Ukr.Biochem.J. 2021; Volume 93, Issue 3, May-Jun, pp. 30-38

doi: https://doi.org/10.15407/ubj93.03.030

Administration of platelet-rich plasma or concentrated bone marrow aspirate after mechanically induced ischemia improves biochemical parameters in skeletal muscle

30.06.2021   Uncategorized   No comments

А. Т. Pidlisetsky1, G. V. Kosiakova2, T. M. Goridko2, A. G. Berdyschev2,
O. F. Meged2, S. I. Savosko3*, О. V. Dolgopolov4

1Lviv Regional Hospital for War Veterans and Repressed named after Yu. Lypa, Ukraine;
2Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
3Bogomolets National Medical University, Kyiv, Ukraine;
4SI “Institute of Traumatology and Orthopedics of NAMS of Ukraine”, Kyiv;
*e-mail: savosko_s@ukr.net

Received: 16 September 2020; Accepted: 17 May 2021

Ischemia is known to cause structural and functional damage to skeletal muscle. In this research platelet-rich plasma (PRP) and cell  bone marrow aspirate (CBMA) which are considered to be promising in  regenerative medicine were used to improve the animal muscles condition after ischemia. Mechanically induced ischemia (MII) of the rabbit skeletal muscles was caused by applying a tourniquet to the hind limb for 6 hours.  At 6 h after tourniquet was removed the rabbit PRP or CBMA solutions were injected into the tibia muscle and animals were divided into experimental groups (n = 15): 1 – ischemia + saline, 2 – ischemia + PRP, 3 – ischemia + CBMA. Intact animals were the control group (n = 5). Animals were removed from the experiment on 5, 15, and 30th days of reperfusion  after ischemia. A progressive hypotrophy, muscle fibers destruction and leukocyte infiltration was observed after tibia muscle MII. The ischemic state was accompanied by catalase and superoxide dismutase activation, persistent NO2– overproduction and a significant decrease in glutathione peroxidase activity in the muscle homogenates. It was shown that after PRP or CBMA local injection into the ischemic muscle catalase activity remained at the elevated level, SOD activity approached the control level and the most pronounced effects were NO2– level decrease and glutathioperoxidase activity restoration. After CBMA injection angiogenesis activation and the increase in the number of nuclei in the muscle fibrils were observed. The obtained data allow to expand the range of biochemical parameters for assessing the muscles condition after ischemia and indicate a positive effect of platelet-rich plasma and cell bone marrow aspirate injection on skeletal muscle regeneration.

Keywords: , , , , ,

References:

  1. Siemionow M, Arslan E. Ischemia/reperfusion injury: a review in relation to free tissue transfers. Microsurgery. 2004;24(6):468-475. PubMed, CrossRef
  2. Drysch M, Wallner C, Schmidt SV, Reinkemeier F, Wagner JM, Lehnhardt M, Behr B. An optimized low-pressure tourniquet murine hind limb ischemia reperfusion model: Inducing acute ischemia reperfusion injury in C57BL/6 wild type mice. PLoS One. 2019;14(1):e0210961. PubMed, PubMedCentral,  CrossRef
  3. Duehrkop C, Rieben R. Refinement of tourniquet-induced peripheral ischemia/reperfusion injury in rats: comparison of 2 h vs 24 h reperfusion. Lab Anim. 2014;48(2):143-154. PubMed, CrossRef
  4. Cunha MS, da Silva JC, Nakamoto HA, Ferreira MC. Study of warm ischemia followed by reperfusion on a lower limb model in rats: effect of allopurinol and streptokinase. Clinics (Sao Paulo). 2005;60(3):213-220. PubMed, CrossRef
  5. Yassin MM, Harkin DW, Barros D’Sa AA, Halliday MI, Rowlands BJ. Lower limb ischemia-reperfusion injury triggers a systemic inflammatory response and multiple organ dysfunction. World J Surg. 2002;26(1):115-121. PubMed, CrossRef
  6. Ekinci Akdemir FN, Gülçin İ, Karagöz B, Soslu R, Alwasel SH. A comparative study on the antioxidant effects of hesperidin and ellagic acid against skeletal muscle ischemia/reperfusion injury. J Enzyme Inhib Med Chem. 2016;31(sup4):114-118. PubMed, CrossRef
  7. Kılıç Y, Özer A, Tatar T, Zor MH, Kirişçi M, Kartal H, Dursun AD, Billur D, Arslan M, Küçük A. Effect of picroside II on hind limb ischemia reperfusion injury in rats. Drug Des Devel Ther. 2017;11:1917-1925. PubMed, PubMedCentral, CrossRef
  8. Bianco-Batlles MD, Sosunov A, Polin RA, Ten VS. Systemic inflammation following hind-limb ischemia-reperfusion affects brain in neonatal mice. Dev Neurosci. 2008;30(6):367-373. PubMed, PubMedCentral, CrossRef
  9. Ismail AM, Abdou SM, Aty HA, Kamhawy AH, Elhinedy M, Elwageh M, Taha A, Ezzat A, Salem HA, Youssif S, Salem ML. Autologous transplantation of CD34(+) bone marrow derived mononuclear cells in management of non-reconstructable critical lower limb ischemia. Cytotechnology. 2016;68(4):771-781. PubMed, PubMedCentral, CrossRef
  10. Liew A, O’Brien T. Therapeutic potential for mesenchymal stem cell transplantation in critical limb ischemia. Stem Cell Res Ther. 2012;3(4):28. PubMed, PubMedCentral, CrossRef
  11. Elshaer SL, Lorys RE, El-Remessy AB. Cell Therapy and Critical Limb Ischemia: Evidence and Window of Opportunity in Obesity. Obes Control Ther. 2016;3(1):121. PubMed, PubMedCentral, CrossRef
  12. Leroux L, Descamps B, Tojais NF, Séguy B, Oses P, Moreau C, Daret D, Ivanovic Z, Boiron JM, Lamazière JMD, Dufourcq P, Couffinhal T, Duplàa C. Hypoxia preconditioned mesenchymal stem cells improve vascular and skeletal muscle fiber regeneration after ischemia through a Wnt4-dependent pathway. Mol Ther. 2010;18(8):1545-1552. PubMed, PubMedCentral, CrossRef
  13. Rosová I, Link D, Nolta JA. shRNA-mediated decreases in c-Met levels affect the differentiation potential of human mesenchymal stem cells and reduce their capacity for tissue repair. Tissue Eng Part A. 2010;16(8):2627-2639. PubMed, PubMedCentral, CrossRef
  14. Setayesh K, Villarreal A, Gottschalk A, Tokish JM, Choate WS. Treatment of Muscle Injuries with Platelet-Rich Plasma: a Review of the Literature. Curr Rev Musculoskelet Med. 2018;11(4):635-642. PubMed, PubMedCentral, CrossRef
  15. Gaiovych I, Savosko S, Labunets I, Utko N, Makarenko A, Chaikovsky Y. Sciatic nerve regeneration after autografting and application of the bone marrow aspirate concentration. Georgian Med News. 2019;(295):145-152. PubMed
  16. Kamyshnikov VS. Reference Book on Clinic and Biochemical Researches and Laboratory Diagnostics. Moscow: MEDpress-inform, 2004 (In Russian).
  17. Gulaya NM, Kuzmenko AI, Margitich VM, Govseeva NM, Melnichuk SD, Goridko TM, Zhukov AD. Long-chain N-acylethanolamines inhibit lipid peroxidation in rat liver mitochondria under acute hypoxic hypoxia. Chem Phys Lipids. 1998;97(1):49-54. PubMed, CrossRef
  18. Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR. Analysis of nitrate, nitrite, and [15N]nitrate in biological fluids. Anal Biochem. 1982;126(1):131-138. PubMed, CrossRef
  19. Volchegorsky IA, Nalimov AG, Yarovinsky BG, Lifshitz RI. Different means of lipid peroxidation products estimation in heptane-isopropanol extracts of blood. Vopr Med Chim. 1989; 25(1):127-131.
  20. Nishikimi M, Appaji N, Yagi K. The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen. Biochem Biophys Res Commun. 1972;46(2):849-854. PubMed, CrossRef
  21. Hadwan MH, Abed HN. Data supporting the spectrophotometric method for the estimation of catalase activity. Data Brief. 2015;6:194-199. PubMed, PubMedCentral, CrossRef
  22. Pereslegina IA. The activity of antioxidant enzymes in the saliva of normal children. Lab Delo. 1989;(11):20-23. (In Russian). PubMed
  23. Oliver CN, Ahn BW, Moerman EJ, Goldstein S, Stadtman ER. Age-related changes in oxidized proteins. J Biol Chem. 1987;262(12):5488-5491. PubMed, CrossRef
  24. Weber D, Davies MJ, Grune T. Determination of protein carbonyls in plasma, cell extracts, tissue homogenates, isolated proteins: Focus on sample preparation and derivatization conditions. Redox Biol. 2015;5:367-380. PubMed, PubMedCentral,  CrossRef
  25. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72:248-254. PubMed, CrossRef
  26. Soares ROS, Losada DM, Jordani MC, Évora P, Castro-E-Silva O. Ischemia/Reperfusion Injury Revisited: An Overview of the Latest Pharmacological Strategies. Int J Mol Sci. 2019;20(20):5034. PubMed, PubMedCentral,  CrossRef
  27. Paradis S, Charles AL, Meyer A, Lejay A, Scholey JW, Chakfé N, Zoll J, Geny B. Chronology of mitochondrial and cellular events during skeletal muscle ischemia-reperfusion. Am J Physiol Cell Physiol. 2016;310(11):C968-C982. PubMed, PubMedCentral,  CrossRef
  28. Nozdrenko DM, Soroka VM, Khoma OM. Kinetics of single contractions inhibition under ischemia-reperfusion as an indicator of m. gastrocnemius functional state in rats. Fiziol Zh. 2019; 65(1): 50-56. CrossRef
  29. Dolgopolov OV, Nozdrenko DM, Strafun SS, Miroshnichenko MS. A change of skeletal muscles contraction parameters during the sharp ischemia. Physics Alive. 2010; 18(3): 64-69.
  30. Tran TP, Tu H, Pipinos II, Muelleman RL, Albadawi H, Li YL. Tourniquet-induced acute ischemia-reperfusion injury in mouse skeletal muscles: Involvement of superoxide. Eur J Pharmacol. 2011;650(1):328-334. PubMed, PubMedCentral, CrossRef
  31. Dong X, Xing Q, Li Y, Han X, Sun L. Dexmedetomidine protects against ischemia-reperfusion injury in rat skeletal muscle. J Surg Res. 2014;186(1):240-245. PubMed, CrossRef
  32. Televiak AT, Veresiuk TO, Selskyy PR, Volska AS, Kurylo KI, Nebesna ZM, Datsko TV, Boymystruk II. Dynamic ofindices of lipid peroxidation and antioxidant protection in muscular tissue and blood serum of rats with acute ischemia-reperfusion. J Educ Health Sport. 2018; 8(10): 245-255.
  33. DeMartino AW, Kim-Shapiro DB, Patel RP, Gladwin MT. Nitrite and nitrate chemical biology and signalling. Br J Pharmacol. 2019;176(2):228-245. PubMed, PubMedCentral, CrossRef
  34. Tsai WC, Yu TY, Chang GJ, Lin LP, Lin MS, Pang JHS. Platelet-Rich Plasma Releasate Promotes Regeneration and Decreases Inflammation and Apoptosis of Injured Skeletal Muscle. Am J Sports Med. 2018;46(8):1980-1986. PubMed, CrossRef
  35. Xie X, Wang Y, Zhao C, Guo S, Liu S, Jia W, Tuan RS, Zhang C. Comparative evaluation of MSCs from bone marrow and adipose tissue seeded in PRP-derived scaffold for cartilage regeneration. Biomaterials. 2012;33(29):7008-7018. PubMed, CrossRef
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