Tag Archives: development

Developmental diet defines metabolic traits in larvae and adult Drosophila

O. M. Strilbytska1*, U. V. Semaniuk1, N. I. Burdyliyk1, V. Bubalo2, O. V. Lushchak1,3*

1Department of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian National University, Ivano-Frankivsk, Ukraine;
2Laboratory of Experimental Toxicology and Mutagenesis, L. I. Medved’s Research Center of Preventive Toxicology, Food and Chemical Safety, MHU, Kyiv, Ukraine;
3Research and Development University, Ivano-Frankivsk, Ukraine;
*e-mail: olya_b08@ukr.net or oleh.lushchak@pnu.edu.ua

Received: 04 October 2021; Accepted: 21 January 2022

The influence of the developmental nutrition on adult metabolism and overall performance becomes a hot topic of modern evolutionary biology. We used fruit fly Drosophila melanogaster as a model and experimental nutrition media composed of different sucrose content (S) and dry yeast content (Y): 0S:2Y, 20S:2Y or 0S:5Y, 20S:5Y to show that the developmental nutrition conditions define metabolism in larvae and adults. The level of glucose, glycogen, triglycerids and total lipids in the larvae and flies body were measured with the diagnostic assay kits. We found that individuals developed on either low-yeast or high-sugar diet showed delayed developmental rate. When kept on the diets with high sucrose content the larvae and adult flies had lower weight and higher amount of lipids as energy reserves. Restriction of dry yeast content in the diet of larvae led to a decrease in glycogen storage and protein levels in larvae and adult flies. The results obtained indicate that the metabolic traits revealed in adult flies are the result of nutrition during development and may be associated with mechanisms of organisms adaptation to the developmental nutritional conditions.