Tag Archives: nitrogen oxide
Pro-/antioxidant reactions and nitrogen oxide metabolism under sub-chronic effect of succinic acid derivatives
I. A. Palagina
State Institution V. Danilevsky Institute for Endocrine Pathology Problems, National Academy of Medical Science of Ukraine, Kharkiv;
e-mail: lab-tox@ukr.net
The derivatives of a succinic acid represent active compounds which realize their biological action via the influence on pro-/antioxidant processes and energetic metabolism. We studied the nitrogen oxide metabolism as well as reactions of the proteins and lipids free-radical peroxide oxidation under a sub-chronic effect of anti-diabetically active compounds – succinic acid derivatives and its metabolites. We found that the studied compounds were able to change the intensity of protein- and lipoperoxidation in a liver and blood serum interconnected with changes in the activity of the key antioxidant enzymes. The derivatives of succinic acid proved to inhibit the nitrogen oxide synthase reflected in a decrease in nitrite- and nitrate-anion level in a liver, blood plasma and urine as well as in changes in the oxidation processes in a liver and blood serum. We concluded in stating that the specified changes of pro-/antioxidant and nitrogen oxide homeostasis may serve as assessment criteria of the succinate-containing compounds’ biological action.
Asbestos-stimulated changes in nitric oxide and iron metabolism in rats
S. G. Shandrenko, T. O. Kishko, I. N. Chumachenko, N. P. Dmitrenko
Palladin Institute of Biochemistry, National Academy
of Sciences of Ukraine, Kyiv;
е-mail: dmytrenko@biochem.kiev.ua
Under intratracheal asbestos fibers installation it has been investigated NO synthesis in the lung and liver tissues of Wistar rats by EPR method. Asbestos А6-45, sifted through the sieve with size 0.1 mm, has been administrated in a dose of 5 mg/kg. To evaluate the NO synthesis EPR and NO-trap methods have been used. The amplitude of EPR signal “trap-NO” in the lung samples was 12, 16 and 14 times greater than in controls on the 3th, 6th and 10th days after asbestos installation and was corresponding to NO rate of about 2 mkmol/(g∙h). In the liver samples of asbestos-stimulated animals the NO level contained in the non-heme iron nitrosyl complexes was about 2 mkmol/g. Thus, the asbestos fibers stimulate NO synthesis not only in the lung tissue, but also in other organs. The obtained data shows that under NO hyperproduction certain changes in iron metabolism take place, such as: the decrease of transferrin iron and the accumulation of ferric iron not bound with transferrin. The accumulation of ferric iron not shielded by proteins is one of the oxidative stress triggers.