Tag Archives: biochemical indicators
C(60) fullerene restores gastrocnemius contractile activity in a rat model of neurogenic muscle atrophy
D. M. Nozdrenko1, M. S. Anhelov1, T. Yu. Matviienko1,
O. V. Lynchak1, O. V. Korzhyk2, Yu. M. Valetskyi3,
K. I. Bogutska1, Yu. I. Prylutskyy1*
1Taras Shevchenko National University of Kyiv, Kyiv, Ukraine;
*e-mail: prylut@ukr.net;
2Lesya Ukrainka Volyn National University, Lutsk, Ukraine;
3Municipal Institution of Higher Education
“Volyn Medical Institute”, Lutsk, Ukraine
Received: 11 March 2025; Revised: 27 March 2025;
Accepted: 25 April 2025; Available on-line: 12 May 2025
Restoration of the motor function of the musculoskeletal system impaired due to innervation damage is an important clinical problem. In the study the potential therapeutic effect of C60 fullerene application was estimated in the Wistar rat model of neurogenic muscle atrophy caused by nervus ischiadicus injury. The animals were divided into the following: control, injury, injury+С60 groups. C60 fullerene aqueous solution was administrated orally for 30 days after ischiadicus injury at a daily dose of 1 mg/kg. Biomechanical parameters of gastrocnemius muscle contraction and biochemical indices (creatinine, lactate, reduced glutathione content as well as creatine phosphokinase, lactate dehydrogenase, catalase and superoxide dismutase activity) in the blood of rats were estimated on day 30 after nerve transection. It has been found that muscle strength response in the injury+С60 group was significantly enhanced, in particular, the muscle force impulse was increased by more than 30 ± 2% compared to the injury group. The studied biochemical indices of the muscle fatigue and oxidative stress in the blood of experimental animals had a pronounced tendency to increase after the initiation of the muscle neurogenic atrophy, while under the influence of C60 fullerene they decreased compared with the injury group. In our opinion, C60 fullerene prevented significant dysfunction of the gastrocnemius muscle after neurogenic atrophy by exerting an antioxidant effect and improving its contractile activity.
Evaluation of biochemical indicators in blood plasma of rats with tetracycline-induced hepatosis and their correction by milk phospholipids
V. A. Gryshchenko1, V. V. Musiychuk1, V. O. Chernyshenko2,
O. V. Gornytska2, T. M. Platonova2
1National University of Life and Environmental Sciences of Ukraine, Kyiv;
2Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
е-mail: viktoriya_004@ukr.net
Received: 13 July 2018; Accepted: 13 December 2018
Tetracycline is a drug with direct cytotoxic action on the liver, and therefore it is widely used in pharmaceutical studies of therapeutic effectiveness of hepatoprotective preparations. The aim of the present work was to determine the biochemical indicators in blood plasma of rats with tetracycline-induced hepatosis and correction properties of milk phospholipids under tetracycline-induced hepatosis in rats. To achieve this, Wistar rats were administered 250 mg/kg of 4% tetracycline hydrochloride suspension once a day intragastrically. As the corrective therapy, 1% solution of BAS “FLP-MD” was administered in liposomal form based on milk phospholipids. Under modeled steatohepatitis, significant destructive changes were observed in the cell membranes of hepatocytes in experimental rats. It was confirmed by higher activity of transaminase (in particular, activity of АSТ increased 4 times, that of ALT 1.7 times and the AST/ALT ratio was increased 2.4 times in blood plasma). The synthesis of clotting factors in livers of animals with hepatosis was inhibited. The content of fibrinogen in blood plasma decreased by 21%, factor II (prothrombin) by 27.8%, Xa-factor by 27.9%, and protein C by 40.6%. The animals also had hypochromic anemia, azotemia and bilirubinemia. The calcium-phosphor metabolism and hyperkalemia were observed. The liposomal BAS “FLP-MD” based on milk phospholipids diminished harmful effects of tetracycline, in particular supporting blood coagulation factors’ level restoration, and also by the activity of transaminases. According to the results, it may be used in prophylactics and pharmaceutical correction of steatohepatitis.