Tag Archives: peroxidation
Administration of platelet-rich plasma or concentrated bone marrow aspirate after mechanically induced ischemia improves biochemical parameters in skeletal muscle
А. Т. Pidlisetsky1, G. V. Kosiakova2, T. M. Goridko2, A. G. Berdyschev2,
O. F. Meged2, S. I. Savosko3*, О. V. Dolgopolov4
1Lviv Regional Hospital for War Veterans and Repressed named after Yu. Lypa, Ukraine;
2Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
3Bogomolets National Medical University, Kyiv, Ukraine;
4SI “Institute of Traumatology and Orthopedics of NAMS of Ukraine”, Kyiv;
*e-mail: savosko_s@ukr.net
Received: 16 September 2020; Accepted: 17 May 2021
Ischemia is known to cause structural and functional damage to skeletal muscle. In this research platelet-rich plasma (PRP) and cell bone marrow aspirate (CBMA) which are considered to be promising in regenerative medicine were used to improve the animal muscles condition after ischemia. Mechanically induced ischemia (MII) of the rabbit skeletal muscles was caused by applying a tourniquet to the hind limb for 6 hours. At 6 h after tourniquet was removed the rabbit PRP or CBMA solutions were injected into the tibia muscle and animals were divided into experimental groups (n = 15): 1 – ischemia + saline, 2 – ischemia + PRP, 3 – ischemia + CBMA. Intact animals were the control group (n = 5). Animals were removed from the experiment on 5, 15, and 30th days of reperfusion after ischemia. A progressive hypotrophy, muscle fibers destruction and leukocyte infiltration was observed after tibia muscle MII. The ischemic state was accompanied by catalase and superoxide dismutase activation, persistent NO2– overproduction and a significant decrease in glutathione peroxidase activity in the muscle homogenates. It was shown that after PRP or CBMA local injection into the ischemic muscle catalase activity remained at the elevated level, SOD activity approached the control level and the most pronounced effects were NO2– level decrease and glutathioperoxidase activity restoration. After CBMA injection angiogenesis activation and the increase in the number of nuclei in the muscle fibrils were observed. The obtained data allow to expand the range of biochemical parameters for assessing the muscles condition after ischemia and indicate a positive effect of platelet-rich plasma and cell bone marrow aspirate injection on skeletal muscle regeneration.
Pro-/antioxidant reactions and nitrogen oxide metabolism under sub-chronic effect of succinic acid derivatives
I. A. Palagina
State Institution V. Danilevsky Institute for Endocrine Pathology Problems, National Academy of Medical Science of Ukraine, Kharkiv;
e-mail: lab-tox@ukr.net
The derivatives of a succinic acid represent active compounds which realize their biological action via the influence on pro-/antioxidant processes and energetic metabolism. We studied the nitrogen oxide metabolism as well as reactions of the proteins and lipids free-radical peroxide oxidation under a sub-chronic effect of anti-diabetically active compounds – succinic acid derivatives and its metabolites. We found that the studied compounds were able to change the intensity of protein- and lipoperoxidation in a liver and blood serum interconnected with changes in the activity of the key antioxidant enzymes. The derivatives of succinic acid proved to inhibit the nitrogen oxide synthase reflected in a decrease in nitrite- and nitrate-anion level in a liver, blood plasma and urine as well as in changes in the oxidation processes in a liver and blood serum. We concluded in stating that the specified changes of pro-/antioxidant and nitrogen oxide homeostasis may serve as assessment criteria of the succinate-containing compounds’ biological action.