Tag Archives: C-reactive protein

Profiling of metabolic biomarkers in the serum of prostate cancer patients

F. Ali1, S. Akram1, S. Niaz1,2, N. Wajid1

1Institute of Molecular Biology and Biotechnology (IMBB) & Centre for Research In Molecular Medicine (CRIMM), The University of Lahore, Raiwind Road Lahore, Pakistan;
2Social Security Hospital Multan Chungi, Multan Road, Lahore;
e-mail: Fatima.ali@imbb.uol.edu.pk; fatemei.ali@gmail.com

Received: 26 July 2019; Accepted: 29 November 2019

Prostate cancer (PCa) is the major cause of the death of men population globally. Multiple factors are involved in the initiation and progression of PCa. This study aimed to evaluate different metabolic parameters in the serum of PCa patients. Males of 50 years and above age with the recent diagnosis of PCa (digital rectal examination, and elevated serum prostate-specific antigen (PSA) level) were included in the study. Glucose and serum electrolytes level, lactate dehydrogenase activity, parameters of lipid metabolism and liver and kidney functioning were measured on a fully automated analyzer using standard reagent kits. Oxidative stress was evaluated by measuring MDA, CAT, GSH, and SOD in serum. Detection of C-reactive protein (CRP), insulin-like growth factor (IGF-1) and vascular endothelial growth factor (VEGF) was performed by immunoassay. It was shown that serum glucose and HDL levels were lower while total cholesterol, LDL and triglyceride levels were significantly higher in PCa group than in the control group. PCa patients had an elevated level of liver and kidney functional markers. Comparison of the oxidative stress markers in patient and control groups showed significant difference. It was detected that serum levels of CRP, IGF-1 and VEGF were significantly higher in PCa group, compared the control to group (P < 0.05). Low level of glucose and dyslipidemia indices in prostate cancer patients indicated metabolic changes and demonstrated the importance of multiple parameters analysis (free PSA, dyslipidemia, VEGF, IGF-1, CRP, and oxidative stress markers) for early PCa diagnostics.

Correlation between base concentration of C-reactive protein in the blood, levels of serum antiendotoxin antibodies and endotoxin-binding capacity of monocytes and granulocytes of healthy people

A. I. Gordienko

State Institution S. I. Georgievsky Crimea State Medical University, Simferopol, Ukraine;
e-mail: uu4jey@csmu.strace.net

The associative links between base concentration of C-reactive protein (hsCRP), levels of serum antiendotoxin antibodies of different classes and endotoxin-binding capacity of monocytes and granulocytes of healthy volunteers were investigated­ by cluster analysis. In the group of healthy volunteers with increased base concentration of hsCRP in blood the levels of serum antiendotoxin antibodies of different  classes and endotoxin-binding capacity of monocytes and granulocytes were reduced. Thus, the disbalance of detoxification and clearance of  endotoxin by humoral and cellular mechanisms can be one of the possible causes of development of low intensity inflammation and increase of hsCRP concentration in blood.

Levels of serum antibodies to enterobacterial lipopolysaccharides and their relationship with concentration of C-reactive protein in diabetes mellitus patients

A. I. Gordienko

S. I. Georgievsky Crimea state medical university, Simferopol;
e-mail: uu4jey@csmu.strace.net

We examined patients with type 1 (DM 1) and type 2 (DM 2) diabetes mellitus. The concentration of C-reactive protein (CRP) in the blood and levels of serum antibodies to different classes of enterobacterial lipopolysaccharides (LPS) were determined by ELISA. Using cluster analysis it was shown that in 40.8% DM-1 patients the increased concentration of CRP is associated with a decrease in the levels of serum anti-LPS-IgA, anti-LPS-IgM and anti-LPS-IgG. In 56.7% of DM-2 patients with increased concentration of CRP levels of serum anti-LPS-IgA and anti-LPS-IgM were not significantly different from the normal values, but the levels of serum anti-LPS-IgG were significantly increased. Activation of inflammation and increase of concentration of the CRP in the blood of DM-2 patients is accompanied by a significant increase in the levels of serum anti-LPS-A and anti-LPS-G, as well as the tendency to reduce the levels of anti-LPS-IgM. The results of this study suggest an association between low intensity inflammation and immune response to enterobacterial LPS  in type 1 and 2 diabetes mellitus.