Ukr.Biochem.J. 2014; Volume 86, Issue 2, Mar-Apr, pp. 60-67

doi: http://dx.doi.org/10.15407/ubj86.02.060

Stoichiometry of cytochromes and oxygen tension in skeletal muscles of marine fish

A. A. Soldatov1, I. A. Parfyonova2

1A. O. Kovalevsky Institute of Biology of the Southern Seas,
National Academy of Science of Ukraine, Sevastopol;
e-mail: alekssoldatov@yandex.ru;
2V. I. Vernadsky Taurida National University, Simferopol

The character of oxygen tension distribution and peculiarities of cytochromes stoichiometry in skeletal muscles of bottom and pelagic species of marine fish were compared. It is shown, that the limitation of muscle activity increases the number of hypoxic zones in the muscle tissue. The mitochondrial electron-transporting chain then obtain the uncompensated type of organization, expressed in the increase of the share of the terminal complex aa3 on the background of general reduction of cytochromes content in the muscles. The reaction is of an adaptive character and can be implemented by pelagic fish species in conditions of experimental hypokinesia.

Keywords: , , ,


References:

  1. Middelburg JJ, Levin L A. Coastal hypoxia and sediment biogeochemistry. Biogeosciences. 2009;6(7):1273–1293. CrossRef
  2. Gewin V. Oceanography: Dead in the water. Nature. 2010 Aug 12;466(7308):812-4. PubMed, CrossRef
  3. Van Waarde A. Biochemistry of non-protein nitrogenous compounds in fish including the use of amino acids for anaerobic energy production. Comp Biochem Physiol Part B. 1988;.91(2):207-228. CrossRef
  4. Shulman GE, Abolmasova GI, Stolbov AYa. The use of protein in the energy metabolism of aquatic organisms. Uspekhi Sovrem Biologii. 1993;113:576-586.
  5. Chew SF, Gan J, Ip YK. Nitrogen metabolism and excretion in the swamp eel, Monopterus albus, during 6 or 40 days of estivation in mud. Physiol Biochem Zool. 2005 Jul-Aug;78(4):620-9. PubMed, CrossRef
  6. Owen TG, Hochachka PW. Purification and properties of dolphin muscle aspartate and alanine transaminases and thier possible roles in the energy metabolism of diving mammals. Biochem J. 1974 Dec;143(3):541-53. PubMed, PubMedCentralCrossRef
  7. Mommsen TP, French CJ, Hochachka PW. Sites and patterns of protein and amino acid utilization during the spawning migration of salmon. Can J Zool. 1980;58(10):1785-1799. CrossRef
  8. Soldatov AA. Organ blood flow and microcirculatory vessels in fish. Zh Evol Biokhim Fiziol. 2006 May-Jun;42(3):193-200. Russian. PubMed
  9. Shulman GE, Love RM. The Biochemical Ecology and Marine Fishes / Adv. Mar. Biol. – London: Academic press, 1999. 36. 347 p.
  10. Soldatov AA. The experimental study of the distribution of oxygen tension in the muscle tissue of marine fish. Zh Evol Biokhim Fiziol. 1993; 26(5,6):656-659.
  11. Houston AH. Blood and circulation. Methods for fish biology. N-Y.: Amer. Fish. Society, 1990. P. 273-334.
  12. Soldatov AA. Physiological effects of urethane anesthesia on the organism of marine fish. Gidrobiol Zhurn. 2003; 39(1):51-63.
  13. Berezovskiy VA. The oxygen tension in the tissues of animals and humans. Kiev: Nauk. dumka, 1975. 276 p.
  14. Evdotienko YuV, Mohova EN. Mechanisms of respiration, photosynthesis and nitrogen fixation. M.: Nauka, 1967. 154 p.
  15. Van Handel PJ, Sandel WR, Mole PA. Effects of exogenous cytochrome c on respiratory capacity of heart and skeletal muscle. Biochem Biophys Res Commun. 1977 Feb 7;74(3):1213-9. PubMed
  16. Rebrov OYu. Statistical analysis of medical data. The use of the software STATISTIKA. M.: Media Sfera, 2002. 305 p.
  17. Belaud A, Trotter Y, Peyraud C. Continuous evaluation of Pa,O2 in fish: recording and data processing. J Exp Biol. 1979 Oct;82:321-30. PubMed
  18. McKenzie DJ, Wong S, Randall DJ, Egginton S, Taylor EW, Farrell AP. The effects of sustained exercise and hypoxia upon oxygen tensions in the red muscle of rainbow trout. J Exp Biol. 2004 Oct;207(Pt 21):3629-37. PubMed, CrossRef
  19. Belokopyitin YuS. Energy metabolism in marine fish. Kiev: Nauk. dumka, 1993. 127 p.
  20. Kakuno A, Sezaki K, Ikeda Y.  Bull Natl Res Inst Fish Sci Japan.  1996;8:15-27.
  21. Savina MV. Mechanisms of adaptation of tissue respiration in the evolution of vertebrates. St.‑Peterburg: Nauka, 1992. 200 p.
  22. Soldatov AA, Savina MV. Effect of hypoxia on the content and stoichiometry of cytochromes in muscle of the gray mullet Liza aurata. Zh Evol Biokhim Fiziol. 2008 Sep-Oct;44(5):508-12. Russian. PubMed

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License.