiaf080. PubMed, CrossRef
  145. Karpets YV, Kolupaev YE, Dmitriev AP. Induction of NO synthesis in roots of wheat plantlets and development of their heat resistance by exogenous L-arginine and nitrate. Rep Natl Acad Sci Ukraine. 2017;(7):77-84. CrossRef
  146. Matysiak K, Kierzek R, Siatkowski I, Kowalska J, Krawczyk R, Miziniak W. Effect of exogenous application of amino acids l-arginine and glycine on maize under temperature stress. Agronomy. 2020;10(6):769. CrossRef
  147. Sun Y, Miao F, Wang Y, Liu H, Wang X, Wang H, Guo J, Shao R, Yang Q. L-Arginine Alleviates the Reduction in Photosynthesis and Antioxidant Activity Induced by Drought Stress in Maize Seedlings. Antioxidants (Basel). 2023;12(2):482. PubMed, PubMedCentral, CrossRef
  148. Nasibi F, Khodashenas M, Nasibi N. Priming with L-arginine reduces oxidative damages in Carthamus tinctorius seedlings under the toxic levels of lead. J Plant Physiol Breeding. 2020;10(2):13-26.
  149. Miller MR, Megson IL. Recent developments in nitric oxide donor drugs. Br J Pharmacol. 2007;151(3):305-321. PubMed, PubMedCentral, CrossRef
  150. Hussain A, Faheem B, Jang HS, Lee DS, Mun BG, Rolly NK, Yun BW. Melatonin-Nitric Oxide Crosstalk in Plants and the Prospects of NOMela as a Nitric Oxide Donor. Int J Mol Sci. 2024;25(15):8535. PubMed, PubMedCentral, CrossRef
  151. Singh N, Jain P, Gupta S, Khurana JM, Bhatla SC. N-Nitrosomelatonin, an efficient nitric oxide donor and transporter in Arabidopsis seedlings. Nitric Oxide. 2021;113-114:50-56. PubMed, CrossRef
  152. Ponomarоva IG, Morderer YeYu. What hinders the wide use of nitrogen oxide donors to increase plant tolerance to abiotic stressors. Fìzìol Rosl Genet. 2025;57(6):463-487. CrossRef
  153. Zhou Q, Tian Y, Li X, Wu Z, Wang X, Dong S. SNP application improves drought tolerance in soybean. Sci Rep. 2023;13(1):10911. PubMed, PubMedCentral, CrossRef
  154. Wei L, Zhang J, Wei S, Wang C, Deng Y, Hu D, Liu H, Gong W, Pan Y, Liao W. Nitric oxide alleviates salt stress through protein S-nitrosylation and transcriptional regulation in tomato seedlings. Planta. 2022;256(6):101. PubMed, CrossRef
  155. Kaya C, Uğurlar F, Seth CS. Sodium nitroprusside modulates oxidative and nitrosative processes in Lycopersicum esculentum L. under drought stress. Plant Cell Rep. 2024;43(6):152. PubMed, PubMedCentral, CrossRef
  156. Shakhov IV, Yastreb TO, Sakhno TV, Kolupaev YuE. Involvement of nitric oxide in γ-aminobutyric acid-induced cellular mechanisms of wheat seedling adaptation to water deficit. Cytol Genet. 2025;59(6):580-595. CrossRef
  157. Pan C, Li X, Yao S, Luo S, Liu S, Wang A, Xiao D, Zhan J, He L. S-nitrosated proteomic analysis reveals the regulatory roles of protein S-nitrosation and S-nitrosoglutathione reductase during Al-induced PCD in peanut root tips. Plant Sci. 2021;308:110931. PubMed, CrossRef
  158. He H, Oo TL, Huang W, He LF, Gu M. Nitric oxide acts as an antioxidant and inhibits programmed cell death induced by aluminum in the root tips of peanut (Arachis hypogaea L.). Sci Rep. 2019;9(1):9516. PubMed, PubMedCentral, CrossRef
  159. Ponomarova IG, Storozhenko VO, Yukhymuk VV, Morderer YY. The effect of sodium nitroprusside on the action of herbicides, protoporphyrinogen oxidase inhibitors, and synthetic auxins. Regul Mech Biosyst. 2025;16(1):e25030. CrossRef
  160.  Seabra AB, Silveira NM, Ribeiro RV, Pieretti JC, Barroso JB, Corpas FJ, Palma JM, Hancock JT, Petřivalský M, Gupta KJ, Wendehenne D, Loake GJ, Durner J, Lindermayr C, Molnár Á, Kolbert Z, Oliveira HC. Nitric oxide-releasing nanomaterials: from basic research to potential biotechnological applications in agriculture. New Phytol. 2022;234(4):1119-1125. PubMed, CrossRef
  161. Gomes DG, Debiasi TV, Pelegrino MT, Pereira RM, Ondrasek G, Batista BL, Seabra AB, Oliveira HC. Soil Treatment with Nitric Oxide-Releasing Chitosan Nanoparticles Protects the Root System and Promotes the Growth of Soybean Plants under Copper Stress. Plants (Basel). 2022;11(23):3245. PubMed, PubMedCentral, CrossRef
  162. Silveira NM, Prataviera PJ, Pieretti JC, Pieretti JC, Seabra AB, Almeid RL, Machado EC, Ribeiro RV. Chitosan-encapsulated nitric oxide donors enhance physiological recovery of sugarcane plants after water deficit. Environ Exp Bot. 2021;190:104593. CrossRef
  163. de Souza BL, Pieretti JC, Zucareli C, Seabra AB, Oliveira HC. Long-term effects of seed priming with nanoencapsulated nitric oxide donors on the early development and drought tolerance of wheat plants. Plant Sci. 2025;356:112498. PubMed, CrossRef
  164. Cao Z, Zhou H, Kong L, Li L, Wang R, Shen W. A Novel Mechanism Underlying Multi-walled Carbon Nanotube-Triggered Tomato Lateral Root Formation: the Involvement of Nitric Oxide. Nanoscale Res Lett. 2020;15(1):49. PubMed, PubMedCentral, CrossRef
  165. Kumari A, Gupta AK, Sharma S, Jadon VS, Sharma V, Chun SC, Sivanesan I. Nanoparticles as a Tool for Alleviating Plant Stress: Mechanisms, Implications, and Challenges. Plants (Basel). 2024;13(11):1528. PubMed, PubMedCentral, CrossRef
  166. Santisree P, Bhatnagar-Mathur P, Sharma KK. NO to drought-multifunctional role of nitric oxide in plant drought: Do we have all the answers? Plant Sci. 2015;239:44-55. PubMed, CrossRef
  167. Kolbert Z, Szőllősi R, Feigl G, Kónya Z, Rónavári A. Nitric oxide signalling in plant nanobiology: current status and perspectives. J Exp Bot. 2021;72(3):928-940. PubMed, CrossRef
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