Tag Archives: children.

Toxocariasis in children with digestive system diseases

K. T. Hlushko*, H. A. Pavlyshyn

I. Horbachevsky Ternopil National Medical University,
Department of Pediatrics No 2, Ternopil, Ukraine;
*e-mail: glushko_kt@tdmu.edu.ua

Received: 29 January 2022; Revised: 29 September 2022;
Accepted: 01 October 2022; Available on-line: 19 December 2022

Toxocariasis is common among children and causes digestive diseases. The aim of the work was to study the development of toxocariasis in children with digestive diseases. The serum levels of specific IgG to Toxocara canis and Ascaris, levels of IL-4 and TNF-α were determined in 63 children. In addition, tests for parasites and a retrospective survey in order to assess sanitation and hygiene practices were conducted. All children were divided into two groups: group I includes 19 (30.2%) patients seropositive for toxocariasis, and group II – 44 (69.8%) children without any parasites. The level of IL-4 was higher in group I (18.0 ± 6.4 pg/ml) than in group II (7.2 ± 2.0 pg/ml) (P < 0.001. While the level of TNF-α did not differ significantly between groups I (4.5 ± 2.1 pg/ml) and II (3.6 ± 1.1 pg/ml) (P > 0.05). A higher incidence of Toxocara was observed among rural residents (78.9%). Children with toxocariasis more often had soil-pica (42.1%), played with dogs (100%), and did not wash their hands (84.2%) compared to the group of uninfected children. It was noted that hygienic habits and place of living contribute to Toxocara canis infection. IL-4 levels were considerably higher in group I than in group II that can be accounted for the host immune response activation, at the same time, the levels of TNF-α did not differ. Children without toxocariasis had also increased levels of IL-4, which may be related to past helminthic infection.

Dietary intake of folate and the frequency of its deficiency in children with type 1 diabetes mellitus and healthy children

L. I. Dobrovolska*, O. R. Boyarchuk, M. I. Kinash

І. Horbachevsky Ternopil National Medical University,
Department of Children’s Diseases and Pediatric Surgery, Ternopil, Ukraine;
*e-mail: dobrovolska_li@tdmu.edu.ua

Received: 29 January 2022; Revised: 22 March 2022;
Accepted: 20 September 2022; Available on-line: 19 December 2022

Adequate folate intake is essential for a child’s growth. There is lack of information about the prevalence of this nutrient deficiency in the Ukrainian population, including children. The aim of the study was to evaluate the dietary intake of folate and determine the frequency of folate deficiency in children with Type 1 diabetes mellitus (T1D) and healthy children. Determination of folate in serum was performed by ELISA. Folate level <3 ng/ml was diagnosed as a folate deficiency. Among all observed children the folate deficiency was diagnosed in 23 (32.9%): in 6 (17.1%) patients with T1D and in 17 (48.6%) healthy children (P ≤ 0.01). The mean level of serum folate in patients with T1D was (5.09 ± 2.16) ng/ml and (3.72 ± 1.87) ng/ml in healthy children (P ≤ 0.01). The average daily intake of folate with food was (138.68 ± 70.37) µg, without difference between T1D (12.00 ± 3.51 yr.) and healthy groups (10.83 ± 3.24 yr.), and it was more than two times lower than age requirements (300 µg/day). However, it was self-reported that 15 (48.9%) children of T1D group received vitamin supplementation one time in six months, while in healthy children only 6 (17.1%) children received vitamins (P ≤ 0.01). In conclusion, the frequency of folate deficiency is high in the pediatric population. Nutrition does not provide the necessary intake of folate, which indicates the need for additional folate supplementation.

Clinical and immunological peculiarities of parasitic infections in children with digestive system disorders in Western Ukraine

K. T. Hlushko*, H. A. Pavlyshyn, K. V. Kozak

Department of Pediatrics No 2, I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine;
*e-mail: glushko_kt@tdmu.edu.ua

Received: 29 January 2021; Accepted: 23 April 2021

Diseases of the digestive system such as gastroduodenal disorders (GDD) and hepatobiliary diseases (HBD) are fairly common among children. At the same time, intestinal parasites are widespread gastrointestinal tract infectious agents. We examined 108 children with GDD (n = 54) and HBD (n = 54) (mean age 11.8 ± 4.3 years) who were treated in a children’s hospital. The following were performed: blood test for specific Ig to Ascaris lumbricoides, Toxocara canis; feces analysis for helminth eggs, Giardia cysts; and pinworm test. The serum level of interleukin-4 (IL-4) was measured in 97 of 108 children. Overall parasitic co-infections were found in 60.2% of children: giardiasis in 30.6%, toxocariasis in 17.6%, ascariasis seropositivity in 13.9% and enterobiasis in 8.3% of cases. Parasitic infections (PIs) occurred in 72.2% of the HBD group and in 48.2% of the GDD group (P = 0.01). Among the PIs only ascariasis was found more often in the HBD group (22.2%) compared with the GDD group (5.6%) (P = 0.01). The mean age of children who were positive for PI (PI+) was 9.9 ± 4.6 years; while in the PI negative (PI–) group the mean age was 12.8 ± 3.3 years (P < 0.001). In GDD the IL-4 level in PI+ group was 9.3 ± 0.9 pg/ml in the versus 6.9 ± 1.8 pg/ml in the PI‒ group (P = 0.02). Children with HBD and PI had higher IL-4 levels (14.2 ± 18.8 pg/ml) compared to those without PI (7.5 ± 2.4 pg/ml) (P = 0.03). The differing impact on the course and immune response in children depending on co-infection with PIs indicates the need for additional testing for these infections.

Investigating the connections between night eating syndrome and metabolic syndrome in children

H. A. Pavlyshyn, K. V. Kozak*, K. T. Hlushko

Department of Pediatrics No 2, I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine;
*e-mail: kozakk@tdmu.edu.ua

Received: 29 January 2021; Accepted: 23 April 2021

Eating disorders are considered to be the cause of obesity, particularly its abdominal type and metabolic syndrome (MS). Until recently, night eating syndrome (NES) and MS were documented only in the adult population, but nowadays they are also seen among children. Therefore, the aim of our study was to determine the association between NES and MS. A total of 120 overweight (18.33%) and obese (81.67%) children 10-17 years of age were examined (27 girls (22.5%) and 93 boys (77.5%)). Anthropometric and blood pressure measurements were performed for all children. Serum triglycerides, high-density lipoproteins and glucose levels were assessed. Abdominal obesity was found in 70% of cases. NES was diagnosed in 20.83% of children­. MS was found in 34.17% of participants. MS was more prevalent in the NES group (56.00% (95% CI 30.62; 93.96)), compared with the non-NES group (28.42% (95% CI 18.73; 41.35)) (P < 0.05). NES increases the risk of MS development by 3 times (OR 3.21 (95% CI 1.29‒7.94); P = 0.012). Based on these results, careful screening­ for eating behavior and especially NES should be an integral part of the examination of overweight and obese children with the aim of timely diagnosis of MS.