Tag Archives: experimental diabetes

Influence of poly(ADP-ribose)polymerase inhibitors on some parameters of oxidative stress in blood leukocytes of rats with experimental diabetes

M. M. Guzyk1, K. O. Dyakun1, L. V. Yanitska2, Т. М. Kuchmerovska1

1Palladin Institute of Biochemistry, National Academy of Science of Ukraine, Kyiv;
e-mail: kuch@biochem.kiev.ua;
2O. O. Bogomolets’ National Medical University, Kyiv, Ukraine;

The study was undertaken to investigate the influence of specific inhibitors of poly(ADP-ribose)polymerase-1 (PARP-1), in particular nicotinamide and 1,5-isoqinolinediol on white blood cells of rats with diabetes. Using the fluorescent probe 2′,7′-dichlorodihydrofluorescein diacetate ROS production in leukocytes was asseced. It was found that the development of streptozotocin-induced diabetes was accompanied by an intensification of oxidative stress and a significant decrease in via­bility of blood leukocytes as compared to control animals. Administration of PARP-1 inhibitors prevented the development of oxidative stress in leukocytes and increased their viability. It was shown a reduction of superoxide dismutase activi­ty in serum in diabetes. Investigated PARP-1 inhibitors had no effect on the activity of superoxide dismutase and glucose levels in the blood. The findings­ suggest the intensification of oxidative stress in leukocytes of diabetic animals and the ability of nicotinamide and 1,5-isoqinolinediol to prevent its development depending on the features of their structure.

The alkaloid-free fraction from Galega officinalis extract prevents oxidative stress under experimental diabetes mellitus

M. I. Lupak, M. R. Khokhla, G. Ya. Hachkova, O. P. Kanyuka,
N. I. Klymyshyn, Ya. P. Chajka, M. I. Skybitska, N. O. Sybirna

Ivan Franko National University of Lviv, Ukraine;
е-mail: sybirna_natalia@yahoo.com

The effect of alkaloid-free fraction from Galega officinalis extract on the process of formation of reactive oxygen species and indicators of prooxidant-antioxidant balance was investigated in rat peri­pheral blood under conditions of experimental diabetes mellitus. It was shown that alkaloid-free fraction from Galega officinalis extract prevents oxidative stress development in rats with streptozotocin-induced diabetes, providing antioxidant and antiradical mobilization mechanisms to protect the blood system. In the case of extract application to animals with studied­ pathology, one can observe a reducing effect of reactive oxygen species generation in leukocytes, inhibition of proteins and lipids oxidative modification processes and increased activity of key enzymes of rat peripheral blood antioxidant system (superoxide dismutase, catalase and glutathione peroxidase). The revealed biological effect could be explained by the presence of biologically active substances with antioxidant properties in the extract composition (phytol and flavonoids).