Ukr.Biochem.J. 2023; Volume 95, Issue 1, Jan-Feb, pp. 58-63


Potential circulating biomarkers of atherosclerotic plaque vulnerability in patients in the early recovery period of atherothrombotic stroke

O. Ya. Mykhalojko, I. Ya. Mykhalojko

Ivano-Frankivsk National Medical University, Ivano-Frankivsk, Ukraine;

Received: 30 November 2022; Revised: 02 February 2023;
Accepted: 13 April 2023; Available on-line: 27 April 2023

Despite the informative value of ultrasound examination of atherosclerotic vascular lesions, predicting­ the plaque vulnerabili­ty remains difficult. Circulating blood biomarkers could provide additional criteria that would allow better determination of the risk of recurrent stroke. The aim of our study was to estimate the level of C-reactive protein (CRP), low-density lipoproteins (LDL) and lipoprotein-associated phospholipase A2 (Lp-PLA2) in the blood of patients in the early recovery period of atherothrombotic stroke depending on the density of atherosclerotic plaque according to duplex scanning of cerebral vessels. Clinical and laboratory analysis of 69 men and 61 women aged (60.42 ± 7.40) years in the early recovery period of atherothrombotic stroke was conducted. Depending on the structure of the atherosclerotic plaque the examinees were divided into two groups with stable (n = 80) and unstable (n = 50) atherosclerotic layers. The blood lipid spectrum was examined on a biochemical analyzer Screen master, the level of CRP was determined with a diagnostic kit and that of Lp-PLA2 by ELISA. Significantly higher levels of LDL, CRP and Lp-PLA2 were observed in patients with unstable atherosclerotic plaque compared to patients with stable atherosclerotic plaque in the early recovery period of atherothrombotic stroke. The increased level of LDL, CRP, and Lp-PLA2 in patients with cerebral atherosclerosis can be considered as an indicator of the vulnerability of atherosclerotic plaques, prone to rupture, and as a prognostic marker of repeated acute ischemic events.

Keywords: , , , ,


  1. Monbailliu T, Goossens J, Hachimi-Idrissi S. Blood protein biomarkers as diagnostic tool for ischemic stroke: a systematic review. Biomark Med. 2017;11(6):503-512. PubMed, CrossRef
  2. Katan M, Elkind M. The potential role of blood biomarkers in patients with ischemic stroke: An expert opinion. Clin Transl Neurosci. 2018;2(1):1-7. CrossRef
  3. Jickling GC, Sharp FR. Biomarker panels in ischemic stroke. Stroke. 2015;46(3):915-920. PubMed, PubMedCentral, CrossRef
  4. Mykhalojko OJa, Mykhalojko ІJa. Some biochemical changes in patients with acute ischemic stroke. Ukr Biochem J. 2017;89(3):31-35. CrossRef
  5. Van Lammeren G, Moll F, Borst G, de Kleijn D, de Vries J, Pasterkamp G. Atherosclerotic plaque biomarkers: beyond the horizon of the vulnerable plaque. Curr Cardiol Rev. 2011;7(1):22-27. PubMed, PubMedCentral, CrossRef
  6. Ezhov MV, Safarova MS, Afanasieva OI, Kukharchuk VV, Pokrovsky SN. Lipoprotein(a) level and apolipoprotein(a) phenotype as predictors of long-term cardiovascular outcomes after coronary artery bypass grafting. Atherosclerosis. 2014;235(2):477-482. PubMed, CrossRef
  7. Han L, Zhong C, Bu X, Xu T, Wang A, Peng Y, Xu T, Wang J, Peng H, Li Q, Ju Z, Geng D, Zhang Y, He J. Prognostic value of lipoprotein-associated phospholipase A2 mass for all-cause mortality and vascular events within one year after acute ischemic stroke. Atherosclerosis. 2017;266:1-7. PubMed, CrossRef
  8. Marcovina SM, Albers JJ. Lipoprotein (a) measurements for clinical application. J Lipid Res. 2016;57(4):526-537. PubMed, PubMedCentral, CrossRef
  9. Huang F, Wang K, Shen J. Lipoprotein-associated phospholipase A2: The story continues. Med Res Rev. 2020;40(1):79-134. PubMed, PubMedCentral, CrossRef
  10. Wong ND, Zhao Y, Sung J, Browne A. Relation of First and Total Recurrent Atherosclerotic Cardiovascular Disease Events to Increased Lipoprotein(a) Levels Among Statin Treated Adults With Cardiovascular Disease. Am J Cardiol. 2021;145:12-17. PubMed, PubMedCentral, CrossRef
  11. Alkuraishy HM, Al-Gareeb AI, Waheed HJ. Lipoprotein-Associated Phospholipase A2 is Linked with Poor Cardio-Metabolic Profile in Patients with Ischemic Stroke: A Study of Effects of Statins. J Neurosci Rural Pract. 2018;9(4):496-503. PubMed, PubMedCentral, CrossRef
  12. Pu Y, Li S, Wang L, Fang B, Bai X. Association Between High-Sensitivity C-Reactive Protein and Prognosis of Patients with Acute Cerebral Infarction. Neuropsychiatr Dis Treat. 2022;18:1771-1778. PubMed, PubMedCentral, CrossRef
  13. Ladenvall C, Jood K, Blomstrand C, Nilsson S, Jern C, Ladenvall P. Serum C-reactive protein concentration and genotype in relation to ischemic stroke subtype. Stroke. 2006;37(8):2018-2023. PubMed, CrossRef
  14. Israr M, Gul C, Rehman AU, Khan AS, Siyar M, Rahim S, Arshad M. Relationship of Lipids, C-Reactive Protein and Sialic Acid in the Healthy Individuals. J Ayub Med Coll Abbottabad. 2018;30(2):213-216. PubMed

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