Tag Archives: low density lipoproteins

Potential circulating biomarkers of atherosclerotic plaque vulnerability in patients in the early recovery period of atherothrombotic stroke

O. Ya. Mykhalojko, I. Ya. Mykhalojko

Ivano-Frankivsk National Medical University, Ivano-Frankivsk, Ukraine;
e-mail: myhalojko@i.ua

Received: 30 November 2022; Revised: 02 February 2023;
Accepted: 13 April 2023; Available on-line: 27 April 2023

Despite the informative value of ultrasound examination of atherosclerotic vascular lesions, predicting­ the plaque vulnerabili­ty remains difficult. Circulating blood biomarkers could provide additional criteria that would allow better determination of the risk of recurrent stroke. The aim of our study was to estimate the level of C-reactive protein (CRP), low-density lipoproteins (LDL) and lipoprotein-associated phospholipase A2 (Lp-PLA2) in the blood of patients in the early recovery period of atherothrombotic stroke depending on the density of atherosclerotic plaque according to duplex scanning of cerebral vessels. Clinical and laboratory analysis of 69 men and 61 women aged (60.42 ± 7.40) years in the early recovery period of atherothrombotic stroke was conducted. Depending on the structure of the atherosclerotic plaque the examinees were divided into two groups with stable (n = 80) and unstable (n = 50) atherosclerotic layers. The blood lipid spectrum was examined on a biochemical analyzer Screen master, the level of CRP was determined with a diagnostic kit and that of Lp-PLA2 by ELISA. Significantly higher levels of LDL, CRP and Lp-PLA2 were observed in patients with unstable atherosclerotic plaque compared to patients with stable atherosclerotic plaque in the early recovery period of atherothrombotic stroke. The increased level of LDL, CRP, and Lp-PLA2 in patients with cerebral atherosclerosis can be considered as an indicator of the vulnerability of atherosclerotic plaques, prone to rupture, and as a prognostic marker of repeated acute ischemic events.

Evaluation of metallothioneins, oxidative stress and signs of cytotoxicity in young obese women

H. I. Falfushynska, O. I. Horyn, V. V. Khoma, G. V. Tereshchuk,
D. V. Osadchuk, N. I. Rusnak, O. B. Stoliar

Ternopil Volodymyr Hnatiuk National Pedagogical University, Ukraine;
e-mail: falfushynska@tnpu.edu.ua

Obesity is rapidly increasing all over the world and pretends to be the global medical and social problem. Thus, the understanding of early signs of obesity and suitable biomarkers is urgently needed for developing an adequate strategy of the obesity prevention and a decrease in its growth rate. The parameters of the lipids’ metabolism and oxidative stress, metallothioneins and signs of cytotoxicity have been investigated in blood samples of young obese women (O-group, 32 < Body Mass Index (BMI) < 37). With regard to persons of O-group they had higher catalase activity (by 435%), level of reactive oxygen species (by 129%), level of oxidised glutathione (by 55%), lipid peroxidation (by 26%) and protein carbonyls (by 345%) in the blood, when compared with control. The obesity was accompanied by an increase in concentration of metallothioneins which have a partial tread effect on radical processes and reduce manifestations of oxidative damage to biomolecules in obese patients. The obese women had the signs of cytotoxicity as higher lactate dehydrogenase activity (by 387%) and DNA fragmentation (by 42%). The principal component analysis revealed the set of biological traits which describes the obesity progress and it included metallothioneins, parameters of oxidative stress, cytotoxicity, BMI and a concentration of low density lipoproteins and total cholesterol. The BMI was in a good correlation with parameters of the lipid metabolism, oxidative injury and cytotoxicity (r > |0.73|, P < 0.001).