Tag Archives: skeletal muscle

Biochemical and tensometric analysis of C(60) fullerenes protective effect on the development of skeletal muscle fatigue

D. M. Nozdrenko1, K. I. Bogutska1, I. V. Pampuha1,
O. O. Gonchar2, O. M. Abramchuk3, Yu. I. Prylutskyy1*

1Taras Shevchenko National University of Kyiv, Ukraine;
*e-mail: prylut@ukr.net;
2Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv;
3Lesya Ukrainka Volyn National University, Lutsk, Ukraine

Received: 19 May 2021; Accepted: 07 July 2021

TThe protective effect of water-soluble C60 fullerenes on the development of slow and rapid fatigue of rat skeletal muscles was analyzed. It was found that the reduction of muscle contraction force (muscle soleus) by 50% of the initial values is almost twice as slow as stimulation with a frequency of 1 Hz (slow muscle fatigue) than with 2 Hz (rapid muscle fatigue) stimulation after intramuscular injection of C60  fullerenes (dose 0.5 mg/kg). There is a clear tendency to decrease the values of biochemical parameters of the blood of animals with the therapeutic effect of water-soluble C60 fullerenes by approximately 45-60% and 35-40% with the development of slow and rapid muscle fatigue, respectively. Thus, C60 fullerenes, as powerful antioxidants, are able to efficiently affect the prooxidant-antioxidant homeostasis of muscle tissue and thus help maintain its normal physiological state.

Administration of platelet-rich plasma or concentrated bone marrow aspirate after mechanically induced ischemia improves biochemical parameters in skeletal muscle

А. Т. Pidlisetsky1, G. V. Kosiakova2, T. M. Goridko2, A. G. Berdyschev2,
O. F. Meged2, S. I. Savosko3*, О. V. Dolgopolov4

1Lviv Regional Hospital for War Veterans and Repressed named after Yu. Lypa, Ukraine;
2Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
3Bogomolets National Medical University, Kyiv, Ukraine;
4SI “Institute of Traumatology and Orthopedics of NAMS of Ukraine”, Kyiv;
*e-mail: savosko_s@ukr.net

Received: 16 September 2020; Accepted: 17 May 2021

Ischemia is known to cause structural and functional damage to skeletal muscle. In this research platelet-rich plasma (PRP) and cell  bone marrow aspirate (CBMA) which are considered to be promising in  regenerative medicine were used to improve the animal muscles condition after ischemia. Mechanically induced ischemia (MII) of the rabbit skeletal muscles was caused by applying a tourniquet to the hind limb for 6 hours.  At 6 h after tourniquet was removed the rabbit PRP or CBMA solutions were injected into the tibia muscle and animals were divided into experimental groups (n = 15): 1 – ischemia + saline, 2 – ischemia + PRP, 3 – ischemia + CBMA. Intact animals were the control group (n = 5). Animals were removed from the experiment on 5, 15, and 30th days of reperfusion  after ischemia. A progressive hypotrophy, muscle fibers destruction and leukocyte infiltration was observed after tibia muscle MII. The ischemic state was accompanied by catalase and superoxide dismutase activation, persistent NO2 overproduction and a significant decrease in glutathione peroxidase activity in the muscle homogenates. It was shown that after PRP or CBMA local injection into the ischemic muscle catalase activity remained at the elevated level, SOD activity approached the control level and the most pronounced effects were NO2 level decrease and glutathioperoxidase activity restoration. After CBMA injection angiogenesis activation and the increase in the number of nuclei in the muscle fibrils were observed. The obtained data allow to expand the range of biochemical parameters for assessing the muscles condition after ischemia and indicate a positive effect of platelet-rich plasma and cell bone marrow aspirate injection on skeletal muscle regeneration.

Influence of C(60) fullerene on the ischemia-reperfusion injury in the skeletal muscle of rat limb: mechanokinetic and biochemical analysis

D. O. Zavodovskyi1, S. Yu. Zay2, T. Yu. Matvienko1, Yu. I. Prylutskyy1,
N. Y. Nurishchenko1, S. S. Paradizova3, L. L. Bezuh3, U. Ritter4, P. Scharff4

1ESC Institute of Biology and Medicine, Taras Shevchenko National University of Kyiv, Ukraine;
2Lesya Ukrainka Eastern European National University, Lutsk, Ukraine;
3SI The Territorial Medical Association of the Ministry of Internal Affairs of Ukraine in Kyiv;
4Institute of Chemistry and Biotechnology, Technical University of Ilmenau, Ilmenau, Germany;
e-mail: Lab@univ.kiev.ua

Influence of the pristine C60 fullerene aqueous colloidal solution (C60FAS) on the ischemia-reperfusion injury in the skeletal muscle of rat limb was studied. Skeletal muscle damage effects were induced by 3 h lasting­ vascular ischemia. The impact of C60FAS was studied after its intramuscular injection immediately after 1 h of reper­fusion at different doses, namely: 1, 2 and 3 mg/kg of body weight. Changes in the mechanokinetic parame­ters of ischemic skeletal muscle contraction at different modes of functioning and biochemical parame­ters of blood were used as markers of ischemic injury, and analyzed in detail under action of C60FAS. The obtained results indicate on great promise of use of C60FAS to reduce the consequences of ischemic muscle trauma.

ATPase activity of rabbit skeletal muscles actomyosin complex under ultrasound effect

K. O. Medynska, N. Ye. Nurishchenko, L. I. Pelyukh, O. V. Shelyuk

Taras Shevchenko Kyiv National University;
Educational and Scientific Centre “Institute of Biology”, Ukraine;
e-mail: medinkat@ukr.net

Influence of continuous and impulsive ultrasound 0.05; 0.2; 0.4; 0.7 and 1.0 W/cm2 on ATPase activity of rabbit skeletal muscle actomyosin has been investigated in this work. It has been shown that most changes of Mg2+,Ca2+-ATPase activity are observed under 0.2 and 0.4 W/cm2 continuous ultrasound. K+-ATPase activity is inhibited by continuous ultrasound of all intensities studied. Impulsive 2 and 10 ms ultrasound did not change the Mg2+,Ca2+-ATPase activity. While K+-activity is reliably changed only under impulsive 0.7 and 1.0 W/cm2 ultrasound that can be explained by the thermal effect. It has been determined, when studying­ the reconstructed actomyosin with sound troponin complex, that troponin complex is the most ultrasound sensitive constituent of actomyosin.

Stoichiometry of cytochromes and oxygen tension in skeletal muscles of marine fish

A. A. Soldatov1, I. A. Parfyonova2

1A. O. Kovalevsky Institute of Biology of the Southern Seas,
National Academy of Science of Ukraine, Sevastopol;
e-mail: alekssoldatov@yandex.ru;
2V. I. Vernadsky Taurida National University, Simferopol

The character of oxygen tension distribution and peculiarities of cytochromes stoichiometry in skeletal muscles of bottom and pelagic species of marine fish were compared. It is shown, that the limitation of muscle activity increases the number of hypoxic zones in the muscle tissue. The mitochondrial electron-transporting chain then obtain the uncompensated type of organization, expressed in the increase of the share of the terminal complex aa3 on the background of general reduction of cytochromes content in the muscles. The reaction is of an adaptive character and can be implemented by pelagic fish species in conditions of experimental hypokinesia.