Tag Archives: protein C
C(60) fullerene effect on the functional activity of rat gastrocnemius muscle during its regeneration after the open injury
D. M. Nozdrenko1, O. O. Gonchar2, N. E. Nurishchenko1,
V. O. Stetska1, T. Yu. Matviienko1, Ya. V. Stepanyuk3,
K. I. Bogutska1, Yu. I. Prylutskyy1*
1ESC “Institute of Biology and Medicine”, Taras Shevchenko National University of Kyiv, Ukraine;
2Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv;
3Medical Faculty, Lesya Ukrainka Volyn National University, Lutsk, Ukraine;
*e-mail: prylut@ukr.net
Received: 05 September 2024; Revised: 14 October 2024;
Accepted: 21 November 2024; Available on-line: 17 December 2024
Open injuries are one of the most common skeletal muscle traumas. The study aimed to estimate the effect of the oral administration of C60 fullerene aqueous solution (C60FAS) daily at a dose of 1 mg/kg on the restoration of rat skeletal muscle functional activity on the 5th, 10th and 15th day after the open trauma. Male Wistar rats were randomly divided into three groups of 12 animals in each: control, with muscle injury and with muscle injury+C60FAS. The isolated gastrocnemius muscle was subjected to open injury by transverse dissection with a depth of 1 mm. Stimulation of muscle efferents was carried out by electrical impulses generated using a strain gauge generator. The content of C-reactive protein, creatinine, lactate, reduced glutathione and the activity of catalase and superoxide dismutase in the rat blood were determined. According to the data obtained, application of C60FAS promotes the restoration of the functional activity of injured muscle, which was confirmed by a significant increase in gastrocnemius muscle force impulse, attenuation of the inflammatory and development of fatigue and normalization of pro- and antioxidant balance in the process of regeneration.
Characterization of the blood coagulation system in morbidly obese patients
D. S. Korolova1, O. V. Hornytska1, A. S. Lavrik2,
N. M. Druzhyna1*, N. Prysyazhna3, T. M. Platonova1
1Palladin Institute of Biochemistry, National Academy of Science of Ukraine, Kyiv;
2Shalimov National Institute of Surgery and Transplantation,
National Academy of Medical Sciences of Ukraine, Kyiv;
3Shupyk National Healthcare University of Ukraine, Kyiv;
*e-mail: ndbiochem@gmail.com
Received: 18 April 2023; Revised: 05 May 2023;
Accepted: 7 September 2023; Available on-line: 12 September 2023
Obesity is a complex metabolic disorder that can be followed by blood coagulation disorders, atherosclerosis and atherothrombosis. In the present work, the levels of fibrinogen, soluble fibrin, D-dimer as well as protein C were measured in the blood plasma of 24 morbidly obese patients (the body mass index exceeds 40 kg/m2) to evaluate the risk of prothrombotic state. The study showed that near by 80% of patients had substantially increased fibrinogen concentration, 33% had increased concentration of soluble fibrin, 42% had increased level of D-dimer in blood plasma as compared to control. According to the results of individual analysis, the high level of fibrinogen and soluble fibrin while reduced protein C indicated the threat of thrombosis, which requires complex diagnostics to be identified. Therefore, simultaneous quantification of hemostatic system biomarkers in the blood plasma is the confident way to predict the risk of thrombotic complications in morbidly obese patients.
Overall hemostasis potential of the blood plasma and its relation to some molecular markers of the hemostasis system in patients with chronic renal disease of stage VD
B. G. Storozhuk1, L. V. Pyrogova2, T. M. Chernyshenko2, O. P. Kostiuchenko2,
I. M. Kolesnikova2, T. M. Platonova2, O. B. Storozhuk1, L. O. Storozhuk1,
G. K. Bereznitsky2, P. Yu. Tsap2, O. O. Masenko2,
E. M. Makogonenko2, E. V. Lugovskoy2
1Pyrogov National Medical University of Vinnytsa, Ukraine;
2Palladin Instiute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
e-mail: makogonenko@biochem.kiev.ua
The values of the coagulation, overall and fibrinolysis potentials were estimated by the method of the global potential of Blomback M., as well as the values of concentrations of molecular markers of the hemostasis system: soluble fibrin (sf), D-dimer, fibrinogen (Fg) and protein C (88 patients, 52 of them men, 36 women). It was shown that hemostasis system activity in women plasma is higher than that in men plasma. The division of patients into 3 groups, depending on the concentration of sf: less than normal – sf ≤ 3, about norm – 3 < sf < 4 and more than norm – sf > 4 μg/ml, allowed establishing the growth of the parameters of both the hemostatic potential and concentrations of molecular markers in accordance with concentration of sf in the groups of patients. Paerson’s correlation analysis of the relationship between the parameters of the hemostasis potential and concentrations of molecular markers revealed an increase in the correlation relationship to the strong and very strong between the parameters of coagulation, fibrinolysis and protein C systems with an increase in the concentration of soluble fibrin in plasma of patients.
Recombinant single chain variable fragment antibodies (scFv) against Pro(144)-Leu(155) fragment of human protein C
O. S. Oliinyk, K. O. Palyvoda, N. E. Lugovskaya,
D. V. Kolibo, E. V. Lugovskoy, S. V. Komisarenko
Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
e-mail: lenaoliinyk@mail.ru
The aim of this work was to obtain the recombinant single chain variable fragments of antibodies (scFv) against human protein C, the key component of blood anticoagulation system. For this purpose a peptide that mimics a Pro144-Leu155 sequence of protein C was synthesized and the murine immune scFv library against this peptide was constructed. The protein C specific scFv 9E were selected from the constructed library by the phage-display method. The scFv 9E dissociation constant was found to be 2∙10-9 М. It was shown that scFv 9E were suitable for protein C detection by ELISA and Western blotting. Selected scFv could be further used for protein C investigation and for the development of quantitative methods for protein C detection in human blood.