L. M. Babenko¹, T. D. Skaterna², I. V. Kosakivska¹

¹M.G. Kholodny Institute of Botany, National Academy of Sciences of Ukraine, Kyiv;
²Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
e-mail: lilia.babenko@gmail.com

We analyzed the peculiarities of lipoxygenase (LOX) localization and activity in organs of two higher vascular cryptogams at the different phenological phases of development. For the first time it was showed that floating fronds of the annual water fern Salvinia natans (L.) contained 13-LOX with рН_opt value of 8.0, when submerged ones – 13-LOX (рН_opt 8.0) at the initial phenological phases of development and 9-LOX (рН_opt 5.5) at the subsequent phases. In sporocarps 9-LOX (рН_оpt 5.5) was identified. At the same time in fronds of the perennial land fern Polystichum aculeatum (L.) Roth. we identified 13-LOX with рН_opt value of 7.74, and in the rhizome – 9-LOX (рН_opt 7.54). The localization pattern and change of LOXs isoform activity in ferns organs in various phenological phases of development allow us to suggest that enzyme may be involved in the regulation of growth processes that ensure adaptation to environmental conditions.