Tag Archives: hydrogen sulfide
Phenylalanine ammonia-lyase activity and content of flavonoid compounds in wheat seedlings at the action of hypothermia and hydrogen sulfide donor
Yu. E. Kolupaev1,2, E. I. Horielova1, T. O. Yastreb1, Yu. V. Popov3, N. I. Ryabchun3
1Dokuchaev Kharkiv National Agrarian University, Ukraine;
e-mail: plant_biology@ukr.net;
2Karazin Kharkiv National University, Ukraine;
3Yuryev Рlant Production Institute, National Academy of Agrarian Sciences of Ukraine, Kharkiv
At present hydrogen sulfide (H2S) is considered as one of the signal mediators in plant cells. However, its role in formation of plant resistance to low temperatures and, in particular, in regulation of secondary metabolism under stress conditions remains poorly understood. The influence of H2S donor sodium hydrosulfide (NaHS) on phenylalanine ammonia-lyase (PAL) activity and content of flavonoids in wheat seedlings at normal temperature (21 °C) and under cold hardening conditions (7 days at 3 °C) was studied. After 2 days of the hardening temperature, a transient increase in PAL activity was noted. Also, activity of the enzyme was increased by treatment of plants with 0.1 or 0.5 mM NaHS under normal temperature conditions and especially at the background of cold hardening. By themselves, the cold hardening and the action of H2S donor caused an increase in total content of flavonoids and amount of anthocyanins. With the combination of hypothermia and treatment of seedlings with NaHS, this effect enlarged and the total content of flavonoids increased by 3.8, and anthocyanins increased by 1.8 times in comparison to the control. Treatment with the H2S donor caused a decrease in content of the lipid peroxidation product malonic dialdehyde in seedlings after the action of hardening temperature, and especially after their freezing at –5 °C. Also, under the influence of NaHS, survival of hardened and unhardened seedlings after cryostress increased. It was concluded that one of the mechanisms of the positive influence of the H2S donor on resistance of wheat seedlings to hypothermia is the PAL-dependent accumulation of flavonoid compounds, which have a high antioxidant activity, and a decrease in effects of secondary oxidative stress.
Induction of plant cells heat resistance by hydrogen sulfide donor is mediated by H(2)O(2) generation with participation of NADPH oxidase and superoxide dismutase
Yu. E. Kolupaev1,2, E. N. Firsova1, Т. О. Yastreb1
1V.V. Dokuchaev Kharkiv National Agrarian University, Ukraine;
e-mail: plant_biology@ukr.net;
2V.N. Karazin Kharkiv National University, Ukraine
The participation of enzymatic systems carrying out generation and conversion of reactive oxygen species (ROS), in realization of the stress-protective effect of hydrogen sulfide (H2S) on wheat coleoptile cells was investigated. It has been shown that the treatment of isolated coleoptiles with a 100 μM hydrogen sulfide donor sodium hydrosulfide (NaHS) caused a transient enhancement of the generation of superoxide anion radical (O2•–), an increase of hydrogen peroxide content and superoxide dismutase (SOD) activity in them. The increase in ROS generation was eliminated by the inhibitor of NADPH oxidase imidazole, but not by the peroxidase inhibitor sodium azide. Treatment of coleoptiles with SOD inhibitor sodium diethyldithiocarbamate (DDC) enhanced the generation of O2•– and neutralized the effect of increasing H2O2 content induced by NaHS. One day after treatment with the H2S donor, the generation of ROS decreased to a control level, while the activity of antioxidant enzymes increased markedly and the resistance of coleoptiles to damaging heating was increased. These effects of the hydrogen sulfide donor were eliminated by coleoptiles’ treatment with inhibitors of NADPH oxidase (imidazole) and SOD (DDC). It was concluded that both NADPH oxidase, generating O2•– , and SOD, which turns it into H2O2 performing signaling functions, are involved in the formation of a signal that induces protective systems and causes an increase in heat resistance of plant cells.
The influence of novel 4-thiazolidinone derivaties in cytoprotective mechanisms of small intestine under nsaid-induced damage
I. I. Ilkiv, R. B. Lesyk, O. Ya. Sklyarov
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine;
e-mail: ira9ilkiv@gmail.com
The aim of investigation was to compare the action of novel 4-thiazolidinone derivaties (compounds Les-5054 and Les-5055) toward parameters of nitroso-oxidative processes in mucous membrane of small intestine (MMSI) in rats on the background of indomethacin induced injury. The activity of nitric oxide synthases, myelopeoxidase, content of NO, and parameters of lipoperoxidation processes were measured in MMSI and the level of H2S and L-arginine in blood serum. Administration of indomethacin caused significant destructive damages in distal part of small intestine and increase in activity of inducible nitric oxide synthase (iNOS) and intensity of lipoperoxidation processes in comparison to control were observed. Also indomethacin injection was accompanied by decrease of H2S and L-arginine level in blood serum. Administration of 4-thiazolidinone derivaties on the background of indomethacin induced injury reduce the activity of iNOS, myeloperoxidase, intensity of lipid peroxidation and increase generation of H2S, that may be linked with the structure of this compounds. However compound Les-5054 showed more significant cytoprotective effect and antioxidant properties than compound Les-5055. Thus, the novel 4-thiazolidinone derivaties led to reduce of nitroso-oxidative processes caused by administration of NSAIDs.
Influence of polymicroelement preparation Esmin on hydrogen sulfide levels and indices of pro- and antioxidant system in the rat myocardium of different age
N. V. Zaichko1, A. S. Olhovskiy1, A. V. Melnik1,
P. A. Yurchenko1, A. S. Grigorieva2, N. F. Konahovich2
1Pirogov Vinnitsa National Medical University, Ukraine;
2SI Institute of Pharmacology and Toxicology,
Academy of Medical Sciences of Ukraine;
e-mail: nzaichko@mail.ru
The influence of microelement preparation Esmin on hydrogen sulfide (H2S) levels, activity of H2S -producing enzymes and indices of pro-/antioxidant system in the myocardium of different age rats were investigated. In the process of aging the levels of H2S and activities of H2S-producing enzymes (cysteine aminotransferase, cystathionine-γ-lyase) are reduced in the myocardium; the pro-/antioxidant balance is destabilized (NADPH-oxidase activity is increased and thioredoxin reductase activity is decreased). Esmin administration effectively reduces age-related changes in the myocardium of old rats: increases H2S levels and activity of H2S-producing enzymes, restores pro-/antioxidant balance.