Tag Archives: DNA fragmentation

Lipid peroxidation and DNA fragmentation in fresh and cryopreserved spermatozoa of men at different spermatogenesis state

T. O. Yurchuk*, O. V. Pavlovich, G. O. Gapon,
A. Y. Pugovkin, M. P. Petrushko

Institute for Problems of Cryobiology and Cryomedicine, National Academy of Sciences of Ukraine, Department of Cryobiology of Reproductive System, Kharkiv;
*e-mail: taisiya.yur@gmail.com

Received: 11 July 2021; Accepted: 17 May 2021

Cryopreservation of spermatozoa is widely used in the treatment of infertility by assisted reproductive technologies. However, the cryopreservation causes an oxidative stress which can induce pathological changes in the male gametes. The aim of the research was to evaluate lipid peroxidation (LPO) and DNA fragmentation as well as correlation between these parameters in the fresh and cryopreserved spermatozoa of men with normozoospermia or oligoasthenoteratozoospermia (OAT) and spermatozoa derived from the epididymis of men with azoospermia. The level of malondialdehyde (MDA) in a TBA test, superoxide dismutase (SOD) activity and total antioxidant activity (AOA) were assessed. DNA integrity was estimated by acridine orange staining technique. It was shown that MDA level and SOD activity were significantly higher in the fresh spermatozoa of oligoasthenoteratozoospermic group compared with the fresh spermatozoa of normozoospermic group. After cryopreservation the MDA level increased in all groups and was the highest in OAT group where the greatest AOA decline was detected. DNA fragmentation frequency was 2.6 and 4.1 times higher in the fresh and cryopreserved OAT spermatozoa respectively as compared with the fresh normozoospermic ones (7.2%). DNA fragmentation was found to be the lowest in the fresh (6.2%) and cryopreserved (5.8%) epididymal spermatozoa. After cryopreservation SOD activity in epididymal spermatozoa was lower than in  normozoaspermic. In spermatozoa of the studied groups the MDA level positively correlated with DNA fragmentation (0.79 Pearson’s correlation coefficient) both before and after cryopreservation. It is suggested that due to the detected low DNA fragmentation and LPO level in epididymal spermatozoa their use could be an alternative approach for infertility treatment by assisted reproductive technologies.

Effect of N-stearoylethanolamine on the DNA fragmentation intensity in tumour and extratumoral tissues of the human adrenal cortex

N. I. Levchuk1, V. M. Pushkarev1, O. I. Kovzun1,
A. S. Mikosha1, N. M. Gula2, M. D. Tronko1

1State Institution V. P. Komisarenko Institute of Endocrinology and Metabolism,
National Academy of Medical Sciences of Ukraine, Kyiv;
2Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
e-mail: levnataly@meta.ua

The effect of different concentrations of N-stearoylethanolamine (NSE 18:0) on fragmentation of DNA in the tumoural and extratumour tissues of the adrenal glands in vitro was studied. In this work the following types of tissue were investigated­: extratumoural tissue from patients with hormonally active tumours, benign tumour tissue (hormonally active and hormonally inactive), tissue of malignant tumours and hyperplasic tissue of the adrenal glands (Itsenko-Cushing disease). It has been established that the NSE increases the intensity of DNA fragmentation only in the tissue of hormonally inactive tumours. Benign hormonally active tumours, malignant tumours and hyperplastic tissue of the adrenal glands were resistant to the NSE. The possible mechanisms of resistance to the drug are discussed.